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311 items found for "cell biology"
- Endosomal parathyroid hormone receptor signaling
assumes that stimulation of heterotrimeric G protein signaling upon ligand binding occurs solely at the cell Internalized receptors can then recycle back to the cell surface or be trafficked to lysosomes for degradation
- The regulation of PKA signaling in obesity and in the maintenance of metabolic health
October 2022 "The cAMP-dependent protein kinase (PKA) system represents a primary cell-signaling pathway Neural PKA signaling is regulated by afferent and peripheral efferent signals that link specific neural cell
- Melatonin MT 2 receptor is expressed and potentiates contraction in human airway smooth muscle
([Ca2+]i), which modulate airway smooth muscle tone; and whether they promote airway smooth muscle cell in native human and guinea pig airway smooth muscle and cultured human airway smooth muscle (HASM) cells MT2 agonist ramelteon (10 µM) significantly inhibited forskolin-stimulated cAMP accumulation in HASM cells acetylcholine-stimulated [Ca2+]i increases, stress fiber formation through the MT2 receptor in HASM cells
- β-arrestin1 and 2 exhibit distinct phosphorylation-dependent conformations when coupling to the...
2 exhibit distinct phosphorylation-dependent conformations when coupling to the same GPCR in living cells Employing GRK knockout cells and β-arrestins lacking the finger-loop-region, we show that the two isoforms
- The Adhesion GPCR VLGR1/ADGRV1 Regulates the Ca2+ Homeostasis at Mitochondria-Associated ER Membrane
associated with the human Usher syndrome (USH), the most common form of inherited deaf-blindness as well interactome of VLGR1, molecules are enriched that are associated with both the ER and mitochondria, as well proteins of MAMs by pull-down assays in vitro complemented by in situ proximity ligation assays in cells The absence of VLGR1 in tissues and cells derived from VLGR1-deficient mouse models resulted in alterations
- N-Acyl Amides from Neisseria meningitidis and Their Role in Sphingosine Receptor Signaling
Both groups of metabolites suppress anti-inflammatory interleukin-10 signaling in human macrophage cell they also suppress the pro-inflammatory interleukin-17A+ population in TH 17-differentiated CD4+ T cells
- 📰 GPCR Weekly News, March 11 to 17, 2024
The Prostaglandin E2 EP3 Receptor Has Disparate Effects on Islet Insulin Secretion and Content in β-cells Time-resolved cryo-EM of G-protein activation by a GPCR Industry News Domain Therapeutics and Chime Biologics Phosphorylation and G-Protein Mediated Signaling Networks June 25 - 29, 2024 | FENS Forum 2024 October 2024 | Biologics
- Targeted Activation of G-Protein Coupled Receptor-Mediated Ca 2+ Signaling Drives Enhanced Cartilage
processes that govern chondrocyte gene expression, protein synthesis, cytoskeletal remodeling, and cell synthetically initiate robust [Ca2+]i transients and oscillatory [Ca2+]i signaling in chondrocyte-like ATDC5 cells under repeated CNO stimulation modified the temporal dynamics of hM3Dq [Ca2+]i signaling, increased cell enhanced matrix production in a CNO dose- and frequency-dependent manner, and triggered the formation of cell
- 📰 GPCR Weekly News, June 12 to 18, 2023
GPCRs in Oncology and Immunology The GPCR-Gαs-PKA signaling axis promotes T cell dysfunction and cancer induces IFN-γ but suppresses IL-2 production by inhibiting activation of pAKT pathways in primary T cells zoominar (June 22, 2023) NEW Meet Inoviem Scientific Team (June 19 - 23, 2023) Training School on “Cell-based
- A broad look into the future of systemic sclerosis
Fibroblasts are a key effector cell in SSc. Promising nuclear receptors as key regulators of transcriptional programmes will be introduced as well Fibroblasts from SSc patients exhibit a specific signalling and reactivate developmental pathways and stem cell
- Microbial Metabolites Orchestrate a Distinct Multi-Tiered Regulatory Network in the Intestinal Epith
RNA sequencing of intestinal epithelial cells responding to butyrate and secondary bile acids in combination Using shRNA knockdown and CRISPR/Cas9 knockout cell lines, as well as mouse models, we confirmed the
- VAMP2: a crucial player in the delivery of MOR to the synapse
complex, including vesicle-associated membrane protein 2 (VAMP2), regulate this last step toward the cell characterized, the mechanisms that modulate the delivery of GPCRs to the membrane cell still need to be well known [3-5]. in regulating dopamine D2 receptor signaling by controlling the trafficking of the receptor to the cell presence of VAMP2, specifically in recycling vesicles containing MOR but not B2AR or TFR in HEK293 cells
- Cholesterol-Dependent Dynamics of the Serotonin1A Receptor Utilizing Single Particle Tracking: ...
