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271 items found for "immune cells"
- Ep 126 with Dr. Françoise Bachelerie
activation/function of CXCR4-ACKR3 (CXCR7) receptors of the CXCL12 chemokine, key effectors of the immune
- Molecular docking and dynamics simulation studies uncover the host-pathogen protein-protein interactions in Penaeus vannamei and Vibrio parahaemolyticus
Hence, this study was conducted to identify the interaction sites and binding affinity between several immune-related Afiqah-Aleng Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call
- Interaction with the cell adhesion molecule NEGR1 affects mGluR5 cell signalling
Registration Logo Contest Committee Sponsors GPCR Retreat Program < Back to schedule Interaction with the cell adhesion molecule NEGR1 affects mGluR5 cell signalling Date & Time Friday, November 3rd / 1:30 PM Abstract Ribeiro has supervised eleven M.Sc. and six Ph.D. students, as well as five post-doctorate fellows. Nowadays, her research group comprises four undergraduates, two M.Sc., and six Ph.D. students, as well These drugs were shown to be very effective to rescue the cell death observed in a mouse model of Huntington
- Receptor autoantibodies: Associations with cardiac markers, histology, and function in human non-ischaemic heart failure
Here, we use validated and certified immune assays presenting native receptors as binding targets. Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call for GPCR
- The β2-adrenergic receptor associates with CXCR4 multimers in human cancer cells
Oncology and Immunology The β2-adrenergic receptor associates with CXCR4 multimers in human cancer cells PIE-FCCS can resolve membrane protein density, diffusion, and multimerization state in live cells at into multimeric complexes larger than dimers in MDA-MB-231 human breast cancer cells and in HCC4006 human lung cancer cells. than in COS-7 and CHO cells and in a ligand-dependent manner.
- Ep 98 with Dr. GPCR Team
As a young researcher fascinated by chemokine receptors, molecular pharmacology, drug discovery, and immuno-oncology GPCR Ecosystem About Monserrat Avila Zozaya I am a cell biologist interested in studying GPCRs, especially developed expertise over the past two decades studying structure/function relationships of GPCRs using live-cell Her work focused on chemokine receptors, members of the GPCR family that control cell movement in the
- The combination of brentuximab vedotin and chidamide synergistically suppresses the proliferation of T-cell lymphoma cells through the enhancement of apoptosis
combination of brentuximab vedotin and chidamide synergistically suppresses the proliferation of T-cell lymphoma cells through the enhancement of apoptosis Published date November 3, 2023 Abstract " Purpose : Peripheral T-cell lymphoma (PTCL) is an aggressive disease with a poor prognosis. HH cells, originating from an aggressive cutaneous T-cell lymphoma, were used as an experimental model The combined effects of chidamide and BV were demonstrated in a study of HH-cell xenograft mice; mean
- Blockade of vasoactive intestinal peptide receptor 2 (VIPR2) signaling suppresses cyclin D1-dependent cell-cycle progression in MCF-7 cells
progression in MCF-7 cells Published date March 1, 2024 Abstract "Vasoactive intestinal peptide (VIP In this study, we examined the role of VIPR2 in cell cycle progression. in MCF-7 cells. The percentage of cells in the S-M phase was decreased in MCF-7 cells treated with KS-133. In MCF-7 cells stably-expressing VIPR2, KS-133 decreased PI3K activity and cAMP levels.
- Autocrine proteinase-activated receptor signaling in PC3 prostate cancer cells
in Oncology and Immunology Autocrine proteinase-activated receptor signaling in PC3 prostate cancer cells PARs are highly expressed in many cancer cells, including prostate cancer (PCa), and regulate various In this study, we examined the androgen-independent human prostatic cancer cell line PC3 and find the Using genetically encoded PAR cleavage biosensors, we showed that PC3 cells secrete proteolytic enzymes of PAR1 promotes PC3 cell migration and suppresses cell proliferation, whereas PAR2 deficiency showed
- Vasoactive intestinal peptide receptor 2 signaling promotes breast cancer cell proliferation by enhancing the ERK pathway
in Oncology and Immunology Vasoactive intestinal peptide receptor 2 signaling promotes breast cancer cell cell lines, promoted cell proliferation. proliferation in VIPR2-overexpressing MCF-7 and MDA-MB-231 cells. was greater than that in control cells, suggesting the increased PKA activity. at the same level as observed in EGFP-expressing cells treated with U0126.
- NPFF stimulates human ovarian cancer cell invasion by upregulating MMP-9 via ERK1/2 signaling
< GPCR News < GPCRs in Oncology and Immunology NPFF stimulates human ovarian cancer cell invasion by The TaqMan probe-based RT-qPCR showed that NPFF and NPFFR2 were expressed in three human EOC cells, CaOV3 In comparison, NPFF and NPFFR2 expression levels were higher in SKOV3 cells than in CaOV3 or OVCAR3 cells Treatment of SKOV3 cells with NPFF did not affect cell viability and proliferation but stimulated cell This study provides evidence that NPFF stimulates EOC cell invasion by upregulating MMP-9 expression
- Respiratory infections predominate after day 100 following B-cell maturation antigen-directed CAR T-cell therapy
News < GPCRs in Oncology and Immunology Respiratory infections predominate after day 100 following B-cell maturation antigen-directed CAR T-cell therapy Published date September 26, 2023 Abstract "Infections are an important complication after B-cell maturation antigen (BCMA)-directed chimeric antigen receptor (CAR) T-cell therapy and risks may differ between the early and late periods. Respiratory infections predominate after BCMA CAR T-cell therapy, particularly after day 100.
