< GPCR News < GPCRs in Oncology and Immunology GPR4 in the pH-dependent migration of melanoma cells in In this study, we investigated the pH-dependent migration of GPR4 wildtype/overexpressing SK-Mel-28 cells Migration of GPR4 overexpressing SK-Mel-28 cells was enhanced in a range of pH 6.5 - pH 7.5 as compared to controls in the impedance-based electrical wounding and migration assay. In Boyden chamber experiments, GPR4 overexpression only increased migration at pH 7.5 in a Matrigel-free

Oncology and Immunology CCR7 acts as both a sensor and a sink for CCL19 to coordinate collective leukocyte migration Published date September 1, 2023 Abstract "Immune responses rely on the rapid and coordinated migration Whereas it is well established that single-cell migration is often guided by gradients of chemokines receptor (GPCR) CCR7, we demonstrate that in addition to its role as the sensory receptor that steers migration This mechanism drives complex collective migration patterns, enabling DCs to create or sharpen chemotactic

< GPCR News < GPCRs in Oncology and Immunology TIPE proteins control directed migration of human T cells Published date November 11, 2023 Abstract "Tissue infiltration by circulating leukocytes via directed migration like (TIPE or TNFAIP8L) family of proteins are newly described pilot proteins that control directed migration demonstrated here that two human TIPE family members, TNFAIP8 and TIPE2 were essential for directed migration paradigm for how human T cells integrate GPCR and phospholipid signaling pathways to control directed migration

Oncology and Immunology LPA1-mediated inhibition of CXCR4 attenuates CXCL12-induced signaling and cell migration Likewise, lysophosphatidic acid receptor 1 (LPA1) is implicated in cancer cell proliferation and migration LPA or alkyl-OMPT inhibited CXCL12-induced migration in various cancer cells that endogenously express Conversely, CXCL12-induced calcium signaling and migration were increased in LPAR1 knockout cells, and LPA1-selective antagonists enhanced CXCL12-induced Gαi/o signaling and cell migration in the parental

< GPCR News < GPCRs in Oncology and Immunology Leukotriene B4 receptor 2 governs macrophage migration Transcriptomic analysis of Ltb4r2-/- and WT macrophages suggested a role for BLT2 in macrophage migration morpholino-mediated knockdown of blt2a or chemical inhibition of BLT2 signaling impairs macrophage migration applicability of our zebrafish findings to mammals by showing that macrophages of Ltb4r2-/- mice have defective migration Collectively, our results demonstrate that BLT2 mediates macrophage migration during inflammation, which

activation of CXC chemokine receptor 4 and histamine receptor H1 enhances calcium signaling and cancer cell migration Costimulation of CXCR4 and HRH1 also significantly enhances CXCL12-induced MDA-MB-231 cell migration, while histamine alone does not induce cell migration. Synergistic effects on calcium flux and cell migration are inhibited by the Gαi inhibitor pertussis toxin Enhanced calcium signaling and cell migration are also observed in NCI-H23 and HeLa cells, which coexpress

Immunology Pharmacological inhibition of neuropeptide Y receptors Y1 and Y5 reduces hypoxic breast cancer migration hypoxia inducible factors, which sensitizes these receptors to NPY stimulation leading to enhanced migration Methods/results: Here, we measured the effects of NPY1R and NPY5R antagonists in normoxia and hypoxia on migration NPY5R in hypoxia compared to normoxia more greatly reduced MAPK signaling, cell proliferation, cell migration

< GPCR News < GPCRs in Oncology and Immunology High expression of GPR50 promotes the proliferation, migration cDNA was transfected into HCC cell line CBRH-7919, and we found that Gpr50 promoted the proliferation, migration Taken together, GPR50 may promote HCC progression via CCT6A-induced proliferation and PGK1-induced migration

date April 4, 2024 Abstract "Efficient induction of humoral immune responses depends on orchestrated migration recruits a specific GRK to chemoattractant receptors and plays an important role in the control of B cell migration Authors Taiichiro Shirai , Akiko Nakai , Kazuhiro Suzuki Tags B cell migration , COMMD3/8 complex , GRK

