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255 items found for "Keywords: B cell migration"

  • From outside to inside and back again: the lysophosphatidic acid-CCN axis in signal transduction

    a lipid that activates G protein-coupled receptors (GPCRs), enhancing proliferation, adhesion, and migration in many types of cancer cells. Our group previously reported that LPA induces production of CCN1 protein in human prostate cancer cell In these cells, the mitogenic activity of LPA is mediated by LPA Receptor 1 (LPAR1), a GPCR. In some model systems, CCN1 and CCN2 play key roles in LPA/S1P-induced cell migration and proliferation

  • Purinergic GPCR-integrin interactions drive pancreatic cancer cell invasion

    News < GPCRs in Oncology and Immunology Purinergic GPCR-integrin interactions drive pancreatic cancer cell P2RY2 presents as the purinergic gene with the strongest association with hypoxia, the highest cancer cell-specific receptor-integrin interactions were required for effective downstream signalling, leading to cancer cell Hemant M Kocher , Sabrina Simoncelli , Peter J McCormick , Richard Philip Grose Tags cancer biology , cell Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call for GPCR

  • NPFF stimulates human ovarian cancer cell invasion by upregulating MMP-9 via ERK1/2 signaling

    < GPCR News < GPCRs in Oncology and Immunology NPFF stimulates human ovarian cancer cell invasion by The TaqMan probe-based RT-qPCR showed that NPFF and NPFFR2 were expressed in three human EOC cells, CaOV3 In comparison, NPFF and NPFFR2 expression levels were higher in SKOV3 cells than in CaOV3 or OVCAR3 cells Treatment of SKOV3 cells with NPFF did not affect cell viability and proliferation but stimulated cell This study provides evidence that NPFF stimulates EOC cell invasion by upregulating MMP-9 expression

  • The pyruvate-GPR31 axis promotes transepithelial dendrite formation in human intestinal dendritic cells

    Immunology The pyruvate-GPR31 axis promotes transepithelial dendrite formation in human intestinal dendritic cells intestinal lumen is rich in gut microbial metabolites that serve as signaling molecules for gut immune cells by the gut bacterial metabolite pyruvate, is specifically expressed on type 1 conventional dendritic cells Using human induced pluripotent stem cell-derived cDC1s and a monolayer human gut organoid coculture Kumanogoh, Kiyoshi Takeda Tags G protein-coupled receptors , GPR31 , antigen recognition , dendritic cell

  • The GPCR-Gαs-PKA signaling axis promotes T cell dysfunction and cancer immunotherapy failure

    < GPCR News < GPCRs in Oncology and Immunology The GPCR-Gαs-PKA signaling axis promotes T cell dysfunction Here, we cross-integrated large singe-cell RNA-sequencing datasets from CD8+ T cells covering 19 distinct cancer types and identified an enrichment of Gαs-coupled GPCRs on exhausted CD8+ T cells. These include EP2, EP4, A2AR, β1AR and β2AR, all of which promote T cell dysfunction. Gαs-DREADD to activate CD8-restricted Gαs signaling and show that a Gαs-PKA signaling axis promotes CD8+ T cell

  • Molecular characterization of breast cancer cell pools with normal or reduced ability to respond to progesterone: a study based on RNA-seq

    < GPCR News < GPCRs in Oncology and Immunology Molecular characterization of breast cancer cell pools Methods: We disrupted the PR gene (PGR) in ERα-positive/PR-positive T-47D cells using the CRISPR/Cas9 to control T-47D cells. We analyzed the gene expression profiles of PR-low and control T-47D cells in the absence of hormone More than 6000 genes were DE in control T-47D cells upon stimulation with P4 or P4 plus E2.