- Simultaneous activation of CXC chemokine receptor 4 and histamine receptor H1 enhances calcium signaling and cancer cell migration
activation of CXC chemokine receptor 4 and histamine receptor H1 enhances calcium signaling and cancer cell Here, we show that HRH1 is widely expressed in various cancer cell lines and cancer tissues and that that endogenously express CXCR4 and HRH1 but not in cells deficient in CXCR4 or HRH1. while histamine alone does not induce cell migration. Enhanced calcium signaling and cell migration are also observed in NCI-H23 and HeLa cells, which coexpress
- The EBI2 receptor is coexpressed with CCR5 in CD4+ T cells and boosts HIV-1 R5 replication
< GPCR News < GPCRs in Oncology and Immunology The EBI2 receptor is coexpressed with CCR5 in CD4+ T cells Methods: We identified GPCRs expressed in primary CD4+CCR5+ T cells by multi-RT-qPCR. Cell lines expressing EBI2 were established by transduction with HIV vectors. The amount of HIV reverse transcripts was similar in cells expressing or not EBI2. Conclusions: EBI2 expression in CD4+CCR5+ cells boosts HIV-1 R5 productive infection.
- Activation of orphan receptor GPR132 induces cell differentiation in acute myeloid leukemia
< GPCR News < GPCRs in Oncology and Immunology Activation of orphan receptor GPR132 induces cell differentiation Here, we showed that genetic activation of the orphan GPCR GPR132 significantly induced cell differentiation Notably, GPR132 activation by 8GL promoted differentiation and reduced colony formation in human AML cell We further showed that the combination of 8GL and an mTOR inhibitor synergistically elicited AML cell impaired tumor growth and extended mouse survival in an AML xenograft model accompanied by enhanced cell
- Stimulation of ectopically expressed muscarinic receptors induces IFN-γ but suppresses IL-2 production by inhibiting activation of pAKT pathways in primary T cells
induces IFN-γ but suppresses IL-2 production by inhibiting activation of pAKT pathways in primary T cells signaling molecules and the phosphatidylinositol, Ras, MAPK, and PI3 kinase pathways, leading to T cell This may explain the inhibitory impact on IL-2 production in hM3Dq/β1T cells. that activating the pAKT pathway is critical for IL-2 production in T cells." Tags GPCR; T cells; muscarinic receptor; signaling.
- miR-19a may function as a biomarker of oral squamous cell carcinoma (OSCC) by regulating the signaling pathway of miR-19a/GRK6/GPCRs/PKC in a Chinese population
< GPCR News < GPCRs in Oncology and Immunology miR-19a may function as a biomarker of oral squamous cell Using a transcriptomic analysis of over 37,000 nuclei, we identified twelve distinct clusters of cells corresponding to temperature-sensing arista neurons, humidity-sensing sacculus neurons, and support cells We found that each cell type could be characterized by a unique expression profile of ion channels, GPCR signaling molecules, synaptic vesicle cycle proteins, and cell adhesion molecules.
- Molecular characterization of breast cancer cell pools with normal or reduced ability to respond to progesterone: a study based on RNA-seq
< GPCR News < GPCRs in Oncology and Immunology Molecular characterization of breast cancer cell pools Methods: We disrupted the PR gene (PGR) in ERα-positive/PR-positive T-47D cells using the CRISPR/Cas9 to control T-47D cells. We analyzed the gene expression profiles of PR-low and control T-47D cells in the absence of hormone More than 6000 genes were DE in control T-47D cells upon stimulation with P4 or P4 plus E2.
- Opposite Effects of Src Family Kinases on YAP and ERK Activation in Pancreatic Cancer Cells: Implications for Targeted Therapy
and Immunology Opposite Effects of Src Family Kinases on YAP and ERK Activation in Pancreatic Cancer Cells IGF-1 receptors and G protein-coupled (GPCR) signaling systems leading to mitogenic signaling in PDAC cells agonist neurotensin induced rapid activation of Src family of tyrosine kinases (SFK) within PANC-1 cells by FAK phosphorylation at Tyr576/577 and Tyr861, sensitive biomarkers of SFK activity within intact cells and suppressed the growth of Mia PaCa-2 cells xenografted into the flank of nude mice.
