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290 items found for " double mutant cycle analysis"
Posts (184)
- Cell Surface Calcium-Sensing Receptor Heterodimers: Mutant Gene Dosage Affects Ca 2+ Sensing but...
September 2022 Cell Surface Calcium-Sensing Receptor Heterodimers: Mutant Gene Dosage Affects Ca 2+ Sensing CaSR-GABAB1 and CaSR-GABAB2 chimeras subject to GABAB -dependent endoplasmic reticulum sorting to traffic mutant Transfected HEK-293 cells were assessed for Ca2+o -stimulated Ca2+i mobilization using mutations in either When the same mutation was present in both VFT domains of receptor dimers, analogous to homozygous neonatal Mutant heterodimers containing one wild-type (WT) and one mutant VFT domain, however, corresponding to
- Characterization of a new WHIM syndrome mutant reveals mechanistic differences in regulation of ...
Characterization of a new WHIM syndrome mutant reveals mechanistic differences in regulation of the chemokine and compare the properties of S339fs5 with wild type CXCR4 and a previously identified WHIM syndrome mutant The S339fs5 and R334X mutants exhibited significantly increased signaling compared to wild type CXCR4 effectively inhibited by the CXCR4 antagonist TE-14016, this had no effect on the degradation of the WHIM mutants Taken together, these studies identify a new WHIM syndrome mutant, CXCR4-S339fs5, that promotes enhanced
- Rescue of Cell Surface Expression and Signaling of Mutant Follicle-Stimulating Hormone Receptors
Mutations in G protein-coupled receptors (GPCRs) underlie numerous diseases. Pharmacological chaperones are cell-permeant small molecules that engage nascent mutant GPCRs in the We previously demonstrated rescue of cell surface expression of luteinizing hormone receptor mutants Here we demonstrate that a similar approach can be employed to rescue mutant follicle-stimulating hormone acts as a pharmacological chaperone and can rescue cell surface expression and function of certain mutant
Other Pages (105)
- Stable Binding of Full-Length Chemerin is Driven by Negative Charges in the CMKLR1 N-terminus
Tags double mutant cycle analysis , protein expression , protein-protein interaction , receptor binding
- Intermediate-state-trapped mutants pinpoint G protein-coupled receptor conformational allostery
< GPCR News < GPCRs in Oncology and Immunology Intermediate-state-trapped mutants pinpoint G protein-coupled demonstrate the feasibility of enriching the populations of discrete states via conformation-biased mutants These mutants adopt distinct distributions among five states that lie along the activation pathway of Intermediate-state-trapped mutants will also provide useful information in relation to receptor-G protein
- Functional Assessment of Cancer-Linked Mutations in Sensitive Regions of Regulators of G Protein Signaling Predicted by Three-Dimensional Missense Tolerance Ratio Analysis
Regions of Regulators of G Protein Signaling Predicted by Three-Dimensional Missense Tolerance Ratio Analysis Here we analyze the RGS domains of 15 RGS protein family members using a novel bioinformatic tool that Subsequent permutation analysis can define the protein regions that are most significantly intolerant , including cancer-linked mutations in regulator of G protein signaling (RGS) proteins. (3DMTR) to define regions of genetic intolerance in RGS proteins and prioritize which cancer-linked mutants