  • Opposite Effects of Src Family Kinases on YAP and ERK Activation in Pancreatic Cancer Cells: Implications for Targeted Therapy

    and Immunology Opposite Effects of Src Family Kinases on YAP and ERK Activation in Pancreatic Cancer Cells IGF-1 receptors and G protein-coupled (GPCR) signaling systems leading to mitogenic signaling in PDAC cells agonist neurotensin induced rapid activation of Src family of tyrosine kinases (SFK) within PANC-1 cells by FAK phosphorylation at Tyr576/577 and Tyr861, sensitive biomarkers of SFK activity within intact cells and suppressed the growth of Mia PaCa-2 cells xenografted into the flank of nude mice.

  • G Protein-coupled Receptor-mediated Membrane Targeting of PLCγ2 is Essential for Neutrophil Chemotaxis

    constitutes GPCR-mediated phospholipase C (PLC) signaling and is essential for neutrophil polarization and migration In response to a chemoattractant stimulation, cells lacking PLCγ2 (plcg2kd) displayed altered dynamics phosphorylation and cofilin activation; impaired dynamics of actin polymerization; and consequently, defects in cell polarization and migration during chemotaxis. Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call for GPCR

  • RGS20 promotes non-small cell lung carcinoma proliferation via autophagy activation and inhibition of the PKA-Hippo signaling pathway

    < GPCR News < GPCRs in Oncology and Immunology RGS20 promotes non-small cell lung carcinoma proliferation CCK8 and cell cloning were conducted to determine the proliferation ability of H1299 and Anip973 cells Further, RGS20 accelerated cell proliferation by increasing autophagy. in RGS20 knock-down cells. Conclusion: Our findings indicate that RGS20 drives NSCLC cell proliferation by triggering autophagy

  • Single cell G-protein coupled receptor profiling of Transcription factor 21 expressing activated kidney fibroblasts

    < GPCR News < GPCRs in Oncology and Immunology Single cell G-protein coupled receptor profiling of Transcription Key results: Transcription factor 21 (Tcf21)+ cells that expressed 2 or 3 of Postn, Acta2 and Pdgfra Tcf21+ α-smooth muscle actin (α-SMA)+ interstitial cells accumulated in the kidneys of mice with UUO TCF21, ADGRA2, S1PR3 and ADORA2A/2B were each detectable in α-SMA+ interstitial cells in human kidneys Study of GPCRs expressed by these cells may identify new opportunities for CKD therapeutics. " Authors

  • Stimulation of ectopically expressed muscarinic receptors induces IFN-γ but suppresses IL-2 production by inhibiting activation of pAKT pathways in primary T cells

    induces IFN-γ but suppresses IL-2 production by inhibiting activation of pAKT pathways in primary T cells signaling molecules and the phosphatidylinositol, Ras, MAPK, and PI3 kinase pathways, leading to T cell This may explain the inhibitory impact on IL-2 production in hM3Dq/β1T cells. that activating the pAKT pathway is critical for IL-2 production in T cells." Tags GPCR; T cells; muscarinic receptor; signaling.

  • Drosophila cytokine GBP2 exerts immune responses and regulates GBP1 expression through GPCR receptor Mthl10

    , we demonstrate that GBP2 replicates the GBP1-mediated cellular immune response from Drosophila S2 cells Furthermore, treatment of S2 cells with GBP2 enhanced GBP1 expression levels, but GBP1 did not affect GBP2-induced enhancement of GBP1 expression was not observed in Mthl10 knockdown cells. Enhancement of GBP2 expression was observed in both Drosophila larvae and S2 cells under heat stress Finally, Ca2+ mobilization assay in GCaMP3-expressing S2 cells demonstrated that GBP2 mobilizes Ca2+

  • GPR15 expressed in T lymphocytes from RA patients is involved in leukocyte chemotaxis to the synovium

    2022 Abstract The rheumatoid arthritis (RA) inflammatory process occurs in the joints where immune cells as to perform a functional migration assay in response to GPR15L. synovial fluid and peripheral blood were analyzed for CD45+CD3+CD4+GPR15+ and CD45+CD3+CD8+GPR15+ T cell Migration assays were performed using PBMCs isolated from these individuals in response to the synthetic GPR15 and GPR15L are present in the synovial tissue of RA patients and GPR15L promotes migration of PBMCs

