Search Results
276 items found for "Cell cycle"
- Structures of oxysterol sensor EBI2/GPR183, a key regulator of the immune response
August 2022 "Oxysterols induce the migration of B-lymphocytes and dendritic cells to interfollicular oxysterol binding site and the Gαi interface attenuate G protein signaling and abolish oxysterol-mediated cell
- Navigating the Signaling Network: RTK and GPCR Crosstalk Uncovered
On the other hand, RTKs play pivotal roles in growth factor signaling, regulating cell growth, differentiation phospho-mimicking mutants and assays such as linear ion trap mass spectrometry, BRET, pull down, and transwell cell growth factor signaling might contribute to altered cellular behaviors in cancer, promoting uncontrolled cell
- 📰 GPCR Weekly News, August 14 to 20, 2023
Vladimir Katanaev and colleagues identified compromised GPCR signaling in cancer cells using improved Improved approaches to channel capacity estimation discover compromised GPCR signaling in diverse cancer cells S1P1 signaling axis Methods & Updates in GPCR Research Magnetic Liposomes Infused with GPCR-Expressing Cell Sewage Optogenetic manipulation of Gq- and Gi/o-coupled receptor signaling in neurons and heart muscle cells
- Endosomal parathyroid hormone receptor signaling
assumes that stimulation of heterotrimeric G protein signaling upon ligand binding occurs solely at the cell Internalized receptors can then recycle back to the cell surface or be trafficked to lysosomes for degradation
- 📰 GPCR Weekly News, June 5 to 11, 2023
Autocrine Proteinase-Activated Receptor (PAR) signaling in PC3 prostate cancer cells. & Updates in GPCR Research A Peptidisc-based Survey of the Plasma Membrane Proteome of a Mammalian Cell GPCRs in Immunology and Oncology The GPCR–Gαs–PKA signaling axis promotes T cell dysfunction and cancer and Webinars GPCRs in drug discovery: challenges & solutions (June 19 - 23, 2023) Training School on “Cell-based
- The regulation of PKA signaling in obesity and in the maintenance of metabolic health
October 2022 "The cAMP-dependent protein kinase (PKA) system represents a primary cell-signaling pathway Neural PKA signaling is regulated by afferent and peripheral efferent signals that link specific neural cell
- 📰 GPCR Weekly News, February 27 to March 5, 2023
Endocrinology, and Taste Dietary compounds activate an insect gustatory receptor on enteroendocrine cells Establishment of a CaCC-based Cell Model and Method for High-throughput Screening of M3 Receptor Drugs Structural details of a Class B GPCR-arrestin complex revealed by genetically encoded crosslinkers in living cells Heptares and Neurocrine Biosciences won Top Out-Licensing Deal on the 8th annual Japan Deal of the Year Call
- β-arrestin1 and 2 exhibit distinct phosphorylation-dependent conformations when coupling to the...
2 exhibit distinct phosphorylation-dependent conformations when coupling to the same GPCR in living cells Employing GRK knockout cells and β-arrestins lacking the finger-loop-region, we show that the two isoforms
- The Adhesion GPCR VLGR1/ADGRV1 Regulates the Ca2+ Homeostasis at Mitochondria-Associated ER Membrane
associated with the human Usher syndrome (USH), the most common form of inherited deaf-blindness as well interactome of VLGR1, molecules are enriched that are associated with both the ER and mitochondria, as well proteins of MAMs by pull-down assays in vitro complemented by in situ proximity ligation assays in cells The absence of VLGR1 in tissues and cells derived from VLGR1-deficient mouse models resulted in alterations
- N-Acyl Amides from Neisseria meningitidis and Their Role in Sphingosine Receptor Signaling
Both groups of metabolites suppress anti-inflammatory interleukin-10 signaling in human macrophage cell they also suppress the pro-inflammatory interleukin-17A+ population in TH 17-differentiated CD4+ T cells
- Targeted Activation of G-Protein Coupled Receptor-Mediated Ca 2+ Signaling Drives Enhanced Cartilage
processes that govern chondrocyte gene expression, protein synthesis, cytoskeletal remodeling, and cell synthetically initiate robust [Ca2+]i transients and oscillatory [Ca2+]i signaling in chondrocyte-like ATDC5 cells under repeated CNO stimulation modified the temporal dynamics of hM3Dq [Ca2+]i signaling, increased cell enhanced matrix production in a CNO dose- and frequency-dependent manner, and triggered the formation of cell
- A broad look into the future of systemic sclerosis
Fibroblasts are a key effector cell in SSc. Promising nuclear receptors as key regulators of transcriptional programmes will be introduced as well Fibroblasts from SSc patients exhibit a specific signalling and reactivate developmental pathways and stem cell
- Microbial Metabolites Orchestrate a Distinct Multi-Tiered Regulatory Network in the Intestinal Epith
RNA sequencing of intestinal epithelial cells responding to butyrate and secondary bile acids in combination Using shRNA knockdown and CRISPR/Cas9 knockout cell lines, as well as mouse models, we confirmed the
- 📰 GPCR Weekly News, June 12 to 18, 2023
GPCRs in Oncology and Immunology The GPCR-Gαs-PKA signaling axis promotes T cell dysfunction and cancer induces IFN-γ but suppresses IL-2 production by inhibiting activation of pAKT pathways in primary T cells zoominar (June 22, 2023) NEW Meet Inoviem Scientific Team (June 19 - 23, 2023) Training School on “Cell-based
- VAMP2: a crucial player in the delivery of MOR to the synapse
complex, including vesicle-associated membrane protein 2 (VAMP2), regulate this last step toward the cell characterized, the mechanisms that modulate the delivery of GPCRs to the membrane cell still need to be well known [3-5]. in regulating dopamine D2 receptor signaling by controlling the trafficking of the receptor to the cell presence of VAMP2, specifically in recycling vesicles containing MOR but not B2AR or TFR in HEK293 cells
- 📰 GPCR Weekly News, June 3 to June 9, 2024
uncover GPR183 as a key mediator for methionine to stimulate milk synthesis in mouse mammary epithelial cells domain-based fluorescent reporters for visualizing phosphatidylinositol 4-phosphate dynamics in living cells Scientist Senior Research Associate/Associate Scientist, Protein Science Post-Doctoral Fellow—Pharmacology/Cell
- Cholesterol-Dependent Dynamics of the Serotonin1A Receptor Utilizing Single Particle Tracking: ...