Immunology Dynamic Phosphoproteomics of BRS3 Activation Reveals the Hippo Signaling Pathway for Cell Migration dephosphorylation and nucleus localization of the Yes-associated protein (YAP), and its association with cell migration Our data collectively demonstrate that BRS3 activation contributes to cell migration through downregulation

chemokines and polarizing factors through activating PI3K/AKT/NF-κB pathway, thereby promoting the migration Together, our studies demonstrate that A2aR on LUAD cells drives TAMs migration and polarization, and Tags A2aR , Lung adenocarcinoma , Migration , Polarization , Targeted therapy , Tumor-associated macrophages

and GPR15L in blood and synovial tissue samples from RA patients, as well as to perform a functional migration Migration assays were performed using PBMCs isolated from these individuals in response to the synthetic GPR15 and GPR15L are present in the synovial tissue of RA patients and GPR15L promotes migration of PBMCs

atypical receptor ACKR3 both respond to CXCL12 but induce different effector responses to regulate cell migration While CXCR4 couples to G proteins and directly promotes cell migration, ACKR3 is G protein-independent regulate extracellular chemokine levels and maintain CXCR4 responsiveness, thereby indirectly influencing migration

constitutes GPCR-mediated phospholipase C (PLC) signaling and is essential for neutrophil polarization and migration activation; impaired dynamics of actin polymerization; and consequently, defects in cell polarization and migration

We further examined PAR1 and PAR2 regulation of PCa cell proliferation and migration and found that absence of PAR1 promotes PC3 cell migration and suppresses cell proliferation, whereas PAR2 deficiency showed

a lipid that activates G protein-coupled receptors (GPCRs), enhancing proliferation, adhesion, and migration In some model systems, CCN1 and CCN2 play key roles in LPA/S1P-induced cell migration and proliferation

unfavorable prognosis. (2) It was demonstrated that GPR37 positively regulates NSCLC cell invasion, migration Conversely, we observed that GPR37 knockdown suppresses NSCLC cell invasion, migration, and proliferation

and dendritic cells) and systematically addressed the functional consequences on GPCR-controlled cell migration Neutrophils lacking all four GRK family members show increased chemotactic migration responses to a wide GPCR ligands, whereas combinatorial GRK depletions in other immune cell types lead to pro- and anti-migratory

1-77) using surface plasmon resonance for glycosaminoglycan (GAG) binding affinity, assays for cell migration loss of the four endmost C-terminal residues did not affect the inhibitory properties of CXCL10 on migration

requirements for GPCR activation of signaling pathways related to cell survival, proliferation, and migration

Authors Donovan Drouillard, Brian T Craig, Michael B Dwinell Tags Chemokine receptor; cell migration;

Upregulation of CXCR2 is closely associated with the migration of neutrophils and monocytes.

receptor-ligand interactions are highly relevant for its different physiological effects, e. g. the migration

< GPCR News < GPCRs in Oncology and Immunology CCL5-producing migratory dendritic cells guide CCR5+ monocytes Since only migratory DCs express this chemokine receptor, it is unclear how monocytes reach the LN. Migratory cDCs, however, upregulated Ccr7, Ccl17, Ccl22, and Ccl5. Migratory monocytes expressed Ccr5, a high-affinity receptor for Ccl5. observed that both CD88hiCD26lomonocytes and CD88-CD26hi cDCs captured inhaled antigens in the lung and migrated

In addition, AJ-3 inhibited the migration of LNCaP prostate cancer cells in wound healing assays.

also associated with a significant disruption in their cellular metabolism and a decreased ability to migrate

modification that supports membrane interactions of proteins involved in various cellular processes, including migration

Results show that the elevated expression of UCA1 enhances cell proliferation, invasive migration, and