types (neutrophils, T cells, B cells and dendritic cells) and systematically addressed the functional consequences on GPCR-controlled cell migration and tissue localization. Neutrophils lacking all four GRK family members show increased chemotactic migration responses to a wide Combined depletion of GRK2 and GRK6 in T cells and B cells shows distinct functional outcomes for (a) one GPCR type in different cell types, and (b) different GPCRs in one cell type.

News < GPCRs in Oncology and Immunology Respiratory infections predominate after day 100 following B-cell maturation antigen-directed CAR T-cell therapy Published date September 26, 2023 Abstract "Infections are an important complication after B-cell maturation antigen (BCMA)-directed chimeric antigen receptor (CAR) T-cell therapy and risks may differ between the early and late periods. Respiratory infections predominate after BCMA CAR T-cell therapy, particularly after day 100.

< GPCR News < GPCRs in Oncology and Immunology GPR4 in the pH-dependent migration of melanoma cells in In this study, we investigated the pH-dependent migration of GPR4 wildtype/overexpressing SK-Mel-28 cells Migration of GPR4 overexpressing SK-Mel-28 cells was enhanced in a range of pH 6.5 - pH 7.5 as compared to controls in the impedance-based electrical wounding and migration assay. Results indicate that GPR4 is involved in the migration of melanoma cells, especially in the tumor periphery

Likewise, lysophosphatidic acid receptor 1 (LPA1) is implicated in cancer cell proliferation and migration LPA or alkyl-OMPT inhibited CXCL12-induced migration in various cancer cells that endogenously express Conversely, CXCL12-induced calcium signaling and migration were increased in LPAR1 knockout cells, and LPA1-selective antagonists enhanced CXCL12-induced Gαi/o signaling and cell migration in the parental Ultimately, complete inhibition of cell migration toward CXCL12 and alkyl-OMPT was only achieved in the

< GPCR News < GPCRs in Oncology and Immunology CCL5-producing migratory dendritic cells guide CCR5+ monocytes into the draining lymph nodes Published date March 21, 2023 Abstract "Dendritic cells (DCs) and monocytes capture, transport, and present antigen to cognate T cells in the draining lymph nodes (LNs) in a CCR7 observed that both CD88hiCD26lomonocytes and CD88-CD26hi cDCs captured inhaled antigens in the lung and migrated Authors Kavita Rawat , Anita Tewari , Xin Li , Arlind B Mara , William T King , Sophie L Gibbings , Chinaza

date April 4, 2024 Abstract "Efficient induction of humoral immune responses depends on orchestrated migration of B cells within lymphoid organs, which is governed by G protein-coupled receptors (GPCRs) responding recruits a specific GRK to chemoattractant receptors and plays an important role in the control of B cell migration during humoral immune responses. Authors Taiichiro Shirai , Akiko Nakai , Kazuhiro Suzuki Tags B cell migration , COMMD3/8 complex , GRK

migration Published date February 1, 2023 Abstract "C-X-C chemokine receptor 4 (CXCR4) is widely overexpressed Costimulation of CXCR4 and HRH1 also significantly enhances CXCL12-induced MDA-MB-231 cell migration, while histamine alone does not induce cell migration. Synergistic effects on calcium flux and cell migration are inhibited by the Gαi inhibitor pertussis toxin Enhanced calcium signaling and cell migration are also observed in NCI-H23 and HeLa cells, which coexpress

< GPCR News < GPCRs in Oncology and Immunology TIPE proteins control directed migration of human T cells Published date November 11, 2023 Abstract "Tissue infiltration by circulating leukocytes via directed migration of human CD4+ T cells. migration. phospholipid signaling pathways to control directed migration.

in Oncology and Immunology Autocrine proteinase-activated receptor signaling in PC3 prostate cancer cells PARs are highly expressed in many cancer cells, including prostate cancer (PCa), and regulate various In this study, we examined the androgen-independent human prostatic cancer cell line PC3 and find the We further examined PAR1 and PAR2 regulation of PCa cell proliferation and migration and found that absence of PAR1 promotes PC3 cell migration and suppresses cell proliferation, whereas PAR2 deficiency showed

and Immunology Dynamic Phosphoproteomics of BRS3 Activation Reveals the Hippo Signaling Pathway for Cell Migration Published date June 25, 2023 Abstract "Bombesin receptor subtype-3 (BRS3) is an orphan G-protein The lung cancer cell line H1299-BRS3 was treated with MK-5046, an agonist of BRS3, for different durations migration was further confirmed by kinase inhibition. Our data collectively demonstrate that BRS3 activation contributes to cell migration through downregulation

