Oncology and Immunology Comparative analysis of the occupancy of Histone H3 Lysine 4 methylation in the cells 28, 2023 Abstract "In this current study, we have compared our H3K4me3 Chip-Sequencing data in PC3 cells in response to 6h and 24h TGFβ stimulation with the IFNγ stimulated/unstimulated HeLa S3 cells Since modification changes in response to TGFβ and IFNγ and compare them to explore the genes common to both as well Noopur Thakur Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

She has expertise in cell biology, pharmacology, translational medicine, oncology, immuno-oncology, immunology Previously, she held the position of Vice President of Cell Biology & Pharmacology, at Bristol-Myers She has extensive experience working with Biologics, and Antibody Drug Conjugates as well as experience

kinase (MAGUK) scaffold protein, and protein kinase A anchoring protein (AKAP) 5 in MCF7 breast cancer cells MCF7 breast cancer cells express GPR30, β1AR, MAGUKs, and AKAP5 in the plasma membrane, and co-immunoprecipitation Furthermore, expression of GPR30 in MCF7 cells constitutively and PDZ-dependently inhibits β1AR-mediated These results argue that GPR30 and β1AR form a PDZ-independent complex in MCF7 cells through which GPR30 adrenergic receptor Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

G protein signaling protein 6 alleviates acute lung injury by inhibiting inflammation and promoting cell Organoid culture was used to assess the stemness and self-renewal capacity of alveolar epithelial type II cells in macrophages and decreased apoptosis in epithelial cells. a protective role in ALI not only in early inflammatory responses but also in endogenous lung stem cell Zhou , Linlin Wang , Yu Yan , Jun She , Lin Tong , Yuanlin Song Tags Acute lung injury , Apoptosis , Cell-renewal

< GPCR News < GPCRs in Oncology and Immunology A GPCR checkpoint drives CD8+ T cell dysfunction and immunotherapy Authors listed Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

Immunology [1,2,4]Triazolo[1,5-c]pyrimidines as Tools to Investigate A3 Adenosine Receptors in Cancer Cell Compound 20 has been tested on both A3 adenosine receptor positive cancer cell lines (CHO-A3AR transfected , THP1 and HCCT26) and in A3 negative cancer cell lines, showing no effect on the latter and a proliferative antagonists Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

Oncology and Immunology Enterococcus-derived tyramine hijacks α2A-adrenergic receptor in intestinal stem cells disease (IBD) is characterized by dysbiosis of the gut microbiota and dysfunction of intestinal stem cells Using an engineered tyrDC-deficient Enterococcus faecalis strain and intestinal epithelial cell-specific adrenergic receptor Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

Oncology and Immunology Gi/o GPCRs drive the formation of actin-rich tunneling nanotubes in cancer cells Using a model of human adrenocortical cancer cells, here we established that activation of the GPCR OXER1 acid (5-oxo-ETE), leads to the formation of filopodia-like elongated projections connecting adjacent cells Kazanietz Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

of the nematode-trapping fungus Arthrobotrys flagrans activates mitochondria and reprograms fungal cells GPCR, GprC, at the plasma membrane and together with the G-protein alpha subunit GasA, reprograms the cell Reinhard Fischer Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

Methods: GPR81 was stably knocked down (KD) in MCF-7 human breast cancer cells which were subjected to RNA-seq analysis, 3D growth, in situ- and immunofluorescence analyses, and cell viability- and motility Key findings were additionally studied in other breast cancer cell lines and in mammary epithelial cells Single cell in situ analysis of MCF-7 cells revealed that several GPR81-regulated genes were upregulated in the same cell clusters.

Low extracellular pH confers radioresistant tumors to glial cells. inhibitor of GPR68 named Ogremorphin (OGM) to induce the iron mediated cell death pathway: ferroptosis Next, A GPI-anchored pH reporter, pHluorin2, was stably expressed in U87 glioblastoma cells to probe Cell survival assays, via nuclei counting and cell titer glo, were used to demonstrate sensitivity to To determine GPR68 inhibition's mechanism of cell death we use DAVID pathway analysis of RNAseq.

developed expertise over the past two decades studying structure/function relationships of GPCRs using live-cell Her work focused on chemokine receptors, members of the GPCR family that control cell movement in the As a young researcher fascinated by chemokine receptors, molecular pharmacology, drug discovery, and immuno-oncology Monserrat Avila Zozaya I did a PhD in cell biology at CINVESTAV, Mexico. My long-term interest is focused on understanding the mechanisms mediated by GPCRs at the cellular communication

and hypoxia on migration, proliferation, invasion, and signaling in 2D and 3D models of breast cancer cell Antagonizing NPY1R and/or NPY5R in hypoxia compared to normoxia more greatly reduced MAPK signaling, cell proliferation, cell migration and invasion, and spheroid growth and invasion. The estrogen receptor positive MCF7 cells were significantly less invasive in 3D spheres when NPY5R was There were some discrepancies in the responses of each cell line to the isoform-specific antagonists

Experimental design: We performed targeted sequencing, high-throughput drug screening, and single-cell genomic profiling on leukemia cell samples derived from patients with AML. understand the co-occurring mutation patterns and further investigated the mutation profiles in the single cells The application of single-cell genomic sequencing unveiled the co-occurrence of variants at the individual cell level, highlighting the presence of distinct subclones within patients with AML.

previously showed that ColI(2.3)+/Rs1+ mice, in which Gs-GPCR signaling was hyper-activated in osteoblastic cell signaling pathway has been implicated in the pathogenesis of FD-like bone, but the specific Wnts and which cells Single-cell RNA sequencing on long-bone stromal cells of 9-wk-old male ColI(2.3)+/Rs1+ mice and littermate controls showed that fibroblastic stromal cells in ColI(2.3)+/Rs1+ mice were expanded. cells.

