< GPCR News < GPCRs in Oncology and Immunology The GPCR-Gαs-PKA signaling axis promotes T cell dysfunction Here, we cross-integrated large singe-cell RNA-sequencing datasets from CD8+ T cells covering 19 distinct cancer types and identified an enrichment of Gαs-coupled GPCRs on exhausted CD8+ T cells. These include EP2, EP4, A2AR, β1AR and β2AR, all of which promote T cell dysfunction. Gαs-DREADD to activate CD8-restricted Gαs signaling and show that a Gαs-PKA signaling axis promotes CD8+ T cell

in Oncology and Immunology LPA1-mediated inhibition of CXCR4 attenuates CXCL12-induced signaling and cell Results: We observed that CXCR4 forms heteromers with LPA1 in recombinant HEK293A cells and the human breast cancer cell line MDA-MB-231. MDA-MB-231 cells but not in LPA1-deficient cells. have been evidenced across various cell lines.

< GPCR News < GPCRs in Oncology and Immunology RGS20 promotes non-small cell lung carcinoma proliferation Abstract " Background: Novel therapeutic targets are urgently needed for treating drug-resistant non-small cell CCK8 and cell cloning were conducted to determine the proliferation ability of H1299 and Anip973 cells Further, RGS20 accelerated cell proliferation by increasing autophagy. in RGS20 knock-down cells.

< GPCR News < GPCRs in Oncology and Immunology GPR4 in the pH-dependent migration of melanoma cells in pH-gradient) is a well-known driver of tumor progression and metastasis. In this study, we investigated the pH-dependent migration of GPR4 wildtype/overexpressing SK-Mel-28 cells Migration of GPR4 overexpressing SK-Mel-28 cells was enhanced in a range of pH 6.5 - pH 7.5 as compared Results indicate that GPR4 is involved in the migration of melanoma cells, especially in the tumor periphery

< GPCR News < GPCRs in Oncology and Immunology Single cell G-protein coupled receptor profiling of Transcription Key results: Transcription factor 21 (Tcf21)+ cells that expressed 2 or 3 of Postn, Acta2 and Pdgfra Tcf21+ α-smooth muscle actin (α-SMA)+ interstitial cells accumulated in the kidneys of mice with UUO TCF21, ADGRA2, S1PR3 and ADORA2A/2B were each detectable in α-SMA+ interstitial cells in human kidneys Study of GPCRs expressed by these cells may identify new opportunities for CKD therapeutics. " Authors

expression of GPR50 promotes the proliferation, migration and autophagy of hepatocellular carcinoma cells was analyzed in HCC patients (gene expression omnibus database (GEO) (GSE45436)) and detected in HCC cell 7919, and the results showed that GPR50 was significantly up-regulated in HCC patients and CBRH-7919 cell Gpr50 cDNA was transfected into HCC cell line CBRH-7919, and we found that Gpr50 promoted the proliferation Cuifang Chang Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

and Immunology Dynamic Phosphoproteomics of BRS3 Activation Reveals the Hippo Signaling Pathway for Cell The lung cancer cell line H1299-BRS3 was treated with MK-5046, an agonist of BRS3, for different durations Harvested cellular proteins were digested and phosphopeptides were enriched by immobilized titanium ( dephosphorylation and nucleus localization of the Yes-associated protein (YAP), and its association with cell Our data collectively demonstrate that BRS3 activation contributes to cell migration through downregulation

< GPCR News < GPCRs in Oncology and Immunology TIPE proteins control directed migration of human T cells human TIPE family members, TNFAIP8 and TIPE2 were essential for directed migration of human CD4+ T cells T Cells deficient in both of these proteins completely lost their directionality. bisphosphate (PIP2) and phosphatidylinositol 3,4,5-triphosphate (PIP3) to spatiotemporally control immune cell Collectively, our work describes a new mechanistic paradigm for how human T cells integrate GPCR and

