August 2022 GPR3 expression in retinal ganglion cells contributes to neuron survival and accelerates Our earlier reports suggested that GPR3 enhances both neurite outgrowth and neuronal survival. neurons from the retina showed that GPR3 expression was correlated with neurite outgrowth and neuronal survival These results suggest that GPR3 expression in RGCs helps maintain neuronal survival and accelerates axonal

protein-coupled receptors as potential therapeutic targets for adult diffuse gliomas: a systematic analysis Moreover, through systematic survival analysis on OR genes, OR51E1 (mouse Olfr558) was identified as biomarker of favorable overall survival in isocitrate dehydrogenase (IDH) wild-type glioma. In addition to transcriptomic analysis, mutational profiles revealed that somatic mutations in OR genes Based on this systematic analysis and review of the genomic and transcriptomic profiles of ORs in glioma

October 2022 Design and validation of recombinant protein standards for quantitative Western blot analysis Replacement of radioligand binding assays with antibody-antigen interaction-based approaches for quantitative analysis This renders full-length recombinant GPCRs useless for analytical purposes, a problem that can be overcome C-terminal tail for use as standard and negative control, respectively, in quantitative Western blot analysis

Immunology Identification of a G-protein coupled receptor-related gene signature through bioinformatics analysis GPCRRGs were screened using least absolute shrinkage and selection operator (LASSO) Cox regression analysis The predictive ability of the model was evaluated by Kaplan-Meier (K-M) survival analysis. gene set enrichment analysis (ssGSEA) and CIBERSORT. K-M analysis of the prognostic model showed that it could differentiate high- and low-risk patients,

partners across cancer subtypes and are associated to specific transcriptional programs and to patient survival cell lines , cell-cell communication , drug repurposing , personalized medicine , signaling network , survival analysis , transcriptomics .

< GPCR News < GPCRs in Oncology and Immunology Comparative Analysis of the GNAI Family Genes in Glioblastoma 2023 Abstract Glioblastoma multiforme (GBM) is one of the most aggressive cancers with a low overall survival MetaCore and gene ontology (GO) analyses were conducted to explore the role of GNAI3 in co-expressed genes and associated signaling pathways using a transcript analysis. A single-cell analysis was used to assess GNAI3 expression in GBM.