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308 items found for "T cells"

  • Navigating the Signaling Network: RTK and GPCR Crosstalk Uncovered

    One fascinating aspect of the cellular signaling network is the crosstalk between G protein-coupled receptors On the other hand, RTKs play pivotal roles in growth factor signaling, regulating cell growth, differentiation phospho-mimicking mutants and assays such as linear ion trap mass spectrometry, BRET, pull down, and transwell cell These findings have broad implications for understanding cellular signaling in both normal physiology behaviors in cancer, promoting uncontrolled cell migration and invasion.

  • 📰 GPCR Weekly News, March 25 to March 31, 2024

    Smith for their research on β2-adrenergic receptor associates with CXCR4 multimers in human cancer cells phosphorylation After wounding, a G-protein coupled receptor promotes the restoration of tension in epithelial cells of dynamic cAMP responses to GPCR agonists for exploration of GLP-1R ligand effects in pancreatic β-cells Oncology and Immunology The β2-adrenergic receptor associates with CXCR4 multimers in human cancer cells Announces Publication in PNAS Using Cutting Edge Spectroscopy to Detect GPCR Heteromers on Live Cancer Cells

  • GPCR/endocytosis/ERK signaling/S2R is involved in the regulation of the internalization...

    activating properties of human salivary histatin 1 "Human salivary histatin 1 (Hst1) exhibits a series of cell-activating properties, such as promoting cell spreading, migration, and metabolic activity. We also adopted live cell imaging to monitor the whole intracellular trafficking process of F-Hst1. Our results showed that the inhibition of cellular respiration hindered the internalization of F-Hst1 reduced the uptake, which was accompanied by the nullification of the promoting effect of F-Hst1 on cell

  • 📰 GPCR Weekly News, February 26 to March 3, 2024

    Robert Lefkowitz for their work on GPCRs: from radioligand binding to cellular signaling Dr. neurological and psychiatric disorders: An in silico approach Opioid modulation of prefrontal cortex cells and circuits GPCRs in Oncology and Immunology RGS20 promotes non-small cell lung carcinoma proliferation Blockade of vasoactive intestinal peptide receptor 2 (VIPR2) signaling suppresses cyclin D1-dependent cell-cycle progression in MCF-7 cells Methods & Updates in GPCR Research Cryo-electron microscopy for GPCR research

  • CCL25/CCR9 interaction promotes the malignant behavior of salivary adenoid cystic carcinoma via...

    In vitro, SACC cell proliferation and apoptosis were evaluated using Cell Counting Kit-8 and colon formation , and cell migration and invasion were detected by wound healing and transwell assays.

  • Statin-induced increase in actin polymerization modulates GPCR dynamics and compartmentalization

    September 2022 "The function of the actin cytoskeleton in cellular motility and trafficking has been For this, we analyzed the lateral dynamics of the serotonin1A receptor in cholesterol-depleted cells Our results indicate that lateral diffusion parameters of serotonin1A receptors in normal cells are consistent Interestingly, these parameters are altered in cholesterol-depleted cells and the receptor exhibits dynamic

  • What's Going On with GPCRs?! Find Out in This Week's Update! ⦿ Nov 4 - 10, 2024

    As a premium member , you have access to all the complimentary perks as well as: Dr. CB2 Targeting Drug Development Summit November 25 - 26, 2024 | 6th International Conference on Stem Cell 2025 July 12 - 17, 2026 | 20th World Congress of Basic and Clinical Pharmacology GPCR Jobs Scientist I Cell in Molecular Pharmacology - The Hauser Group Postdoctoral Scholar – iPSC in cardiac and endothelial cell

  • 📰 GPCR Weekly News, August 14 to 20, 2023

    Vladimir Katanaev and colleagues identified compromised GPCR signaling in cancer cells using improved Improved approaches to channel capacity estimation discover compromised GPCR signaling in diverse cancer cells S1P1 signaling axis Methods & Updates in GPCR Research Magnetic Liposomes Infused with GPCR-Expressing Cell Sewage Optogenetic manipulation of Gq- and Gi/o-coupled receptor signaling in neurons and heart muscle cells

  • GPCRs Are Optimal Regulators of Complex Biological Systems and Orchestrate the Interface between ...

