Biosensors for monitoring G protein-coupled receptors (GPCRs), the most drugged class of proteins in Their applications have continually expanded our understanding of this important protein class.

October 2022 Mechanistic Understanding of the Palmitoylation of Go Protein in the Allosteric Regulation of Adhesion Receptor GPR97 "Adhesion G-protein-coupled receptors (aGPCRs)-a major family of GPCRs-play Although it has been established that the palmitoylation of the C-terminal Go protein is essential for overall conformation of GPR97 is preferred to be fully active when interacting with palmitoylated Go protein provides mechanistic insights into the regulation of aGPCRs via post-translational modifications of the Go protein

cryogenic-electron microscopy (cryo-EM) is used extensively to determine structures of activated G protein-coupled receptors (GPCRs) in complex with G proteins or arrestins. However, applying it to GPCRs without signaling proteins remains challenging because most receptors lack In GPCR crystallography, inserting a fusion protein between transmembrane helices 5 and 6 is a highly Here, we address this shortcoming by exploring different fusion protein designs, which lead to structures

Here, we find that GPR143, an atypical GPCR, controls the endosomal sorting complex required for the EGFR, which subsequently enables selective protein sorting into intraluminal vesicles (ILVs) in multivesicular cargo, including integrins signaling proteins. These findings provide a mechanism for regulating the exosomal proteome and demonstrate its ability to protein sorting , exosome biogenesis , melanoma , metastasis .

< GPCR News < GPCRs in Oncology and Immunology G protein-coupled estrogen receptor (GPER)/GPR30 forms a complex with the β1-adrenergic receptor, a membrane-associated guanylate kinase (MAGUK) scaffold protein , and protein kinase A anchoring protein (AKAP) 5 in MCF7 breast cancer cells Published date January 10, 2024 Abstract "G protein-coupled receptor 30 (GPR30), also named G protein-coupled estrogen receptor , and in turn protein kinase A anchoring protein (AKAP) 5.

Oncology and Immunology Molecular docking and dynamics simulation studies uncover the host-pathogen protein-protein of P. vannamei with V. parahaemolyticus proteins associated with virulence factors. The P. vannamei-V. parahaemolyticus protein-protein interaction of Complex 1 (Ferritin-HrpE/YscL family type III secretion apparatus protein), Complex 2 (Protein kinase domain-containing protein-Chemotaxis CheY protein), and Complex 3 (GPCR-Chemotaxis CheY protein) was found to interact with -4319.76, -5271.39