We analyzed the gene expression profiles of PR-low and control T-47D cells in the absence of hormone Differentially expressed (DE) genes between experimental groups were characterized based on RNA-seq and Gene Ontology (GO) enrichment analyses. Results: The overall gene expression pattern was very similar between untreated PR-low and untreated expression , Progesterone receptor.

the identification of unique GPCRs expressed in cancer biopsies, matched with personalised GPCR-targeted Here we report on the systematic analysis of public ribonucleic acid-sequencing (RNA-seq) gene expression ovarian cancer cells derived from ascites and ovarian cancer cell lines were used to confirm frequent gene expression for the selected GPCRs. However, the expression levels showed high variability within our selection of samples, therefore, supporting

nematode hunting Published date June 14, 2024 Abstract "Initiation of development requires differential gene expression and metabolic adaptations. Gene-expression analyses revealed that ascarosides activate the fungal GPCR, GprC, at the plasma membrane

A 10-gene expression signature, predictive of response to ONV + DAC, was derived from the leading-edge genes of gene set enrichment analyses (GSEA). The gene signature was evaluated in independent datasets and used to identify associated mutated genes The gene signature was not associated with response to DAC alone in an independent dataset. expression and SF mutations."

In gene expression analysis of mouse lungs with induced fibrosis, eight GPCRs were identified, showing a >2-fold increase in mRNA expression after fibrosis induction. (αSMA), the profibrotic factor transforming growth factor β1 (TGFβ1), and collagen in a human lung gene expression database. Furthermore, fibroblasts abundantly expressed Gpr176 compared to alveolar epithelial cells, endothelial

We used The Cancer Genome Atlas (TCGA) GBM genomic dataset to identify differentially expressed genes Within this dataset, we observed the association in the tumor microenvironment between the gene expression MetaCore and gene ontology (GO) analyses were conducted to explore the role of GNAI3 in co-expressed genes and associated signaling pathways using a transcript analysis. A single-cell analysis was used to assess GNAI3 expression in GBM.

After 2-24 h, brains were collected for the analysis of gene expression by RT-PCR or protein expression with 25 mM ethanol for 4 h and then with LPS (100 ng/ml) followed by 10 nM synaptamide for 2 h for gene expression and 12 h for protein analysis. The LPS-induced Iba-1 expression in the brain was significantly higher after ethanol pretreatment in while synaptamide suppressed its expression in a GPR110-dependent manner.

beta-adrenergic receptors localized to the inner nuclear membrane and their role in the regulation of gene expression."

GPCR News < GPCRs in Oncology and Immunology Identification of a G-protein coupled receptor-related gene Method: We downloaded data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases The immunological characteristics of the high- and low-risk groups were analyzed using single-sample gene CXCR4, GPR34, LGR6, LPAR3, and RGS2 were significantly expressed in three OV datasets and enabled accurate GPCRRG expression was correlated with immune infiltration rates.

Immunology [Inhibitory effect of downregulating G protein-coupled receptor class C group 5 member A expression The expressions of GPRC5A of the two groups were detected by immunohistochemistry staining. RT-qPCR was used to detect the gene expression levels of the inflammatory cytokines in GFs induced by Meanwhile, RT-qPCR results showed that the gene expression levels of GPRC5A at different time point ( RT-qPCR results showed that the expressions of IL-8, IL-1β, IL-6, TNF-α, and PTGS2 at the gene level

proteins intracellularly to influence metabolism, oxidative response, epigenetic modification, and gene expression and to signal extracellularly through binding the G protein-coupled receptor (GPCR).

By examining the expression of known and novel marker genes, we validated the identity of these clusters and characterized their gene expression profiles. We found that each cell type could be characterized by a unique expression profile of ion channels, GPCR

< GPCR News < GPCRs in Oncology and Immunology High expression of GPR50 promotes the proliferation, migration To detect the role and the regulation mechanism of GPR50 in HCC, GPR50 expression was analyzed in HCC patients (gene expression omnibus database (GEO) (GSE45436)) and detected in HCC cell line CBRH-7919

GPCRs in Oncology and Immunology Drosophila cytokine GBP2 exerts immune responses and regulates GBP1 expression SRP gene expression has been also demonstrated to be enhanced by GBP, indicating that both cytokines Moreover, the GBP2-induced response was silenced by pre-treatment with dsRNA targeting the GBP receptor gene GBP2 expression. GBP2-induced enhancement of GBP1 expression was not observed in Mthl10 knockdown cells.

