, we demonstrate that GBP2 replicates the GBP1-mediated cellular immune response from Drosophila S2 cells Furthermore, treatment of S2 cells with GBP2 enhanced GBP1 expression levels, but GBP1 did not affect GBP2-induced enhancement of GBP1 expression was not observed in Mthl10 knockdown cells. Enhancement of GBP2 expression was observed in both Drosophila larvae and S2 cells under heat stress Finally, Ca2+ mobilization assay in GCaMP3-expressing S2 cells demonstrated that GBP2 mobilizes Ca2+

Planar cell polarity (PCP) proteins contribute to tumour growth and invasion. protein isoforms differentially regulate tissue adhesiveness by influencing the stability/plasticity of cell-cell and cell-matrix contacts. assays in vitro and in vivo, whether CELSR1 protein isoforms differentially influence the stability of cell-cell However, how the ECR communicates with the seven-pass transmembrane domain (7TM) remains elusive, because

GPCR Community Presentation Breaking Barriers: My Journey from Mexico to the Heart of the Dr. focuses on understanding the molecular mechanisms of adhesion GPCRs (aGPCRs) in lymphatic endothelial cells

and hypoxia on migration, proliferation, invasion, and signaling in 2D and 3D models of breast cancer cell Antagonizing NPY1R and/or NPY5R in hypoxia compared to normoxia more greatly reduced MAPK signaling, cell proliferation, cell migration and invasion, and spheroid growth and invasion. The estrogen receptor positive MCF7 cells were significantly less invasive in 3D spheres when NPY5R was There were some discrepancies in the responses of each cell line to the isoform-specific antagonists

Herein we propose a model for immune conditioning, whereby metabolites such as butyrate affect immune cells Deficiency of SCFA would lead to pro-inflammatory immune cell skewing through insufficient G-protein Such pro-inflammatory immune cells may travel to tissues such as the brain, the lung, the kidney etc This model helps explain how the gut microbiome may be affecting peripheral immune cells, and consequently fatty acids Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

Chemokines are chemotactic cytokines whose canonical functions govern movement of receptor expressing cells chemokines play paradoxical roles in both the directed emigration of metastatic, receptor-expressing cancer cells out of the tumor as well as immigration of tumor infiltrating immune cells that culminate in a tumor Authors Donovan Drouillard, Brian T Craig, Michael B Dwinell Tags Chemokine receptor; cell migration; Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call for GPCR

Increasing evidence reveals the heightened expression of olfactory receptors in tumorous tissues and cells Olfactory receptors have demonstrated influence over tumor cell proliferation and metastasis, establishing Guo-Tai Wang Tags G Protein-coupled receptors , Olfactory receptors , Therapeutic targeting , Tumor cell Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call for GPCR

transmitting signals inside the body, which is necessary for controlling the life activities of all cells , including tumor cells [1]. ATP functions as a mighty damage-linked molecular pattern when released outside the cell, accumulating protein-coupled receptors (GPCR) (P2Y) interact with ATP and other nucleotides, influencing diverse immune cell microenvironment (TME) Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

protein-coupled receptors (GPCRs), enhancing proliferation, adhesion, and migration in many types of cancer cells Our group previously reported that LPA induces production of CCN1 protein in human prostate cancer cell In these cells, the mitogenic activity of LPA is mediated by LPA Receptor 1 (LPAR1), a GPCR. In some model systems, CCN1 and CCN2 play key roles in LPA/S1P-induced cell migration and proliferation Kathryn E Meier Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

Mesenchymal cells play key roles in numerous developmental processes. subunits Gαq and Gα11 (Gαq/11) transmit mechanical and chemical signals to activate TGFβ in epithelial cells exhibited abnormal alveolar development, with suppressed myofibroblast differentiation, altered mesenchymal cell synthetic function, and reduced lung TGFβ2 deposition, as well as kidney abnormalities. undescribed mechanism of cyclical stretch-induced Gαq/11-dependent TGFβ2 signalling in mesenchymal cells

., PNAS, 2014) in breast cancer pathogenesis, using mouse models, three-dimensional cell culture and Mike's Research on Cell Polarity and GPCRs in Cancer Mike shared his research on cell polarity and its He also discussed the impact of disrupting histone processing on rapidly proliferating cells, such as cancer cells, and suggested a therapeutic index for a drug called JTE-6.7. He also discussed his experiments and discoveries about GPR161 in mammary epithelial cells, the effect

Depletion of GPR37 significantly reduced CRC tumor cell growth both in vitro and in vivo. Further tests showed that GPR37 protects cancer cells from ferroptosis by upregulating SCD1 expression Mechanistic studies have shown that GPR37 modulates lipid metabolism in tumor cells by promoting SCD1 SCD1 , p38 Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

of 10 kDa (IP-10/CXCL10) is a dual-function CXC chemokine that coordinates chemotaxis of activated T cells and natural killer (NK) cells via interaction with its G protein-coupled receptor (GPCR), CXC chemokine CXCL10(1-77) using surface plasmon resonance for glycosaminoglycan (GAG) binding affinity, assays for cell migration, second messenger signaling downstream of CXCR3, and flow cytometry of CHO cells and primary human T lymphocytes and endothelial cells.