Increasing evidence reveals the heightened expression of olfactory receptors in tumorous tissues and cells Olfactory receptors have demonstrated influence over tumor cell proliferation and metastasis, establishing Guo-Tai Wang Tags G Protein-coupled receptors , Olfactory receptors , Therapeutic targeting , Tumor cell Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call for GPCR

Immunology Expression pattern and clinical significance of beta 2-adrenergic receptor in oral squamous cell However, the role of β2-AR in oral cancer is not well identified. significance in relation with the clinicopathological features and overall survival of oral squamous cell Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call for GPCR

Depletion of GPR37 significantly reduced CRC tumor cell growth both in vitro and in vivo. Further tests showed that GPR37 protects cancer cells from ferroptosis by upregulating SCD1 expression Mechanistic studies have shown that GPR37 modulates lipid metabolism in tumor cells by promoting SCD1 SCD1 , p38 Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

receptors, are the targets of multiple drugs displaying anti-growth effects in large-scale, cancer cell , Guanming Wu, Gioacchino Natoli, J Silvio Gutkind , Francesco Raimondi Tags GPCR , cancer , cancer cell lines , cell-cell communication , drug repurposing , personalized medicine , signaling network , survival Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call for GPCR

Oncology and Immunology GPR176 Promotes Cancer Progression by Interacting with G Protein GNAS to Restrain Cell Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call for GPCR

protein-coupled receptors (GPCRs), enhancing proliferation, adhesion, and migration in many types of cancer cells Our group previously reported that LPA induces production of CCN1 protein in human prostate cancer cell In these cells, the mitogenic activity of LPA is mediated by LPA Receptor 1 (LPAR1), a GPCR. of CCN proteins by LPA, and by the related lipid mediator sphingosine-1-phosphate (S1P), in various cellular In some model systems, CCN1 and CCN2 play key roles in LPA/S1P-induced cell migration and proliferation

Mesenchymal cells play key roles in numerous developmental processes. subunits Gαq and Gα11 (Gαq/11) transmit mechanical and chemical signals to activate TGFβ in epithelial cells exhibited abnormal alveolar development, with suppressed myofibroblast differentiation, altered mesenchymal cell synthetic function, and reduced lung TGFβ2 deposition, as well as kidney abnormalities. undescribed mechanism of cyclical stretch-induced Gαq/11-dependent TGFβ2 signalling in mesenchymal cells

Its high expression on the surface of multiple myeloma cells has rendered it an attractive target for therapeutic interventions, including monoclonal antibodies, CAR-T cells, and T-cell engagers. Liu, Fei Xu Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

post-translational modification that supports membrane interactions of proteins involved in various cellular We examined the prenylation of carboxy-terminus (Ct) mutated Gγ in cells exposed to Fluvastatin and prenyl monitored the subcellular localization of fluorescently tagged Gγ subunits and their mutants using live-cell Given the cell and tissue-specific expression of different Gγ subtypes, our findings indicate a plausible mechanism allowing for statins to differentially perturb heterotrimeric G protein signaling in cells

Individual cells trend to adapt environmental changes in a "whole" flora through communications, allowing Furthermore, GprC and AcyA regulate the primary metabolism as well as secondary metabolism, and further These studies highlight a crucial G protein signaling pathway for cell communication that is mediated ochraceus, hoping to achieve comprehensive prevention and control of mycotoxin pollution from interrupting cell Authors Jing Gao, Huiqing Liu, Yuxin Jin, Yunbo Luo, Kunlun Huang, Zhihong Liang Tags Cell communication

heterotrimeric G-proteins (Gαβγ) by G-protein-coupled receptors (GPCRs) is a quintessential mechanism of cell disrupted their engagement with GIV/Girdin, thereby blocking noncanonical G-protein signaling in tumor cells and inhibiting proinvasive traits of metastatic cancer cells. Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call for GPCR

Here, we showed that US28 increased the malignancy of U251 glioblastoma cells by enhancing signaling Enhanced S1P signaling promoted glioblastoma cell proliferation and survival by activating the kinases US28 also activated the S1P signaling axis in HCMV-infected cells. Authors Nick D Bergkamp , Jeffrey R van Senten , Hendrik J Brink , Maarten P Bebelman , Jelle van den Martine J Smit Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

MM) is a complex hematological malignancy characterized by abnormal antibody production from plasma cells G-protein-coupled receptor class C group 5 member D (GPRC5D), an orphan GPCR predominantly expressed in MM cells Talquetamab, a Food and Drug Administration-approved T-cell-directing bispecific antibody developed for talquetamab Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call