News < GPCRs in Oncology and Immunology Purinergic GPCR-integrin interactions drive pancreatic cancer cell P2RY2 presents as the purinergic gene with the strongest association with hypoxia, the highest cancer cell-specific receptor-integrin interactions were required for effective downstream signalling, leading to cancer cell Hemant M Kocher , Sabrina Simoncelli , Peter J McCormick , Richard Philip Grose Tags cancer biology , cell Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call for GPCR

Oncology and Immunology Comparative analysis of the occupancy of Histone H3 Lysine 4 methylation in the cells 28, 2023 Abstract "In this current study, we have compared our H3K4me3 Chip-Sequencing data in PC3 cells in response to 6h and 24h TGFβ stimulation with the IFNγ stimulated/unstimulated HeLa S3 cells Since modification changes in response to TGFβ and IFNγ and compare them to explore the genes common to both as well Noopur Thakur Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

kinase (MAGUK) scaffold protein, and protein kinase A anchoring protein (AKAP) 5 in MCF7 breast cancer cells MCF7 breast cancer cells express GPR30, β1AR, MAGUKs, and AKAP5 in the plasma membrane, and co-immunoprecipitation Furthermore, expression of GPR30 in MCF7 cells constitutively and PDZ-dependently inhibits β1AR-mediated These results argue that GPR30 and β1AR form a PDZ-independent complex in MCF7 cells through which GPR30 adrenergic receptor Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

G protein signaling protein 6 alleviates acute lung injury by inhibiting inflammation and promoting cell Organoid culture was used to assess the stemness and self-renewal capacity of alveolar epithelial type II cells in macrophages and decreased apoptosis in epithelial cells. a protective role in ALI not only in early inflammatory responses but also in endogenous lung stem cell Zhou , Linlin Wang , Yu Yan , Jun She , Lin Tong , Yuanlin Song Tags Acute lung injury , Apoptosis , Cell-renewal

< GPCR News < GPCRs in Oncology and Immunology A GPCR checkpoint drives CD8+ T cell dysfunction and immunotherapy Authors listed Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

Immunology [1,2,4]Triazolo[1,5-c]pyrimidines as Tools to Investigate A3 Adenosine Receptors in Cancer Cell Compound 20 has been tested on both A3 adenosine receptor positive cancer cell lines (CHO-A3AR transfected , THP1 and HCCT26) and in A3 negative cancer cell lines, showing no effect on the latter and a proliferative antagonists Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

Oncology and Immunology Enterococcus-derived tyramine hijacks α2A-adrenergic receptor in intestinal stem cells disease (IBD) is characterized by dysbiosis of the gut microbiota and dysfunction of intestinal stem cells Using an engineered tyrDC-deficient Enterococcus faecalis strain and intestinal epithelial cell-specific adrenergic receptor Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

Oncology and Immunology Gi/o GPCRs drive the formation of actin-rich tunneling nanotubes in cancer cells Using a model of human adrenocortical cancer cells, here we established that activation of the GPCR OXER1 acid (5-oxo-ETE), leads to the formation of filopodia-like elongated projections connecting adjacent cells Kazanietz Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

of the nematode-trapping fungus Arthrobotrys flagrans activates mitochondria and reprograms fungal cells GPCR, GprC, at the plasma membrane and together with the G-protein alpha subunit GasA, reprograms the cell Reinhard Fischer Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

Immunology Comparative Analysis of the GNAI Family Genes in Glioblastoma through Transcriptomics and Single-Cell regulation of actin cytoskeleton organization by the kinase effectors of Rho GTPases" and "Immune response B cell A single-cell analysis was used to assess GNAI3 expression in GBM. Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call for GPCR

Methods: GPR81 was stably knocked down (KD) in MCF-7 human breast cancer cells which were subjected to RNA-seq analysis, 3D growth, in situ- and immunofluorescence analyses, and cell viability- and motility Key findings were additionally studied in other breast cancer cell lines and in mammary epithelial cells Single cell in situ analysis of MCF-7 cells revealed that several GPR81-regulated genes were upregulated in the same cell clusters.

