September 2022 β2-Adrenergic Receptor Expression and Intracellular Signaling in B Cells Are Highly Dynamic In the past, treatment of arthritic B cells with a β2-adrenergic receptor (β2-ADR) agonist has been shown In this study, the expression and signaling of β2-ADR in B cells during collagen-induced arthritis (CIA Splenic B cells were isolated via magnetic-activated cell sorting (MACS). B cells increase during CIA without a change in receptor density.

Canonical chemokine receptors – scavenging “decoys” Chemokine receptors coordinate cell migration upon migration. CCR2 is an example of a dual-function receptor that directly regulates both cell migration and scavenging Scavenging may allow cells to continuously migrate by remaining responsive to chemokines (S. migration (A.

Data by Arne Hansen et a l has introduced a sensitive method for detecting GPCR mRNAs in human immune cells The study reports that human white blood cells express an average of 160 GPCR mRNAs, ranging from 123 , suggesting a potential role in regulating red blood cell production and offering a novel avenue for Additionally, the study identified unique expression patterns of GPCRs in different immune cell types , including B cells, CD4+ T cells, CD8+ T cells, natural killer (NK) cells, and dendritic cells.

into studies focused on obtaining detailed molecular information in the intracellular context ("in-cell ") or those focused on characterizing molecules or events at the cell surface ("on-cell"). In this review, we outline some key NMR techniques applied for on-cell NMR studies through both solution with cell surface membrane proteins such as G-protein coupled receptors (GPCRs) and receptor tyrosine We also provide a brief survey of the applicability of on-cell NMR approaches to other classes of cell

One suggested theory is that cannabinoids regulate a variety of physiological processes within the cells Murine L6 skeletal muscle cells were used in order to clarify additional possible molecular signaling Skeletal muscle cells have often been used; it is well-documented that they express cannabinoid receptors In the current work, it is demonstrated that skeletal muscle cells exhibit functional expression of CB1 Thus, in skeletal muscle cells, a possible physiological expression of CB1 receptors was identified.

August 2022 Deciphering the signaling mechanisms of β-arrestin1 and β-arrestin2 in regulation of cancer cell They have participated in various cellular processes such as cell proliferation, migration, apoptosis

Here, we provide evidence that activated human T cells express GLP-1R. Analysis of CD4+ T cells activated under T helper (Th) 1, Th2, Th17 and regulatory T (Treg) cell differentiation FoxP3+CD25+CD127- iTreg cells. GLP-1R in human CD4+ T cells. Given the high induction of GLP-1R in human iTreg cells, we hypothesize that GLP-1R+ iTreg cells play

August 2022 TAS2R supports odontoblastic differentiation of human dental pulp stem cells in the inflammatory Background: Inflammatory microenvironment promotes odontoblastic differentiation in human dental pulp stem cells

The hepatitis B virus X protein (HBx) is involved in the process of hepatocellular carcinoma via the Inhibition of autophagy by 3-methyladenine or interference of ATG5 or ATG7 attenuated HBx-induced cell protein-coupled receptors; GST: glutathione S-transferase; HCC: hepatocellular carcinoma; HBV: hepatitis B virus; HBx: hepatitis B virus X protein; HMGB1: high mobility group box 1; HIF1A/HIF-1α: hypoxia inducible kinase; MAPK: mitogen-activated protein kinase; 3-MA: 3-methyladenine; NFKB/NF-κB: nuclear factor kappa B;

August 2022 Luciferase-based GloSensor™ cAMP assay: Temperature optimization and application to cell-based Monitoring intracellular cAMP levels can help identify orthosteric agonists and antagonists, as well As well, it has not been reported whether the effects of temperature on biosensor activity are reversible We also present a detailed protocol for monitoring cAMP levels in live cells expressing endogenous or Temperature optimization studies were carried out using HEK293H cells transiently transfected with the

October 2022 "Study of small molecule binding to live cells provides important information on the characterization Here we developed and validated a label-free, liquid chromatography-mass spectrometry (LC-MS) based cell This versatile method allows quantitative characterization of ligand binding to cell surface expressed

September 2022 Cell-Type-Specific Effects of the Ovarian Cancer G-Protein Coupled Receptor (OGR1) on However, in epithelial cells, OGR1 promotes epithelial to mesenchymal transition (EMT) and inflammation localization and alternative signaling pathways may be responsible for the differential effect of OGR1 in each cell

At the cellular level, the plasmatic membrane is a thin layer of lipids that surrounds the entire cell interplay between GPCRs and β-arrestins and how this complex operates on the plasma membrane of living cells dissected the sequence of events in receptor-β-arrestin interactions at the plasma membrane of living cells Cell, 186(10), 2238–2255.e20. https://doi.org/10.1016/j.cell.2023.04.018 Janetzko, J., Kise, R., Barsi-Rhyne Cell, 185(24), 4560–4573.e19. https://doi.org/10.1016/j.cell.2022.10.018 Latorraca, N.R., Wang, J.K.,

October 2022 Keratinocyte-derived defensins activate neutrophil-specific receptors Mrgpra2a/b to prevent Here, we discovered that the epithelial-cell-derived antimicrobial peptides defensins activated orphan G-protein-coupled receptors (GPCRs) Mrgpra2a/b on neutrophils. generated mutant mouse lines lacking the entire Defensin (Def) gene cluster in keratinocytes or Mrgpra2a/b.

