Randy’s lab has a special interest in studying disease-associated mutations to human GPCRs that perturb

Abstract "Li-Fraumeni syndrome (LFS) is a rare cancer predisposition syndrome caused by a germline mutation Glioblastoma (GBM) is the most prevalent central nervous system tumor in LFS, with TP53 mutations detected that ADGRB3 functions as a tumor suppressor in the brain, and its silencing, in the context of p53 mutation Isolated GBM stem cells were collected for further genomic analyses and to test whether overexpression flexibility of GAIN flaps affects aGPCR function under native conditions, we strategically inserted specific mutations

Furthermore, conservation analysis indicates that wild type ADGRB2 and ADGRB3 are GPS cleavage-incompetent Mutational experiments on residues of interest showed that restoring the Phe-His interaction in the uncleaving

We analyzed the gene expression profiles of PR-low and control T-47D cells in the absence of hormone genes between experimental groups were characterized based on RNA-seq and Gene Ontology (GO) enrichment analyses GO analysis revealed seven significantly enriched biological processes affected by PR and associated

dysplasia (FD) of the bone is characterized by fibrotic, expansile bone lesions caused by activating mutations

Methods: Bioinformatics analysis was performed to assess the correlation between EGFR and SSTR2 based Results: The bioinformatics analysis and IHC showed a positive correlation between SSTR2 and EGFR in Cox multivariate analysis identified SSTR2 and EGFR as independent poor predictors of PFS.

Real-time PCR analysis, bioinformatic analysis and luciferase assays were performed to establish a potential Moreover, in silico analysis confirmed a potential binding site of miR-19a on the 3'UTR of GRK6 mRNA,

Kruskal-Wallis test, Wilcoxon sign-rank test, logistic regression, Kaplan-Meier method, Cox regression analysis , Gene set enrichment analysis (GSEA), and immuno-infiltration analysis were used to evaluate the relationship

2012 from the University of Oxford, where her research focused on the cellular mechanisms by which mutations

GFs under 1 mg/L LPS-induced GFs inflammatory state were evaluated by RT-qPCR and Western blotting analysis Western blotting analysis and immunofluorescence staining were used to further investigate the activation The results of RT-qPCR and Western blotting analysis showed that siGPRC5A (0.27±0.03, 0.71±0.00) suppressed Western blotting analysis showed that the levels of p65 and IκBα protein phosphorylation in the LPS group

immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) for label-free quantification (LFQ) analysis Data analysis revealed that 27 phosphopeptides corresponding to six proteins were involved in the Hippo

Secondary analysis evaluated risk factors for late respiratory infections using univariate and multivariable On multivariable analysis, hypogammaglobulinemia (hazard ratio [HR], 6.06; P = .044) and diagnosis of

Statistical analysis using GEO datasets was conducted. showed weak antagonistic activity on M3, which was confirmed to be a disease target by statistical analysis

After 2-24 h, brains were collected for the analysis of gene expression by RT-PCR or protein expression with LPS (100 ng/ml) followed by 10 nM synaptamide for 2 h for gene expression and 12 h for protein analysis

Steering Committee of the San Diego Supercomputer Center, and provided consultation for genetic sequence analysis Bioinformatics Core Facility Manager, where they managed a technical support team providing scientific data analysis and database development services, contributed analytic support resulting in authorship of numerous point of contact for customers assessing features of software products for drug discovery and genomic analysis

Outside of the lab, I am an avid bike racer, an occasional cycling coach, and I enjoy spending time with

engineering and has been working in machine learning for nearly 10 years, applying it to antibody generation, analysis

findings by emphasizing the structural and evolutionary significance of TACR3 and provided an in-depth analysis

GPCRdb database where ~4,000 researchers each month retrieve reference data and access online tools for analysis

Our structural analysis reveals that the GPRC5D presents a close resemblance to the typical Class C GPCRs

CRISPR/Cas9 targeting of PAR1 and PAR2 combined with microarray analysis revealed genes that are regulated

To detect the role and the regulation mechanism of GPR50 in HCC, GPR50 expression was analyzed in HCC mechanism of GPR50 in HCC was detected by isobaric tags for relative and absolute quantification (iTRAQ) analysis

, cell-cell communication , drug repurposing , personalized medicine , signaling network , survival analysis

Transcriptomic analysis of Ltb4r2-/- and WT macrophages suggested a role for BLT2 in macrophage migration

Subsequent signaling analysis reveals a strict requirement of phospholipase C β3 (PLCβ3) and its downstream

Preliminary analysis of the specificity/selectivity of one of the most effective hit compounds indicated

In gene expression analysis of mouse lungs with induced fibrosis, eight GPCRs were identified, showing

The expression of GPR15 and c10orf99/gpr15l mRNA was analyzed by RT-qPCR. Samples of synovial fluid and peripheral blood were analyzed for CD45+CD3+CD4+GPR15+ and CD45+CD3+CD8 Statistical analysis included Kruskal-Wallis test, T-test, or Mann-Whitney U test, according to data