    GPCRs have been long considered as controllers of communication between tissues and cells. This communication involves the ligand-mediated control of cell surface receptors that then direct their stimuli to impact cell physiology. considerable focus has been placed on the ability of these therapeutics to modulate diseases by acting at cell The ability of these receptorsomes (often in the absence of typical cell surface ligands) to control

  • Fentanyl activates ovarian cancer and alleviates chemotherapy-induced toxicity via opioid...

    the direct role of fentanyl on cancer, little is known on the effect of fentanyl on ovarian cancer cells Methods Proliferation, migration and apoptosis assays were performed in ovarian cancer cells after fentanyl

  • N-terminal alterations turn the gut hormone GLP-2 into an antagonist with gradual loss of GLP-2 ...

    Experimental approach: COS-7 cells were transfected with the human GLP-2R and assessed for cAMP accumulation To examine selectivity, COS-7 cells expressing human GLP-1 or GIP receptors were assessed for cAMP accumulation

  • Exciting GPCR Events for Next Year! + GPCR Weekly Rocket Launch ⦿ Oct 28 - Nov 3, 2024

    Therapeutics, Inc. has received Orphan Drug Designation (ODD) from the FDA for its hematopoietic stem cell 2024 | 16th Annual PEGS Europe   NEW November 25 - 26, 2024 | 6th International Conference on Stem Cell 2025 July 12 - 17, 2026 | 20th World Congress of Basic and Clinical Pharmacology GPCR Jobs Scientist I Cell in Molecular Pharmacology - The Hauser Group Postdoctoral Scholar – iPSC in cardiac and endothelial cell

  • 📰 GPCR Weekly News, June 3 to June 9, 2024

    uncover GPR183 as a key mediator for methionine to stimulate milk synthesis in mouse mammary epithelial cells domain-based fluorescent reporters for visualizing phosphatidylinositol 4-phosphate dynamics in living cells Scientist Senior Research Associate/Associate Scientist, Protein Science Post-Doctoral Fellow—Pharmacology/Cell

  • Actions of Parathyroid Hormone Ligand Analogues in Humanized PTH1R Knockin Mice

    receptor-binding and signaling potencies for such ligands when assessed on rodent vs human PTH1Rs, as shown by cell-based Expression is directed by the endogenous mouse promoter and hence occurs in all biologically relevant cells PTH analog peptides that are consistent with a fully functional human PTH1R in target bone and kidney cells

  • Structures of oxysterol sensor EBI2/GPR183, a key regulator of the immune response

    August 2022 "Oxysterols induce the migration of B-lymphocytes and dendritic cells to interfollicular oxysterol binding site and the Gαi interface attenuate G protein signaling and abolish oxysterol-mediated cell

  • Dr. GPCR University registration is now open! Secure your spot now!

    Mechanisms of action for new GPCR ligands Essentials for effective GPCR discovery Discover: Advanced cellular Genomic Activity of an EP4-Receptor and β2-Adrenoceptor Agonist in BEAS-2B Human Bronchial Epithelial Cells Mechanistic insights from the atomic-level quaternary structure of short-lived GPCR oligomers in live cells November 5 - 7, 2024 | 16th Annual PEGS Europe September 18, 2024 | FREE Webinar - The value of GPCR cell-based

  • Endosomal parathyroid hormone receptor signaling

    assumes that stimulation of heterotrimeric G protein signaling upon ligand binding occurs solely at the cell Internalized receptors can then recycle back to the cell surface or be trafficked to lysosomes for degradation

  • The regulation of PKA signaling in obesity and in the maintenance of metabolic health

    October 2022 "The cAMP-dependent protein kinase (PKA) system represents a primary cell-signaling pathway control of synaptic events, exocytosis, transcriptional regulation, and more, PKA signaling regulates cellular Neural PKA signaling is regulated by afferent and peripheral efferent signals that link specific neural cell

  • β-arrestin1 and 2 exhibit distinct phosphorylation-dependent conformations when coupling to the...