September 2022 Cell Surface Calcium-Sensing Receptor Heterodimers: Mutant Gene Dosage Affects Ca 2+ Sensing chimeras subject to GABAB -dependent endoplasmic reticulum sorting to traffic mutant heterodimers to the cell Transfected HEK-293 cells were assessed for Ca2+o -stimulated Ca2+i mobilization using mutations in either

October 2022 Focusing on the role of secretin/adhesion (Class B) G protein-coupled receptors in placental endoplasmic reticulum stress, dysregulated immune tolerance, vascular inflammation and endothelial cell Among them, the secretin/adhesion (Class B) G protein-coupled receptors are essential drug targets for Given the great value of the secretin/adhesion (Class B) G protein-coupled receptors in the regulation

September 2022 GASP1 enhances malignant phenotypes of breast cancer cells and decreases their response , such as inhibition of cell proliferation, colony formation, migration, invasion and xenograft tumor growth in nude mice as well as induction of G1-phase cell cycle arrest, and vice versa. Besides, we found that GASP1 knockout obviously improved the response of breast cancer cells to paclitaxel Collectively, this study demonstrates that GASP1 enhances malignant behaviors of breast cancer cells

November 2022 Deciphering the signaling mechanisms of β-arrestin1 and β-arrestin2 in regulation of cancer cell They have participated in various cellular processes such as cell proliferation, migration, apoptosis

September 2022 Lack of Oestrogen Receptor Expression in Breast Cancer Cells Does Not Correlate with Kisspeptin Signalling and Migration "Kisspeptin is an anti-metastatic mediator in many cancer types, acting through In KISS1R overexpressing triple-negative breast cancer (TNBC) cells, stimulation has been associated However, KP-10 stimulation induced migration of MDA-MB-231, but not BT-20 cells, in a calcium-dependent Overall, our data shows that, in TNBC, it is not universally true that kisspeptin/KISS1R stimulate migration

histamine receptor 2 (H2R), a G protein-coupled receptor (GPCR), which is expressed in a wide variety of cell study, for the first time, we demonstrate the nuclear localization of H2R in lymphatic endothelial cells

August 2022 GPR3 expression in retinal ganglion cells contributes to neuron survival and accelerates GPR3 was relatively highly expressed in retinal ganglion cells (RGCs).

mammary gland (MG) undergoes remodeling, which involves alveolar differentiation of mammary epithelial cells and gene and protein expression of major milk protein and lipid synthesizing markers. using the HC11 cells hypothesized that THC and CBD will negatively impact the synthesis of milk proteins and lipids, as well as lipid markers in HC11 cells. In conclusion, 10μM THC and CBD altered the differentiation of HC11 cells, in part via the CB2 receptor

September 2022 Opposite Effects of Src Family Kinases on YAP and ERK Activation in Pancreatic Cancer Cells IGF-1 receptors and G protein-coupled (GPCR) signaling systems leading to mitogenic signaling in PDAC cells agonist neurotensin induced rapid activation of Src family of tyrosine kinases (SFKs) within PANC-1 cells by FAK phosphorylation at Tyr576/577 and Tyr861, sensitive biomarkers of SFK activity within intact cells and suppessed the growth of MiaPaCa-2 cells xenografted into the flank of nude mice.

September 2022 Expression pattern and clinical significance of beta 2-adrenergic receptor in oral squamous cell However, the role of β2-AR in oral cancer is not well identified. significance in relation with the clinicopathological features and overall survival of oral squamous cell

class A G-protein coupled receptors (GPCRs) through a conserved binding site, not present in class B For class B GPCRs, previous molecular dynamics (MD) simulation studies have shown PI(4,5)P2 interacting appear to stabilize the inactive conformation of GCGR through a binding site conserved across class B This suggests differences in the regulatory function of PI(4,5)P2 between class A and class B GPCRs."

protein signalling using a G protein activation assay and second messenger assay conducted on living cells

September 2022 A2B Adenosine Receptor Enhances Chemoresistance of Glioblastoma Stem-Like Cells under and aggressive primary brain tumor, characterized by its high chemoresistance and the presence of a cell subpopulation that persists under hypoxic niches, called glioblastoma stem-like cells (GSCs). Cell viability was measured by MTS assays. Cell cycle and apoptosis were determined by flow cytometry.

Many cause receptor misfolding and failure to reach the cell surface. Pharmacological chaperones are cell-permeant small molecules that engage nascent mutant GPCRs in the endoplasmic reticulum, stabilizing folding and "rescuing" cell surface expression. We previously demonstrated rescue of cell surface expression of luteinizing hormone receptor mutants of certain mutant FSHRs with severely reduced cell surface expression.