GPCR News < GPCRs in Oncology and Immunology G protein coupled receptor transcripts in human immune cells We have used the method to profile GPCR transcripts in white blood cells (WBCs)-B, CD4, CD8, NK, and dendritic cells; monocytes, and macrophage-like monocytes treated with granulocyte-macrophage colony-stimulating factor-as well as platelets. On average, the white cells studied expressed 160 receptor mRNAs (range, 123-206).

News < GPCRs in Oncology and Immunology The orphan G protein-coupled receptor 141 expressed in myeloid cells High GPR141 messenger RNA levels were expressed in myeloid-lineage cells, including neutrophils (CD11b Gpr141 -/- mice, which we independently generated, displayed almost no abnormalities in myeloid cell myelin oligodendrocyte glycoprotein 35-55-specific T cells. , experimental autoimmune encephalomyelitis , monocytes , myeloid cells .

News < GPCRs in Oncology and Immunology Purinergic GPCR-integrin interactions drive pancreatic cancer cell P2RY2 presents as the purinergic gene with the strongest association with hypoxia, the highest cancer cell-specific receptor-integrin interactions were required for effective downstream signalling, leading to cancer cell Hemant M Kocher , Sabrina Simoncelli , Peter J McCormick , Richard Philip Grose Tags cancer biology , cell Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call for GPCR

< GPCR News < GPCRs in Oncology and Immunology The EBI2 receptor is coexpressed with CCR5 in CD4+ T cells Methods: We identified GPCRs expressed in primary CD4+CCR5+ T cells by multi-RT-qPCR. Cell lines expressing EBI2 were established by transduction with HIV vectors. The amount of HIV reverse transcripts was similar in cells expressing or not EBI2. Conclusions: EBI2 expression in CD4+CCR5+ cells boosts HIV-1 R5 productive infection.

in Oncology and Immunology Vasoactive intestinal peptide receptor 2 signaling promotes breast cancer cell cell lines, promoted cell proliferation. proliferation in VIPR2-overexpressing MCF-7 and MDA-MB-231 cells. was greater than that in control cells, suggesting the increased PKA activity. at the same level as observed in EGFP-expressing cells treated with U0126.

< GPCR News < GPCRs in Oncology and Immunology NPFF stimulates human ovarian cancer cell invasion by The TaqMan probe-based RT-qPCR showed that NPFF and NPFFR2 were expressed in three human EOC cells, CaOV3 In comparison, NPFF and NPFFR2 expression levels were higher in SKOV3 cells than in CaOV3 or OVCAR3 cells Treatment of SKOV3 cells with NPFF did not affect cell viability and proliferation but stimulated cell This study provides evidence that NPFF stimulates EOC cell invasion by upregulating MMP-9 expression

< GPCR News < GPCRs in Oncology and Immunology The GPCR-Gαs-PKA signaling axis promotes T cell dysfunction Here, we cross-integrated large singe-cell RNA-sequencing datasets from CD8+ T cells covering 19 distinct cancer types and identified an enrichment of Gαs-coupled GPCRs on exhausted CD8+ T cells. These include EP2, EP4, A2AR, β1AR and β2AR, all of which promote T cell dysfunction. Gαs-DREADD to activate CD8-restricted Gαs signaling and show that a Gαs-PKA signaling axis promotes CD8+ T cell

< GPCR News < GPCRs in Oncology and Immunology Molecular characterization of breast cancer cell pools Methods: We disrupted the PR gene (PGR) in ERα-positive/PR-positive T-47D cells using the CRISPR/Cas9 to control T-47D cells. We analyzed the gene expression profiles of PR-low and control T-47D cells in the absence of hormone More than 6000 genes were DE in control T-47D cells upon stimulation with P4 or P4 plus E2.