Abstract "The CXCL16-CXCR6 axis is crucial for regulating the persistence of CD8 tissue-resident memory T cells CXCR6 deficiency lowers TRM cell numbers in the lungs and depletes ILC3s in the lamina propria, impairing The unique DRF motif of CXCR6 facilitates leukocyte adhesion by interacting with cell surface-expressed Notably, single-cell RNA sequencing of the lung shows a drop in TRM cells in species with CXCR6 loss, with implications for immunity against viral diseases and vaccines inducing CD8 TRM cells."

Here we reported the first comprehensive proteogenomic characterization of natural killer/T-cell lymphoma Single-cell transcriptome further delineated the tumor microenvironment as immune-inflamed, -deficient Enhanced expression of chemokine receptor-1 (CCR1) on malignant and immunosuppressive cells modulated Therapeutic targeting CCR1 showed promising efficacy with EBV eradication, T-cell activation, and lymphoma cell killing in NKTCL organoid.

GPR37 gene expression and the clinicopathological factors, patient prognosis, tumor-infiltrating immune cell Besides, the "ssGSEA" algorithm was conducted to estimate immune cells infiltration abundance, with ' differentiation, negative regulation of T cell receptor signaling pathway, neuroactive ligand receptor , CD8 T cell, eosinophils, macrophages, neutrophils, NK CD56dim cells, NK cells, plasmacytoid DCs (pDCs ), T helper cells and T effector memory (Tem) cells.

Low extracellular pH confers radioresistant tumors to glial cells. inhibitor of GPR68 named Ogremorphin (OGM) to induce the iron mediated cell death pathway: ferroptosis Next, A GPI-anchored pH reporter, pHluorin2, was stably expressed in U87 glioblastoma cells to probe Cell survival assays, via nuclei counting and cell titer glo, were used to demonstrate sensitivity to To determine GPR68 inhibition's mechanism of cell death we use DAVID pathway analysis of RNAseq.

Herein we propose a model for immune conditioning, whereby metabolites such as butyrate affect immune cells Deficiency of SCFA would lead to pro-inflammatory immune cell skewing through insufficient G-protein Such pro-inflammatory immune cells may travel to tissues such as the brain, the lung, the kidney etc This model helps explain how the gut microbiome may be affecting peripheral immune cells, and consequently fatty acids Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

Chemokines are chemotactic cytokines whose canonical functions govern movement of receptor expressing cells chemokines play paradoxical roles in both the directed emigration of metastatic, receptor-expressing cancer cells out of the tumor as well as immigration of tumor infiltrating immune cells that culminate in a tumor In this article we review current literature on the diversity of chemokine ligands and their cellular Authors Donovan Drouillard, Brian T Craig, Michael B Dwinell Tags Chemokine receptor; cell migration;

previously showed that ColI(2.3)+/Rs1+ mice, in which Gs-GPCR signaling was hyper-activated in osteoblastic cell signaling pathway has been implicated in the pathogenesis of FD-like bone, but the specific Wnts and which cells Single-cell RNA sequencing on long-bone stromal cells of 9-wk-old male ColI(2.3)+/Rs1+ mice and littermate controls showed that fibroblastic stromal cells in ColI(2.3)+/Rs1+ mice were expanded. cells.

S2 cells. Furthermore, treatment of S2 cells with GBP2 enhanced GBP1 expression levels, but GBP1 did not affect GBP2-induced enhancement of GBP1 expression was not observed in Mthl10 knockdown cells. Enhancement of GBP2 expression was observed in both Drosophila larvae and S2 cells under heat stress Finally, Ca2+ mobilization assay in GCaMP3-expressing S2 cells demonstrated that GBP2 mobilizes Ca2+

and hypoxia on migration, proliferation, invasion, and signaling in 2D and 3D models of breast cancer cell Antagonizing NPY1R and/or NPY5R in hypoxia compared to normoxia more greatly reduced MAPK signaling, cell proliferation, cell migration and invasion, and spheroid growth and invasion. The estrogen receptor positive MCF7 cells were significantly less invasive in 3D spheres when NPY5R was There were some discrepancies in the responses of each cell line to the isoform-specific antagonists