These patients also exhibited upregulated expression of most metabolite-sensing GPCRs analysed, which The role of GPR109A was studied in depth and increased expression of this receptor was detected in epithelial The treatment with β-hydroxybutyrate increased gene expression of GPR109A, CD86, IL1B and NOS2 in U937 Besides, when GPR109A was transiently silenced, the mRNA expression and secretion of IL-1β, IL-6 and Finally, the secretome from siGPR109A M1 macrophages reduced the gene and protein expression of COL1A1

< GPCR News < GPCRs in Oncology and Immunology Expression pattern and clinical significance of beta 2 The present study aimed at investigating the β2-AR gene expression and its significance in relation with Results: Out of the total of 65 OSCC tissues, 41 tissues (63.1%) exhibited high expression for β2-AR High β2-AR protein expression was significantly associated with multiple risk habits (p = 0.045), histological

, studies predicting therapeutic drug targets for cancer therapy focused on the assumption that one gene Therefore, an inactive rhomboid protease known as iRhom2 encoded by the gene RHBDF2 can be considered Speculatively, previous studies on gene expression analysis of RHBDF2 showed heterogenous behaviour during

Alterations in GPCR gene expression and dysregulation of signal transduction have been recognized as unknown functions in tumors, particularly in non-small-cell lung cancer (NSCLC) METHODS: We explored the expression We detected the expression of GPR37 in NSCLC tissues and cell lines. Results: (1) In NSCLC, the expression of GPR37 is markedly higher than that in corresponding normal tissues We found that elevated GPR37 expression predicts an unfavorable prognosis. (2) It was demonstrated that

< GPCR News < GPCRs in Oncology and Immunology GPR37 expression as a prognostic marker in gliomas: a The aim of this study was to explore the relationship between GPR37 gene expression and the clinicopathological Results: GPR37 expression was significantly higher in the glioma tissues compared to the normal brain TIMER2.0 and ssGSEA showed that GPR37 expression was significantly associated with the infiltration of Finally, hypomethylation of the GPR37 promoter was associated with its high expression levels and poor

Through study of known protein isoforms of CELSR1, which would be missed in gene expression microarray in the collecting duct and TAL of the loop of Henle with limited expression in the proximal tubule. expression assays. and protein expression. RNAseq data indicated significantly impaired developmental gene expression for neuronal differentiation

expression in pancreatic cancer (Feigin and Garvin, et al., Nature Genetics, 2017). Mike expressed his initial reluctance due to a lack of confidence and fear of not being ready. The conversation ended with Yamina expressing interest in learning more about Mike's two main research They also discussed the challenges they face in studying GPCRs due to their low expression levels and Mike expressed a need for better tools to study GPCR localization in tissues.

Immunology Exploration of prognostic and treatment markers in hepatocellular carcinoma via GPCR-related genes shrinkage and selection operator (LASSO) were performed with the aim of identifying differentially expressed GPCR-related genes and grouping patients. Differential expression and functional enrichment analyses were performed; protein-protein interaction (PPI) mechanisms were explored; hub genes and micro ribonucleic acid (miRNA)-target gene regulatory

Oncology and Immunology Evolutionary diversity of CXCL16-CXCR6: Convergent substitutions and recurrent gene Previous studies of the CXCL16-CXCR6 axis found genetic variation among species but were limited to primates The unique DRF motif of CXCR6 facilitates leukocyte adhesion by interacting with cell surface-expressed We establish recurrent CXCR6 gene loss in 10 out of 36 bird orders, including Galliformes and Passeriformes Salve, Sandhya Sharma, Nagarjun Vijay Tags ITGAE , Chemokine receptors , DRY/DRF/DRL motif , GC-rich gene

< GPCR News < GPCRs in Oncology and Immunology S1P Signaling Genes as Prominent Drivers of BCR-ABL1-Independent This study investigated the sphingosine-1-phosphate (S1P) signaling-associated genes (SphK1 and S1PRs transcriptomic analysis of IM-sensitive and IM-resistant CML groups, we identified the differentially expressed genes and found a notable upregulation of SphK1, S1PR2, and S1PR5 in IM-resistant CML. Further, we identified interactors such as BIRC3, TRAF6, and SRC genes, with potential implications for

Second, the differential regulation of the gene network between the sympathetic-adrenal medullary axis There was no difference in adrenocorticotropic hormone expression between any of the groups. The transcriptome analysis results showed that the downregulated differentially expressed genes (DEGs The upregulated genes in the adrenal glands (hub genes: Ciart, per2, per3, cry1, and cry2) were enriched in cortisol secretion and circadian rhythm changes, and the downregulated genes (hub genes: IL7r, rt1

Regulator of G protein signaling protein 6 (RGS6), identified as a tumor suppressor gene, has received Methods: Congruously regulated G protein-coupled receptor (GPCR)-related genes and differentially expressed immunofluorescence staining, and western blotting were used to determine the dynamic changes in RGS6 expression genes) were identified among GPCR-related genes and DEGs in the ALI model. RGS6 was expressed in the cytoplasm and accumulated in the nucleus after LPS stimulation.

The resulted raw data were analyzed by conventional RNA-Seq software to determine the Differentially Expressed Genes (DEGs). Afterward, Gene Ontology (GO) and pathway enrichment analyses were conducted to determine the enriched Eventually, the relative expressions of some significant genes were validated in the twenty external The expression levels of six genes involved in enriched pathways including F3, PTX3, ADRA2B, GNG11, GP9

PARs are highly expressed in many cancer cells, including prostate cancer (PCa), and regulate various , we examined the androgen-independent human prostatic cancer cell line PC3 and find the functional expression Using genetically encoded PAR cleavage biosensors, we showed that PC3 cells secrete proteolytic enzymes CRISPR/Cas9 targeting of PAR1 and PAR2 combined with microarray analysis revealed genes that are regulated We found several genes that are known PCa prognostic factors or biomarker to be differentially expressed