Particle Tracking: Analysis of Diffusion Modes "G protein-coupled receptors (GPCRs) are signaling hubs in cell Notably, in cholesterol-depleted cells, we observed an increase in the confined population of the receptor
- Cholesterol-Dependent Dynamics of the Serotonin 1A Receptor Utilizing Single Particle Tracking....
September 2022 "G protein-coupled receptors (GPCRs) are signaling hubs in cell membranes that regulate Notably, in cholesterol-depleted cells, we observed an increase in the confined population of the receptor
- GPR84 signaling promotes intestinal mucosal inflammation via enhancing NLRP3 inflammasome activation
medium-chain free fatty acid receptor GPR84 is a G protein-coupled receptor primarily expressed in myeloid cells These results define a unique role of GPR84 in innate immune cells and intestinal inflammation, and suggest
- Gαs and Gαq/11 protein coupling bias of two AVPR2 mutants (R68W and V162A) that cause nephrogenic di
protein coupled receptor (GPCR) and mainly couples with Gαs protein leading to cAMP accumulation in the cell showed the ability to couple with Gαq/11 protein and make Ca2+ mobilization at different levels in the cell
- Pharmacological targeting of cGAS/STING-YAP axis suppresses pathological angiogenesis and...
showed that cGAS expression was induced in fibrotic liver and kidney, but suppressed in endothelial cells Meanwhile, cGAS deletion upregulated profibrotic Yes-associated protein (YAP) signaling in endothelial cells
- GPR15 expressed in T lymphocytes from RA patients is involved in leukocyte chemotaxis to the...
the synovium "The rheumatoid arthritis (RA) inflammatory process occurs in the joints where immune cells evaluated the expression of GPR15 and GPR15L in blood and synovial tissue samples from RA patients, as well synovial fluid and peripheral blood were analyzed for CD45+CD3+CD4+GPR15+ and CD45+CD3+CD8+GPR15+ T cell
- 📰 GPCR Weekly News, February 12 to 18, 2024
Endocrinology, and Taste The Role of G Protein-Coupled Receptors and Receptor Kinases in Pancreatic β-Cell Operations - Single-molecule Imaging Center Scientific Assistant Postdoctoral Fellowship- Mechanistic Biology
- G protein-coupled receptor kinase 2 is essential to enable vasoconstrictor-mediated arterial ...
increases arterial contraction and stimulates signalling pathways that promote vascular smooth muscle cell Using two indices of cell growth, we show that PI3K inhibition and depletion of GRK2 expression produced ability to promote efficient prolonged PI3K-Akt signalling, and thus relieve the GSK3-mediated block on cell
- Regulation of rod photoreceptor function by farnesylated G-protein γ-subunits
G-protein transducin, Gt, is a key signal transducer and amplifier in retinal rod and cone photoreceptor cells Immunohistochemistry and Western blotting demonstrated the robust expression of each transgenic Gγ in rod cells
- Diversity and specificity in location-based signaling outputs of neuronal GPCRs
of the G protein-coupled receptor (GPCR) family respond to neurotransmitters in the brain have been well GPCRs, and how these could interact to generate specific spatiotemporal patterns of GPCR signaling in cells
- Constitutive, Basal, and β-Alanine-Mediated Activation of the Human Mas-Related G Protein-Coupled ..
Several members of MRGPRs are also expressed in mast cells, macrophages, and in cardiovascular tissue By stimulating human MRGPRD-expressing HeLa cells with the agonist β-alanine, we observed a release of as an assay for β-alanine-mediated activation of MRGPRD is substantially increased by culturing the cells
- Jan Steyaert Named 2022 Jacob and Louise Gabbay Award Winner
.- Jan Steyaert , scientific director of the VIB-VUB Center for Structural Biology , Vlaams Instituut mechanistic understanding of one of the biggest classes of pharmaceutical targets by combining structural biology
- Deficiency of β-arrestin2 alleviates apoptosis through GRP78-ATF6-CHOP signaling pathway in ...
In vitro, we used IFNα to stimulate human salivary gland epithelial cells (HSGECs), and the results showed that IFNα activated GRP78-ATF6-CHOP apoptosis signaling, decreased cell viability, and induced apoptosis In addition, β-arrestin2 depletion downregulated GRP78-ATF6-CHOP apoptosis signaling to alleviate cell
- Novel Therapies for Cardiometabolic Disease: Recent Findings in Studies with Hormone...
One successful strategy is the so-called metabolic surgery whose beneficial effects are beyond dietary The present review describes novel findings regarding the complex biology of the preproglucagon-derived
- A cryptic mode of GPCR regulation revealed
In a recent issue of Molecular Cell, Fonseca et al. identified a previously overlooked desensitization
- The integrin ligand SVEP1 regulates GPCR-mediated vasoconstriction via integrins α9β1 and α4β1
and purpose: Vascular tone is regulated by the relative contractile state of vascular smooth muscle cells