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521 items found for "Jespers W"
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- Signaling pathways activated by sea bass gonadotropin-inhibitory hormone peptides in COS-7 cells...
functional gonadotropin-inhibitory hormone (GnIH) system in the European sea bass, Dicentrarchus labrax , which Herein, we further elucidated the intracellular signaling pathways mediating in sea bass GnIH actions Moreover, an evident increase in SRE-luc activity was noticed when COS-7 cells expressing GnIHR were
- Structure of Mycobacterium tuberculosis Cya, an evolutionary ancestor of the mammalian membrane...
Although the vital role of ACs in cellular signalling is well established, the function of their transmembrane Here, we describe the cryo-EM structure of Cya bound to a stabilizing nanobody at 3.6 Å resolution.
- CCL25/CCR9 interaction promotes the malignant behavior of salivary adenoid cystic carcinoma via...
This study aimed to investigate the specific molecular mechanism by which CCR9/CCL25 modulates malignant Methods Immunohistochemistry staining and RT–qPCR analyses were performed to detect the correlation In vitro , SACC cell proliferation and apoptosis were evaluated using Cell Counting Kit-8 and colon formation , and cell migration and invasion were detected by wound healing and transwell assays. Western blot and RT–qPCR assays were carried out to measure the downstream factors of the interaction
- Fentanyl activates ovarian cancer and alleviates chemotherapy-induced toxicity via opioid...
via opioid receptor-dependent activation of EGFR "Background Fentanyl is an opioid analgesic and is widely Methods Proliferation, migration and apoptosis assays were performed in ovarian cancer cells after
- Multifunctional role of GPCR signaling in epithelial tube formation
Here, we reveal that the Smog GPCR transduces Fog signal to regulate Rho kinase accumulation and myosin We also report on unexpected Fog-independent roles for Smog in maintaining epithelial integrity and organizing Our data support a model wherein Smog regulates distinct myosin pools and actin cytoskeleton in a ligand-dependent
- Neuropeptide S Encodes Stimulus Salience in the Paraventricular Thalamus
Here we report that neuropeptide S (NPS) signaling in the PVT is necessary for stimulus salience encoding receptor (NPSR1) and NPS precursor knockout mice in fear extinction or novel object memory formation, we phenotype in NPS precursor knockout mice by increasing the salience of otherwise low-intensity stimuli, while intra-PVT injections of NPSR1 antagonist in wild type mice partially replicates the knockout phenotype were previously linked to the brain salience network.
- C1-inhibitor influence on platelet activation by thrombin receptors agonists
Here we show that C1INH affects platelet activation through PAR1 and PAR4 agonists. Methods Platelets were isolated from healthy donor whole blood and then labeled with anti-CD62P and PAC1 The platelet suspensions were exposed to PAR1 agonists SFLLRN, TFLLR and TFLLRN; PAR4 agonists AYPGKF Flow-cytometric measurements were performed in 5, 10 and 15 min after activation."
- Delineation of GPR15 receptor-mediated Gα protein signaling profile in recombinant mammalian cell
September 2022 "The GPR15 receptor is a G protein-coupled receptor (GPCR), which is activated by an endogenous However, the activation profiles of the GPR15 receptor within Gi/o subtypes have not been examined. Moreover, whether the receptor can also couple to Gs , Gq/11 and G12/13 is unclear. Within the Gi/o family, the GPR15 receptor activates all the subtypes (Gi1 , Gi2 , Gi3 , GoA , GoB and The Emax and activation rates of Gi1, Gi2 , Gi3, GoA and GoB are similar, whilst the Emax of Gz is smaller
- Residency time of agonists does not affect the stability of GPCR-arrestin complexes
GPCRs that internalize without arrestin have been classified as "class A" GPCRs whereas "class B" GPCRs Here, we ask the question whether agonists with very slow off-rates can cause the formation of particularly
- Lack of Oestrogen Receptor Expression in Breast Cancer Cells Does Not Correlate with Kisspeptin...