- Systems Pharmacodynamic Model of Combination Gemcitabine and Trabectedin in Pancreatic Cancer Cells. Part II: Cell Cycle, DNA Damage Response, and Apoptosis Pathways
Immunology Systems Pharmacodynamic Model of Combination Gemcitabine and Trabectedin in Pancreatic Cancer Cells Part II: Cell Cycle, DNA Damage Response, and Apoptosis Pathways Published date October 31, 2023 Abstract , we reported the synergistic cytotoxic effects of gemcitabine and trabectedin on pancreatic cancer cells Here we describe drug effects on pathways associated with cell cycle, DNA damage response (DDR), and The SPD model was subsequently incorporated into our previously-established cell cycle model, forming
- Activation of PI3K/Akt pathway by G protein-coupled receptor 37 promotes resistance to cisplatin-induced apoptosis in non-small cell lung cancer
pathway by G protein-coupled receptor 37 promotes resistance to cisplatin-induced apoptosis in non-small cell protein-coupled receptor 37 (GPR37) exhibits unknown functions in tumors, particularly in non-small-cell We detected the expression of GPR37 in NSCLC tissues and cell lines. The study explored the influence of GPR37 on tumor cell proliferation. Furthermore, we examined the effects of GPR37 on tumor cell apoptosis and invasion.
- Metallo-protease Peptidase M84 from Bacillusaltitudinis induces ROS-dependent apoptosis in ovarian cancer cells by targeting PAR-1
Metallo-protease Peptidase M84 from Bacillusaltitudinis induces ROS-dependent apoptosis in ovarian cancer cells This metallo-protease had no discernible impact on normal cell survival, but it specifically induced apoptosis in ovarian cancer cells. PAR-1, a GPCR which is reported to be overexpressed in ovarian cancer cells, was identified as a target This evoked apoptotic death of the ovarian cancer cells through the intrinsic route.
- LPA1-mediated inhibition of CXCR4 attenuates CXCL12-induced signaling and cell migration
in Oncology and Immunology LPA1-mediated inhibition of CXCR4 attenuates CXCL12-induced signaling and cell Results: We observed that CXCR4 forms heteromers with LPA1 in recombinant HEK293A cells and the human breast cancer cell line MDA-MB-231. MDA-MB-231 cells but not in LPA1-deficient cells. have been evidenced across various cell lines.
- RGS20 promotes non-small cell lung carcinoma proliferation via autophagy activation and inhibition of the PKA-Hippo signaling pathway
< GPCR News < GPCRs in Oncology and Immunology RGS20 promotes non-small cell lung carcinoma proliferation CCK8 and cell cloning were conducted to determine the proliferation ability of H1299 and Anip973 cells Further, RGS20 accelerated cell proliferation by increasing autophagy. in RGS20 knock-down cells. Conclusion: Our findings indicate that RGS20 drives NSCLC cell proliferation by triggering autophagy
- GPR4 in the pH-dependent migration of melanoma cells in the tumor microenvironment
< GPCR News < GPCRs in Oncology and Immunology GPR4 in the pH-dependent migration of melanoma cells in pH-gradient) is a well-known driver of tumor progression and metastasis. In this study, we investigated the pH-dependent migration of GPR4 wildtype/overexpressing SK-Mel-28 cells Migration of GPR4 overexpressing SK-Mel-28 cells was enhanced in a range of pH 6.5 - pH 7.5 as compared Results indicate that GPR4 is involved in the migration of melanoma cells, especially in the tumor periphery
- Single cell G-protein coupled receptor profiling of Transcription factor 21 expressing activated kidney fibroblasts
< GPCR News < GPCRs in Oncology and Immunology Single cell G-protein coupled receptor profiling of Transcription Key results: Transcription factor 21 (Tcf21)+ cells that expressed 2 or 3 of Postn, Acta2 and Pdgfra Tcf21+ α-smooth muscle actin (α-SMA)+ interstitial cells accumulated in the kidneys of mice with UUO TCF21, ADGRA2, S1PR3 and ADORA2A/2B were each detectable in α-SMA+ interstitial cells in human kidneys Study of GPCRs expressed by these cells may identify new opportunities for CKD therapeutics. " Authors
- High expression of GPR50 promotes the proliferation, migration and autophagy of hepatocellular carcinoma cells in vitro
expression of GPR50 promotes the proliferation, migration and autophagy of hepatocellular carcinoma cells was analyzed in HCC patients (gene expression omnibus database (GEO) (GSE45436)) and detected in HCC cell 7919, and the results showed that GPR50 was significantly up-regulated in HCC patients and CBRH-7919 cell Gpr50 cDNA was transfected into HCC cell line CBRH-7919, and we found that Gpr50 promoted the proliferation Cuifang Chang Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call
- Dynamic Phosphoproteomics of BRS3 Activation Reveals the Hippo Signaling Pathway for Cell Migration
and Immunology Dynamic Phosphoproteomics of BRS3 Activation Reveals the Hippo Signaling Pathway for Cell The lung cancer cell line H1299-BRS3 was treated with MK-5046, an agonist of BRS3, for different durations Harvested cellular proteins were digested and phosphopeptides were enriched by immobilized titanium ( dephosphorylation and nucleus localization of the Yes-associated protein (YAP), and its association with cell Our data collectively demonstrate that BRS3 activation contributes to cell migration through downregulation