  • Distinct Activation Mechanisms of CXCR4 and ACKR3 Revealed by Single-Molecule Analysis of their Conformational Landscapes

    atypical receptor ACKR3 both respond to CXCL12 but induce different effector responses to regulate cell migration. While CXCR4 couples to G proteins and directly promotes cell migration, ACKR3 is G protein-independent regulate extracellular chemokine levels and maintain CXCR4 responsiveness, thereby indirectly influencing migration single-molecule FRET Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

  • Comparative analysis of the occupancy of Histone H3 Lysine 4 methylation in the cells treated with TGFβ and Interferonγ

    Oncology and Immunology Comparative analysis of the occupancy of Histone H3 Lysine 4 methylation in the cells 28, 2023 Abstract "In this current study, we have compared our H3K4me3 Chip-Sequencing data in PC3 cells in response to 6h and 24h TGFβ stimulation with the IFNγ stimulated/unstimulated HeLa S3 cells Since modification changes in response to TGFβ and IFNγ and compare them to explore the genes common to both as well Noopur Thakur Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

  • Metallo-protease Peptidase M84 from Bacillusaltitudinis induces ROS-dependent apoptosis in ovarian cancer cells by targeting PAR-1

    Metallo-protease Peptidase M84 from Bacillusaltitudinis induces ROS-dependent apoptosis in ovarian cancer cells This metallo-protease had no discernible impact on normal cell survival, but it specifically induced apoptosis in ovarian cancer cells. PAR-1, a GPCR which is reported to be overexpressed in ovarian cancer cells, was identified as a target This evoked apoptotic death of the ovarian cancer cells through the intrinsic route.

  • Comparison of infectious complications with BCMA-directed therapies in multiple myeloma

    complications with BCMA-directed therapies in multiple myeloma Published date May 31, 2024 Abstract "B-cell-maturation-antigen single-center analysis we evaluated infectious complications after BCMA-targeted chimeric-antigen-receptor T-cell Comparing T-cell redirecting therapies, the incidence rate of severe infections was significantly lower Almeida Costa, Noriko Nishimura, Tasmin Farzana, Colin Rueda, David J Chung, Heather J Landau, Oscar B M Lesokhin Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

  • Enterococcus-derived tyramine hijacks α2A-adrenergic receptor in intestinal stem cells to exacerbate colitis

    Oncology and Immunology Enterococcus-derived tyramine hijacks α2A-adrenergic receptor in intestinal stem cells disease (IBD) is characterized by dysbiosis of the gut microbiota and dysfunction of intestinal stem cells Using an engineered tyrDC-deficient Enterococcus faecalis strain and intestinal epithelial cell-specific adrenergic receptor Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

  • miR-19a may function as a biomarker of oral squamous cell carcinoma (OSCC) by regulating the signaling pathway of miR-19a/GRK6/GPCRs/PKC in a Chinese population

    < GPCR News < GPCRs in Oncology and Immunology miR-19a may function as a biomarker of oral squamous cell Using a transcriptomic analysis of over 37,000 nuclei, we identified twelve distinct clusters of cells corresponding to temperature-sensing arista neurons, humidity-sensing sacculus neurons, and support cells We found that each cell type could be characterized by a unique expression profile of ion channels, GPCR signaling molecules, synaptic vesicle cycle proteins, and cell adhesion molecules.