and Immunology Opposite Effects of Src Family Kinases on YAP and ERK Activation in Pancreatic Cancer Cells IGF-1 receptors and G protein-coupled (GPCR) signaling systems leading to mitogenic signaling in PDAC cells agonist neurotensin induced rapid activation of Src family of tyrosine kinases (SFK) within PANC-1 cells by FAK phosphorylation at Tyr576/577 and Tyr861, sensitive biomarkers of SFK activity within intact cells and suppressed the growth of Mia PaCa-2 cells xenografted into the flank of nude mice.

constitutes GPCR-mediated phospholipase C (PLC) signaling and is essential for neutrophil polarization and migration In response to a chemoattractant stimulation, cells lacking PLCγ2 (plcg2kd) displayed altered dynamics phosphorylation and cofilin activation; impaired dynamics of actin polymerization; and consequently, defects in cell polarization and migration during chemotaxis. Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call for GPCR

< GPCR News < GPCRs in Oncology and Immunology RGS20 promotes non-small cell lung carcinoma proliferation CCK8 and cell cloning were conducted to determine the proliferation ability of H1299 and Anip973 cells Further, RGS20 accelerated cell proliferation by increasing autophagy. in RGS20 knock-down cells. Conclusion: Our findings indicate that RGS20 drives NSCLC cell proliferation by triggering autophagy

< GPCR News < GPCRs in Oncology and Immunology Single cell G-protein coupled receptor profiling of Transcription Key results: Transcription factor 21 (Tcf21)+ cells that expressed 2 or 3 of Postn, Acta2 and Pdgfra Tcf21+ α-smooth muscle actin (α-SMA)+ interstitial cells accumulated in the kidneys of mice with UUO TCF21, ADGRA2, S1PR3 and ADORA2A/2B were each detectable in α-SMA+ interstitial cells in human kidneys Study of GPCRs expressed by these cells may identify new opportunities for CKD therapeutics. " Authors

2022 Abstract The rheumatoid arthritis (RA) inflammatory process occurs in the joints where immune cells as to perform a functional migration assay in response to GPR15L. synovial fluid and peripheral blood were analyzed for CD45+CD3+CD4+GPR15+ and CD45+CD3+CD8+GPR15+ T cell Migration assays were performed using PBMCs isolated from these individuals in response to the synthetic GPR15 and GPR15L are present in the synovial tissue of RA patients and GPR15L promotes migration of PBMCs

induces IFN-γ but suppresses IL-2 production by inhibiting activation of pAKT pathways in primary T cells signaling molecules and the phosphatidylinositol, Ras, MAPK, and PI3 kinase pathways, leading to T cell This may explain the inhibitory impact on IL-2 production in hM3Dq/β1T cells. that activating the pAKT pathway is critical for IL-2 production in T cells." Tags GPCR; T cells; muscarinic receptor; signaling.

Bispecific antibodies targeting BCMA or GPRC5D are highly effective in relapsed myeloma after CAR T-cell December 5, 2024 Abstract "Despite the astonishing outcomes after chimeric antigen receptor (CAR) T-cell There are only limited data available on salvage therapies following relapse after BCMA-directed CAR T-cell Here, we analyzed outcomes of post-CAR T-cell therapy relapse and impact of different salvage strategies The median time to relapse after CAR T-cell therapy was 5 months, 53% had the extramedullary disease

Metallo-protease Peptidase M84 from Bacillusaltitudinis induces ROS-dependent apoptosis in ovarian cancer cells This metallo-protease had no discernible impact on normal cell survival, but it specifically induced apoptosis in ovarian cancer cells. PAR-1, a GPCR which is reported to be overexpressed in ovarian cancer cells, was identified as a target This evoked apoptotic death of the ovarian cancer cells through the intrinsic route.

