Characterization of a new WHIM syndrome mutant reveals mechanistic differences in regulation of the chemokine truncations, frame shifts and point mutations in the chemokine receptor CXCR4 have been identified in WHIM Here, we report on a new WHIM syndrome mutation that results in a frame shift within the codon for Ser339 (S339fs5) and compare the properties of S339fs5 with wild type CXCR4 and a previously identified WHIM Taken together, these studies identify a new WHIM syndrome mutant, CXCR4-S339fs5, that promotes enhanced

N7.49P7.50xxY7.53 and D3.49R3.50Y3.51 motifs, located near the intracellular ends of TM7 and TM3, respectively (Weis and the intracellular helix 8 move outward from the receptor core to make room for the G-protein (Lu, Zhao namely CCL15L (residues 26-92) and CCL15M (residues 27-92), while the third structure lacks a ligand (Shao conformational change of Y2917.43 tilted toward TM2, a model supported by mutagenesis experiments (Shao

For complete details on the use and execution of this protocol, please refer to Wei et al. (2022) and

Authors Yanni Xi, Zhenya Min, Mianxue Liu, Xueqin Li, Zhao-Hua Yuan Tags P2Y12R , P2Y12R inhibitors ,

exploration of therapeutic modalities in G-protein signaling that go beyond targeting GPCRs. " Authors Jingyi Zhao

Authors Miao Guo , Yan Zhang , Lehao Wu , Ye Sheng , Jiaqi Zhao , Zeyuan Wang , Huiyu Wang , Lu Zhang