To assess the veracity of this claim, we compared endogenous KISS1R signalling and physiological output MDA-MB-231 cells are metastatic when implanted in mice while BT-20 are not and remain epithelial-like We show that both cell lines express KISS1R mRNA and respond to KP-10 by elevating calcium mobilisation true that kisspeptin/KISS1R stimulate migration or pro-metastatic behaviour, as divergent responses were Whether this divergence is related to the observed differences in β-arrestin complements warrants further
- GRK3 is a poor prognosticator and serves as a therapeutic target in advanced gastric adenocarcinoma
Methods We studied GRK3 expression in normal, primary, and metastatic GAC tissues. We identified a novel GRK3 inhibitor, LD2, through a chemical-library screen. Through genetic and pharmacologic modulations of GRK3, a series of functional and molecular studies were
- C5aR2 receptor: The genomic twin of the flamboyant C5aR1
The C5aR1 is a classical G-protein-coupled receptor (GPCR), whereas C5aR2 is a nonclassical GPCR that The functional relevance of C5aR2 appears to be context-dependent compared to the C5aR1, which has received study are expected to further enrich the understanding of C5a-C5aR2 interaction compared to C5a-C5aR1, which
- Chemerin Forms: Their Generation and Activity
September 2022 "Chemerin is the product of the RARRES2 gene which is secreted as a precursor of 143 That precursor is inactive, but proteases from the coagulation and fibrinolytic cascades, as well as Chemerin can signal via two G protein-coupled receptors, chem1 and chem2, as well as be bound to a third the liver and secreted into the circulation as a precursor, but it is also expressed in some tissues where
- Microbial Metabolites Orchestrate a Distinct Multi-Tiered Regulatory Network in the Intestinal Epith
We now aim to determine the molecular mechanism by which this functional microbiome output regulates Using shRNA knockdown and CRISPR/Cas9 knockout cell lines, as well as mouse models, we confirmed the
- Endothelin-1 Stimulates PAI-1 Protein Expression via Dual Transactivation Pathway Dependent ROCK...
to the carboxy region, Smad2 transcription factor can be phosphorylated in the linker region as<br />well linker region (Smad2L) promotes the expression of plasminogen activator inhibitor type<br />1 (PAI-1) which addition, as an intermediary of G protein-coupled receptor (GPCR) signaling, the functions of ROCK and PLC were Proteins were investigated<br />by western blotting using protein-specific antibodies against phospho-Smad2
- Structure of the human galanin receptor 2 bound to galanin and Gq reveals the basis of ligand...
G-protein interface "Galanin is a neuropeptide expressed in the central and peripheral nervous systems, where Three G-protein coupled receptors (GPCRs) for galanin have been discovered, which is the focus of efforts understand the basis of the ligand preferences of the receptors and to assist structure-based drug design, we Mutant proteins were assayed to help reveal the basis of ligand specificity, and structural comparison
- Regulator of G Protein Signaling 20 Correlates with Long Intergenic Non-Coding RNA (lincRNAs)...
Interestingly, RGS (Regulators of G protein signaling) proteins, which negatively regulate GPCR signaling associated with various cancers, with some members of the RGS family being associated with liver cancer as well Considering this, we investigated the role of RGS20 as a potential prognostic marker in 28 different Taken together, we showed that RGS20 correlates with a few HCC-associated lincRNAs harboring oncogenic
- Neurotransmitters: Potential Targets in Glioblastoma
Here, we provide an overview of the most novel advances concerning the role of neurotransmitters in the
- Ginsenoside Rg5 allosterically interacts with P2RY12 and ameliorates deep venous thrombosis by...
DVT formation is a time-dependent inflammatory process in which NETosis plays an important role. However, whether ginsenoside Rg5 from species of Panax genus could alleviate DVT and its underlying mechanism
- Expression pattern and clinical significance of beta 2-adrenergic receptor in oral squamous cell...
Beta 2-Adrenergic Receptor (β2-AR) is significantly overexpressed in various types of malignancies, which is associated with the worst prognosis. However, the role of β2-AR in oral cancer is not well identified. Methods: Immunohistochemistry, western blot and quantitative real-time PCR techniques were used to analyze
- Cell-Type-Specific Effects of the Ovarian Cancer G-Protein Coupled Receptor (OGR1) on Inflammation..
The pathophysiology is not fully understood, but the working hypothesis postulates that a combination We previously demonstrated that a reduction in extracellular pH activates latent TGF-β1, and that TGF-β1 Given the important roles of extracellular pH in the progression of pulmonary fibrosis, we sought to identify whether pH mediates other cellular phenotypes independent of TGF-β1. Here, we report that the Ovarian Cancer G-Protein Coupled Receptor1 (OGR1 or GPR68) has dual roles in
- G protein coupling and activation of the metabotropic GABAB heterodimer
Here, we use molecular metadynamics computations to predict the mechanism by which the inactive GP induces We find that the inactive GP first interacts with TM3, which further leads to the TMD rearrangement and