Oncology and Immunology Comparative analysis of the occupancy of Histone H3 Lysine 4 methylation in the cells 28, 2023 Abstract "In this current study, we have compared our H3K4me3 Chip-Sequencing data in PC3 cells in response to 6h and 24h TGFβ stimulation with the IFNγ stimulated/unstimulated HeLa S3 cells Since modification changes in response to TGFβ and IFNγ and compare them to explore the genes common to both as well Noopur Thakur Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

in Oncology and Immunology Autocrine proteinase-activated receptor signaling in PC3 prostate cancer cells Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call for GPCR

Oncology and Immunology Enterococcus-derived tyramine hijacks α2A-adrenergic receptor in intestinal stem cells disease (IBD) is characterized by dysbiosis of the gut microbiota and dysfunction of intestinal stem cells Using an engineered tyrDC-deficient Enterococcus faecalis strain and intestinal epithelial cell-specific adrenergic receptor Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

Immunology Comparative Analysis of the GNAI Family Genes in Glioblastoma through Transcriptomics and Single-Cell regulation of actin cytoskeleton organization by the kinase effectors of Rho GTPases" and "Immune response B cell A single-cell analysis was used to assess GNAI3 expression in GBM. Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call for GPCR

< GPCR News < GPCRs in Oncology and Immunology A GPCR checkpoint drives CD8+ T cell dysfunction and immunotherapy Authors listed Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

< GPCR News < GPCRs in Oncology and Immunology miR-19a may function as a biomarker of oral squamous cell Using a transcriptomic analysis of over 37,000 nuclei, we identified twelve distinct clusters of cells corresponding to temperature-sensing arista neurons, humidity-sensing sacculus neurons, and support cells We found that each cell type could be characterized by a unique expression profile of ion channels, GPCR signaling molecules, synaptic vesicle cycle proteins, and cell adhesion molecules.

modification that supports membrane interactions of proteins involved in various cellular processes, including migration We examined the prenylation of carboxy-terminus (Ct) mutated Gγ in cells exposed to Fluvastatin and prenyl monitored the subcellular localization of fluorescently tagged Gγ subunits and their mutants using live-cell Given the cell and tissue-specific expression of different Gγ subtypes, our findings indicate a plausible mechanism allowing for statins to differentially perturb heterotrimeric G protein signaling in cells

Immunology [1,2,4]Triazolo[1,5-c]pyrimidines as Tools to Investigate A3 Adenosine Receptors in Cancer Cell Compound 20 has been tested on both A3 adenosine receptor positive cancer cell lines (CHO-A3AR transfected , THP1 and HCCT26) and in A3 negative cancer cell lines, showing no effect on the latter and a proliferative antagonists Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

Docking of identified hits to RGS2 as well as other RGS structures was used to screen the hits for potent Whole cell assays showed the top 10 ranking compounds, AJ-1-AJ-10, to inhibit RGS2-Gαq interactions. All 10 compounds inhibited the growth of several RGS2 expressing cancers in cell culture assays. In addition, AJ-3 inhibited the migration of LNCaP prostate cancer cells in wound healing assays. M Mahfouz Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

G protein signaling protein 6 alleviates acute lung injury by inhibiting inflammation and promoting cell Organoid culture was used to assess the stemness and self-renewal capacity of alveolar epithelial type II cells in macrophages and decreased apoptosis in epithelial cells. a protective role in ALI not only in early inflammatory responses but also in endogenous lung stem cell Zhou , Linlin Wang , Yu Yan , Jun She , Lin Tong , Yuanlin Song Tags Acute lung injury , Apoptosis , Cell-renewal

long non-coding RNA (LncRNA) PAXIP1 antisense RNA 1 (PAXIP1-AS1) was found to promote proliferation, migration , EMT, and apoptosis of ovarian cancer (OC) cells in OC cell lines, but the relationship between PAXIP1 QRT-PCR was used to validate the expression of PAXIP1-AS1 in OC cell lines. PAXIP1-AS1 was significantly downregulated in OC cell lines compared with IOSE29 cell line. , Hui Liu Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

Immunology The pyruvate-GPR31 axis promotes transepithelial dendrite formation in human intestinal dendritic cells Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call for GPCR