< GPCR News < GPCRs in Oncology and Immunology High expression of GPR50 promotes the proliferation, migration was analyzed in HCC patients (gene expression omnibus database (GEO) (GSE45436)) and detected in HCC cell 7919, and the results showed that GPR50 was significantly up-regulated in HCC patients and CBRH-7919 cell , migration, and autophagy of CBRH-7919. Taken together, GPR50 may promote HCC progression via CCT6A-induced proliferation and PGK1-induced migration

< GPCR News < GPCRs in Oncology and Immunology A2aR on lung adenocarcinoma cells: A novel target for Here, we identify that A2aR is specifically expressed on tumor cells from lung adenocarcinoma (LUAD) chemokines and polarizing factors through activating PI3K/AKT/NF-κB pathway, thereby promoting the migration Together, our studies demonstrate that A2aR on LUAD cells drives TAMs migration and polarization, and Tags A2aR , Lung adenocarcinoma , Migration , Polarization , Targeted therapy , Tumor-associated macrophages

Oncology and Immunology The β2-adrenergic receptor associates with CXCR4 multimers in human cancer cells established, there is still a lack of consensus regarding class A and B GPCR multimerization. PIE-FCCS can resolve membrane protein density, diffusion, and multimerization state in live cells at human lung cancer cells. than in COS-7 and CHO cells and in a ligand-dependent manner.

Immunology Systems Pharmacodynamic Model of Combination Gemcitabine and Trabectedin in Pancreatic Cancer Cells Part II: Cell Cycle, DNA Damage Response, and Apoptosis Pathways Published date October 31, 2023 Abstract , we reported the synergistic cytotoxic effects of gemcitabine and trabectedin on pancreatic cancer cells growth and migration, specifically the RTK-, integrin-, GPCR-, and calcium-signaling pathways. Here we describe drug effects on pathways associated with cell cycle, DNA damage response (DDR), and

GPCR News < GPCRs in Oncology and Immunology G protein coupled receptor transcripts in human immune cells We have used the method to profile GPCR transcripts in white blood cells (WBCs)-B, CD4, CD8, NK, and dendritic cells; monocytes, and macrophage-like monocytes treated with granulocyte-macrophage colony-stimulating factor-as well as platelets. On average, the white cells studied expressed 160 receptor mRNAs (range, 123-206).

Registration Logo Contest Committee Sponsors GPCR Retreat Program < Back to schedule Interaction with the cell adhesion molecule NEGR1 affects mGluR5 cell signalling Date & Time Friday, November 3rd / 1:30 PM Abstract Ribeiro has supervised eleven M.Sc. and six Ph.D. students, as well as five post-doctorate fellows. Nowadays, her research group comprises four undergraduates, two M.Sc., and six Ph.D. students, as well These drugs were shown to be very effective to rescue the cell death observed in a mouse model of Huntington

Published date January 2, 2023 Abstract “ Vasoactive intestinal peptide (VIP) receptor 2 (VIPR2) is a class B cell lines, promoted cell proliferation. proliferation in VIPR2-overexpressing MCF-7 and MDA-MB-231 cells. was greater than that in control cells, suggesting the increased PKA activity. at the same level as observed in EGFP-expressing cells treated with U0126.