    2 exhibit distinct phosphorylation-dependent conformations when coupling to the same GPCR in living cells Employing GRK knockout cells and β-arrestins lacking the finger-loop-region, we show that the two isoforms

  • The Adhesion GPCR VLGR1/ADGRV1 Regulates the Ca2+ Homeostasis at Mitochondria-Associated ER Membrane

    associated with the human Usher syndrome (USH), the most common form of inherited deaf-blindness as well interactome of VLGR1, molecules are enriched that are associated with both the ER and mitochondria, as well proteins of MAMs by pull-down assays in vitro complemented by in situ proximity ligation assays in cells The absence of VLGR1 in tissues and cells derived from VLGR1-deficient mouse models resulted in alterations

  • 📰 GPCR Weekly News, March 27 to April 4, 2023

    Statins inhibit protein kinase D (PKD) activation in intestinal cells and prevent PKD1-induced growth Dietary compounds activate an insect gustatory receptor on enteroendocrine cells to elicit myosuppressin A Vaccinia-based system for directed evolution of GPCRs in mammalian cells. FREE Symposium - IPI Surfacing (June 15, 2023) Training School on “Cell-based assays to study Adhesion Meeting IRN I-GPCRNet (October 25 - 27, 2023) GPCR Jobs NEW Convergent Research - Senior Scientist, Cell-Based

  • Microbial Metabolites Orchestrate a Distinct Multi-Tiered Regulatory Network in the Intestinal Epith

    RNA sequencing of intestinal epithelial cells responding to butyrate and secondary bile acids in combination Using shRNA knockdown and CRISPR/Cas9 knockout cell lines, as well as mouse models, we confirmed the

  • A broad look into the future of systemic sclerosis

    Fibroblasts are a key effector cell in SSc. Promising nuclear receptors as key regulators of transcriptional programmes will be introduced as well Fibroblasts from SSc patients exhibit a specific signalling and reactivate developmental pathways and stem cell

  • Unlocking the Future of Medicine: Advancements in GPCR Research

    Selection Dates: October 3rd to 31st, 2024 Registrants will learn: The powerful applications of new cellular underlying atypical chemokine receptor 3 activation RhoA-mediated G12-G13 signaling maintains muscle stem cell quiescence and prevents stem cell loss Activation of Polycystin-1 Signaling by Binding of Stalk-derived November 5 - 7, 2024 | 16th Annual PEGS Europe September 18, 2024 | FREE Webinar - The value of GPCR cell-based Senior Scientist - Discovery Biotherapeutics Post-Doctoral Fellow/Trainee Scientist; Biochemistry, Cellular

  • Cholesterol-Dependent Dynamics of the Serotonin1A Receptor Utilizing Single Particle Tracking: ...

    Particle Tracking: Analysis of Diffusion Modes "G protein-coupled receptors (GPCRs) are signaling hubs in cell Notably, in cholesterol-depleted cells, we observed an increase in the confined population of the receptor

  • Unlocking the Therapeutic Potential of Previously Undruggable GPCRs

    of several hundred candidate hits, enriched because they either bind with increased affinity at the cell Figure 4: Payload delivery using Orion’s superagonist analogs on target cells expressing CCR5. at killing cells than unconjugated MMAE, the Orion CCL5 superagonist analog significantly increased both the potency and selectivity of the payload towards target cells. Figure 5: In an in vitro functional signaling assay on a human monocytic cell line, OB-004 demonstrated

  • Cholesterol-Dependent Dynamics of the Serotonin 1A Receptor Utilizing Single Particle Tracking....

    September 2022 "G protein-coupled receptors (GPCRs) are signaling hubs in cell membranes that regulate Notably, in cholesterol-depleted cells, we observed an increase in the confined population of the receptor

  • Melatonin MT 2 receptor is expressed and potentiates contraction in human airway smooth muscle

    ([Ca2+]i), which modulate airway smooth muscle tone; and whether they promote airway smooth muscle cell in native human and guinea pig airway smooth muscle and cultured human airway smooth muscle (HASM) cells MT2 agonist ramelteon (10 µM) significantly inhibited forskolin-stimulated cAMP accumulation in HASM cells acetylcholine-stimulated [Ca2+]i increases, stress fiber formation through the MT2 receptor in HASM cells

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