Th17 cells express the CCR6 receptor and inflammatory cytokines, including IL-17, IL-21 and IL-22, which The CCL20/CCR6 mechanism plays a crucial role in the recruitment of these pro-inflammatory cells to local Tags CCR6 , GPCRs , Th17 cells , antibody , immune system , inflammation , therapy . Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call for GPCR

2024 Abstract "Efficient induction of humoral immune responses depends on orchestrated migration of B cells recruits a specific GRK to chemoattractant receptors and plays an important role in the control of B cell Authors Taiichiro Shirai , Akiko Nakai , Kazuhiro Suzuki Tags B cell migration , COMMD3/8 complex , GRK Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call for GPCR

Its high expression on the surface of multiple myeloma cells has rendered it an attractive target for therapeutic interventions, including monoclonal antibodies, CAR-T cells, and T-cell engagers. Liu, Fei Xu Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

We examined the prenylation of carboxy-terminus (Ct) mutated Gγ in cells exposed to Fluvastatin and prenyl monitored the subcellular localization of fluorescently tagged Gγ subunits and their mutants using live-cell Given the cell and tissue-specific expression of different Gγ subtypes, our findings indicate a plausible mechanism allowing for statins to differentially perturb heterotrimeric G protein signaling in cells prenyltransferases , statin Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

human protein atlas database, we inferred the most predominant GPCR-mediated microbial metabolite-human cell permeability of the small-molecules we elucidated their molecular interactions with specific human cell receptors, particularly expressed on human intestinal epithelial cells, immune cells and the nervous Metabolites Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

polarity (PCP) pathway, which establishes and maintains cell and tissue polarity along the tissue plane We initially tested the ADC using in vitro WST-8 viability assays in human GBM cell lines and cell types We observed significantly lower LD50 values in patient-derived and U87 GBM cell cultures vs. CD97-lacking cells. We also found significantly lower LD50 values when treating human GBM cells with the ADC (0.6788 nM),

Whereas it is well established that single-cell migration is often guided by gradients of chemokines Upon exposure to the CCR7 ligand CCL19, dendritic cells (DCs) effectively internalize the receptor and patterns to the size and geometry of the environment, and provide a guidance cue for other comigrating cells Michael Sixt Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

PAXIP1-AS1) was found to promote proliferation, migration, EMT, and apoptosis of ovarian cancer (OC) cells in OC cell lines, but the relationship between PAXIP1-AS1 expression and clinical characteristics, prognosis QRT-PCR was used to validate the expression of PAXIP1-AS1 in OC cell lines. PAXIP1-AS1 was significantly downregulated in OC cell lines compared with IOSE29 cell line. , Hui Liu Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

FRET and single-molecule microscopy, which allow them to directly observe signalling events in living cells Davide’s work has been published in prestigious scientific journals such as Nature, Cell, New England Journal of Medicine, Journal of Clinical Investigation, PLoS Biology, PNAS, Nature Communications and

heterotrimeric G-proteins (Gαβγ) by G-protein-coupled receptors (GPCRs) is a quintessential mechanism of cell disrupted their engagement with GIV/Girdin, thereby blocking noncanonical G-protein signaling in tumor cells and inhibiting proinvasive traits of metastatic cancer cells. Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call for GPCR

Here, we showed that US28 increased the malignancy of U251 glioblastoma cells by enhancing signaling Enhanced S1P signaling promoted glioblastoma cell proliferation and survival by activating the kinases US28 also activated the S1P signaling axis in HCMV-infected cells. Authors Nick D Bergkamp , Jeffrey R van Senten , Hendrik J Brink , Maarten P Bebelman , Jelle van den Martine J Smit Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

MM) is a complex hematological malignancy characterized by abnormal antibody production from plasma cells G-protein-coupled receptor class C group 5 member D (GPRC5D), an orphan GPCR predominantly expressed in MM cells Talquetamab, a Food and Drug Administration-approved T-cell-directing bispecific antibody developed for talquetamab Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

Docking of identified hits to RGS2 as well as other RGS structures was used to screen the hits for potent Whole cell assays showed the top 10 ranking compounds, AJ-1-AJ-10, to inhibit RGS2-Gαq interactions. All 10 compounds inhibited the growth of several RGS2 expressing cancers in cell culture assays. In addition, AJ-3 inhibited the migration of LNCaP prostate cancer cells in wound healing assays. M Mahfouz Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

Furthermore, fibroblasts abundantly expressed Gpr176 compared to alveolar epithelial cells, endothelial cells, and macrophages in the fibrotic lung. GPR176 expression was unaffected by TGFβ1 stimulation in rat renal fibroblast NRK-49 cells, whereas knockdown of Gpr176 by siRNA reduced TGFβ1-induced expression of αSMA, fibronectin, and collagen as well as Smad2 GPCR , TGFβ1 Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

treating patients suffering from lung cancer is to target surface receptors overexpressed on tumor cells cancers, such as prostate and ovarian cancer, facilitating the invasive and metastatic capacity of tumor cells The expression levels of KLKs in this neoplasm are modulated by the secretome of the different cell types SARS-CoV-2 Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call