We detected the expression of GPR37 in NSCLC tissues and cell lines. The study explored the influence of GPR37 on tumor cell proliferation. Furthermore, we examined the effects of GPR37 on tumor cell apoptosis and invasion. invasion, migration, and proliferation, suppresses cell apoptosis, heightens resistance to cisplatin Conversely, we observed that GPR37 knockdown suppresses NSCLC cell invasion, migration, and proliferation

Chemokines are chemotactic cytokines whose canonical functions govern movement of receptor expressing cells chemokines play paradoxical roles in both the directed emigration of metastatic, receptor-expressing cancer cells out of the tumor as well as immigration of tumor infiltrating immune cells that culminate in a tumor Authors Donovan Drouillard, Brian T Craig, Michael B Dwinell Tags Chemokine receptor; cell migration; Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call for GPCR

combination of brentuximab vedotin and chidamide synergistically suppresses the proliferation of T-cell lymphoma cells through the enhancement of apoptosis Published date November 3, 2023 Abstract " Purpose : Peripheral T-cell lymphoma (PTCL) is an aggressive disease with a poor prognosis. HH cells, originating from an aggressive cutaneous T-cell lymphoma, were used as an experimental model The combined effects of chidamide and BV were demonstrated in a study of HH-cell xenograft mice; mean

of 10 kDa (IP-10/CXCL10) is a dual-function CXC chemokine that coordinates chemotaxis of activated T cells and natural killer (NK) cells via interaction with its G protein-coupled receptor (GPCR), CXC chemokine migration, second messenger signaling downstream of CXCR3, and flow cytometry of CHO cells and primary human T lymphocytes and endothelial cells. loss of the four endmost C-terminal residues did not affect the inhibitory properties of CXCL10 on migration

Immunology Pharmacological inhibition of neuropeptide Y receptors Y1 and Y5 reduces hypoxic breast cancer migration hypoxia inducible factors, which sensitizes these receptors to NPY stimulation leading to enhanced migration Methods/results: Here, we measured the effects of NPY1R and NPY5R antagonists in normoxia and hypoxia on migration proliferation, cell migration and invasion, and spheroid growth and invasion. There were some discrepancies in the responses of each cell line to the isoform-specific antagonists

Oncology and Immunology CCR7 acts as both a sensor and a sink for CCL19 to coordinate collective leukocyte migration Published date September 1, 2023 Abstract "Immune responses rely on the rapid and coordinated migration Whereas it is well established that single-cell migration is often guided by gradients of chemokines receptor (GPCR) CCR7, we demonstrate that in addition to its role as the sensory receptor that steers migration This mechanism drives complex collective migration patterns, enabling DCs to create or sharpen chemotactic

Immunology Comparative Analysis of the GNAI Family Genes in Glioblastoma through Transcriptomics and Single-Cell regulation of actin cytoskeleton organization by the kinase effectors of Rho GTPases" and "Immune response B cell antigen receptor (BCR) pathway". A single-cell analysis was used to assess GNAI3 expression in GBM. Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call for GPCR

progression in MCF-7 cells Published date March 1, 2024 Abstract "Vasoactive intestinal peptide (VIP In this study, we examined the role of VIPR2 in cell cycle progression. in MCF-7 cells. The percentage of cells in the S-M phase was decreased in MCF-7 cells treated with KS-133. In MCF-7 cells stably-expressing VIPR2, KS-133 decreased PI3K activity and cAMP levels.

< GPCR News < GPCRs in Oncology and Immunology Activation of orphan receptor GPR132 induces cell differentiation Here, we showed that genetic activation of the orphan GPCR GPR132 significantly induced cell differentiation Notably, GPR132 activation by 8GL promoted differentiation and reduced colony formation in human AML cell We further showed that the combination of 8GL and an mTOR inhibitor synergistically elicited AML cell impaired tumor growth and extended mouse survival in an AML xenograft model accompanied by enhanced cell

News < GPCRs in Oncology and Immunology The orphan G protein-coupled receptor 141 expressed in myeloid cells High GPR141 messenger RNA levels were expressed in myeloid-lineage cells, including neutrophils (CD11b Gpr141 -/- mice, which we independently generated, displayed almost no abnormalities in myeloid cell myelin oligodendrocyte glycoprotein 35-55-specific T cells. , experimental autoimmune encephalomyelitis , monocytes , myeloid cells .

< GPCR News < GPCRs in Oncology and Immunology The EBI2 receptor is coexpressed with CCR5 in CD4+ T cells Methods: We identified GPCRs expressed in primary CD4+CCR5+ T cells by multi-RT-qPCR. Cell lines expressing EBI2 were established by transduction with HIV vectors. The amount of HIV reverse transcripts was similar in cells expressing or not EBI2. Conclusions: EBI2 expression in CD4+CCR5+ cells boosts HIV-1 R5 productive infection.