  • A GPCR checkpoint drives CD8+ T cell dysfunction and immunotherapy failure in mice

    < GPCR News < GPCRs in Oncology and Immunology A GPCR checkpoint drives CD8+ T cell dysfunction and immunotherapy Authors listed Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

  • [1,2,4]Triazolo[1,5-c]pyrimidines as Tools to Investigate A3 Adenosine Receptors in Cancer Cell Lines

    Immunology [1,2,4]Triazolo[1,5-c]pyrimidines as Tools to Investigate A3 Adenosine Receptors in Cancer Cell Compound 20 has been tested on both A3 adenosine receptor positive cancer cell lines (CHO-A3AR transfected , THP1 and HCCT26) and in A3 negative cancer cell lines, showing no effect on the latter and a proliferative antagonists Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

  • CaaX-motif-adjacent residues influence G protein gamma (Gγ) prenylation under suboptimal conditions

    modification that supports membrane interactions of proteins involved in various cellular processes, including migration We examined the prenylation of carboxy-terminus (Ct) mutated Gγ in cells exposed to Fluvastatin and prenyl monitored the subcellular localization of fluorescently tagged Gγ subunits and their mutants using live-cell Given the cell and tissue-specific expression of different Gγ subtypes, our findings indicate a plausible mechanism allowing for statins to differentially perturb heterotrimeric G protein signaling in cells

  • In Silico Design of Novel RGS2-Galpha-q Interaction Inhibitors with Anticancer Activity

    Docking of identified hits to RGS2 as well as other RGS structures was used to screen the hits for potent Whole cell assays showed the top 10 ranking compounds, AJ-1-AJ-10, to inhibit RGS2-Gαq interactions. All 10 compounds inhibited the growth of several RGS2 expressing cancers in cell culture assays. In addition, AJ-3 inhibited the migration of LNCaP prostate cancer cells in wound healing assays. M Mahfouz Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

  • Regulator of G protein signaling protein 6 alleviates acute lung injury by inhibiting inflammation and promoting cell self-renewal in mice

    G protein signaling protein 6 alleviates acute lung injury by inhibiting inflammation and promoting cell Organoid culture was used to assess the stemness and self-renewal capacity of alveolar epithelial type II cells in macrophages and decreased apoptosis in epithelial cells. a protective role in ALI not only in early inflammatory responses but also in endogenous lung stem cell Zhou , Linlin Wang , Yu Yan , Jun She , Lin Tong , Yuanlin Song Tags Acute lung injury , Apoptosis , Cell-renewal

  • PAXIP1-AS1 is associated with immune infiltration and predicts poor prognosis in ovarian cancer

    long non-coding RNA (LncRNA) PAXIP1 antisense RNA 1 (PAXIP1-AS1) was found to promote proliferation, migration , EMT, and apoptosis of ovarian cancer (OC) cells in OC cell lines, but the relationship between PAXIP1 QRT-PCR was used to validate the expression of PAXIP1-AS1 in OC cell lines. PAXIP1-AS1 was significantly downregulated in OC cell lines compared with IOSE29 cell line. , Hui Liu Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

  • Prediction of survival and immunotherapy response by the combined classifier of G protein-coupled receptors and tumor microenvironment in melanoma

    Flowcytometry and Transwell assays were performed to observe the changes in cell apoptosis, metastasis and PKC via the signaling pathway of miR-19a/GRK6/GPR39/PKC, which accordingly resulted in suppressed cell apoptosis and promoted cell migration and invasion. scRNA-seq Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

  • GprC of the nematode-trapping fungus Arthrobotrys flagrans activates mitochondria and reprograms fungal cells for nematode hunting

    of the nematode-trapping fungus Arthrobotrys flagrans activates mitochondria and reprograms fungal cells GPCR, GprC, at the plasma membrane and together with the G-protein alpha subunit GasA, reprograms the cell Reinhard Fischer Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

  • Gi/o GPCRs drive the formation of actin-rich tunneling nanotubes in cancer cells via a Gβγ/PKCα/FARP1/Cdc42 axis

    Oncology and Immunology Gi/o GPCRs drive the formation of actin-rich tunneling nanotubes in cancer cells Using a model of human adrenocortical cancer cells, here we established that activation of the GPCR OXER1 acid (5-oxo-ETE), leads to the formation of filopodia-like elongated projections connecting adjacent cells Kazanietz Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

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