Particle Tracking: Analysis of Diffusion Modes "G protein-coupled receptors (GPCRs) are signaling hubs in cell Notably, in cholesterol-depleted cells, we observed an increase in the confined population of the receptor
- Cholesterol-Dependent Dynamics of the Serotonin 1A Receptor Utilizing Single Particle Tracking....
September 2022 "G protein-coupled receptors (GPCRs) are signaling hubs in cell membranes that regulate Notably, in cholesterol-depleted cells, we observed an increase in the confined population of the receptor
- Transformative GPCR Insights: Unleash New Horizons in Science | Sep 9 - 15, 2024
Crinetics Pharmaceuticals Announces September 2024 Inducement Grants Under Nasdaq Listing Rule 5635(c)(4) Call , 2024 | 4th Transatlantic ECI GPCR Symposium September 18, 2024 | FREE Webinar - The value of GPCR cell-based Molecular Pharmacology - The Hauser Group NEW Postdoctoral Scholar – iPSC in cardiac and endothelial cell Class A GPCR-biased ligands GPCRs in Cardiology, Endocrinology, and Taste CRTC1 in Mc4r-expressing cells
- GPR84 signaling promotes intestinal mucosal inflammation via enhancing NLRP3 inflammasome activation
medium-chain free fatty acid receptor GPR84 is a G protein-coupled receptor primarily expressed in myeloid cells These results define a unique role of GPR84 in innate immune cells and intestinal inflammation, and suggest
- Unlocking the Therapeutic Potential of Previously Undruggable GPCRs
of several hundred candidate hits, enriched because they either bind with increased affinity at the cell Figure 4: Payload delivery using Orion’s superagonist analogs on target cells expressing CCR5. at killing cells than unconjugated MMAE, the Orion CCL5 superagonist analog significantly increased both the potency and selectivity of the payload towards target cells. Figure 5: In an in vitro functional signaling assay on a human monocytic cell line, OB-004 demonstrated
- Pharmacophore-guided Virtual Screening to Identify New β 3 -adrenergic Receptor Agonists
Out of 35 tested compounds, 4 compounds were active in CHO-K1 cells expressing the human β3 -AR, and 8 compounds were active in CHO-K1 cells expressing the mouse β3 -AR."
- Gαs and Gαq/11 protein coupling bias of two AVPR2 mutants (R68W and V162A) that cause nephrogenic di
protein coupled receptor (GPCR) and mainly couples with Gαs protein leading to cAMP accumulation in the cell showed the ability to couple with Gαq/11 protein and make Ca2+ mobilization at different levels in the cell
- Dr. GPCR University registration is now open! Secure your spot now!
Genomic Activity of an EP4-Receptor and β2-Adrenoceptor Agonist in BEAS-2B Human Bronchial Epithelial Cells Mechanistic insights from the atomic-level quaternary structure of short-lived GPCR oligomers in live cells November 5 - 7, 2024 | 16th Annual PEGS Europe September 18, 2024 | FREE Webinar - The value of GPCR cell-based
- The mouse cytomegalovirus G protein-coupled receptor homolog, M33, coordinates key features of ...
The mouse cytomegalovirus (MCMV) GPCR, M33, facilitates dendritic cell (DC)-dependent viremia, the extravasation M33 engages Gq/11 to constitutively activate phospholipase C β (PLCβ) and downstream cyclic AMP response-element IMPORTANCE G protein-coupled receptors (GPCRs) act as cell surface molecular "switches" which regulate The mouse CMV (MCMV) GPCR homolog, designated M33, is important for cell-associated virus spread and
- GPR15 expressed in T lymphocytes from RA patients is involved in leukocyte chemotaxis to the...
the synovium "The rheumatoid arthritis (RA) inflammatory process occurs in the joints where immune cells evaluated the expression of GPR15 and GPR15L in blood and synovial tissue samples from RA patients, as well synovial fluid and peripheral blood were analyzed for CD45+CD3+CD4+GPR15+ and CD45+CD3+CD8+GPR15+ T cell
- APEX2/AUR Biosensor: A Powerful Tool for Protein Interaction and Trafficking
Therefore, it’s a great molecular tool for proximity labeling approaches in living cells. APEX2 biosensor utilizes a fluorogenic substrate called Amplex UltraRed (AUR).
- Regulation of rod photoreceptor function by farnesylated G-protein γ-subunits
G-protein transducin, Gt, is a key signal transducer and amplifier in retinal rod and cone photoreceptor cells Immunohistochemistry and Western blotting demonstrated the robust expression of each transgenic Gγ in rod cells