Recent advancements in transcriptomic profiling have provided new insights into the expression patterns of GPCRs across different cell types. The study reports that human white blood cells express an average of 160 GPCR mRNAs, ranging from 123 semi-quantitative multiplex PCR method, which allowed for the detection of both abundant and rare GPCR transcripts , including B cells, CD4+ T cells, CD8+ T cells, natural killer (NK) cells, and dendritic cells.

Using multiplexed single-cell transcriptomics, we analyze human neural precursor cells (hNPCs) from sporadic

into studies focused on obtaining detailed molecular information in the intracellular context ("in-cell ") or those focused on characterizing molecules or events at the cell surface ("on-cell"). In this review, we outline some key NMR techniques applied for on-cell NMR studies through both solution with cell surface membrane proteins such as G-protein coupled receptors (GPCRs) and receptor tyrosine We also provide a brief survey of the applicability of on-cell NMR approaches to other classes of cell

the human A2A adenosine receptor (A2AAR), a class A G protein-coupled receptor (GPCR) for 19F-NMR and single-molecule

One suggested theory is that cannabinoids regulate a variety of physiological processes within the cells Murine L6 skeletal muscle cells were used in order to clarify additional possible molecular signaling Skeletal muscle cells have often been used; it is well-documented that they express cannabinoid receptors In the current work, it is demonstrated that skeletal muscle cells exhibit functional expression of CB1 Thus, in skeletal muscle cells, a possible physiological expression of CB1 receptors was identified.

August 2022 Deciphering the signaling mechanisms of β-arrestin1 and β-arrestin2 in regulation of cancer cell They have participated in various cellular processes such as cell proliferation, migration, apoptosis , and transcription via canonical and noncanonical pathways.

Analysis of CD4+ T cells activated under T helper (Th) 1, Th2, Th17 and regulatory T (Treg) cell differentiation Through multimodal single-cell CITE- and TCR-sequencing, we detected GLP-1R expression in 29–34% of the FoxP3+CD25+CD127- iTreg cells. GLP-1R in human CD4+ T cells. Given the high induction of GLP-1R in human iTreg cells, we hypothesize that GLP-1R+ iTreg cells play

August 2022 TAS2R supports odontoblastic differentiation of human dental pulp stem cells in the inflammatory Background: Inflammatory microenvironment promotes odontoblastic differentiation in human dental pulp stem cells

Canonical chemokine receptors – scavenging “decoys” Chemokine receptors coordinate cell migration upon class of heterotrimeric G proteins, which, in turn, activate signaling pathways that ultimately lead to cell CCR2 is an example of a dual-function receptor that directly regulates both cell migration and scavenging and almost completely lost in the GRK2/3/5/6 KO cells, whereas constitutive receptor internalization Scavenging may allow cells to continuously migrate by remaining responsive to chemokines (S.

August 2022 Luciferase-based GloSensor™ cAMP assay: Temperature optimization and application to cell-based Monitoring intracellular cAMP levels can help identify orthosteric agonists and antagonists, as well As well, it has not been reported whether the effects of temperature on biosensor activity are reversible We also present a detailed protocol for monitoring cAMP levels in live cells expressing endogenous or Temperature optimization studies were carried out using HEK293H cells transiently transfected with the

October 2022 "Study of small molecule binding to live cells provides important information on the characterization Here we developed and validated a label-free, liquid chromatography-mass spectrometry (LC-MS) based cell This versatile method allows quantitative characterization of ligand binding to cell surface expressed

September 2022 β2-Adrenergic Receptor Expression and Intracellular Signaling in B Cells Are Highly Dynamic In the past, treatment of arthritic B cells with a β2-adrenergic receptor (β2-ADR) agonist has been shown In this study, the expression and signaling of β2-ADR in B cells during collagen-induced arthritis (CIA ) were investigated to provide an explanation of why only B cells from arthritic mice are able to improve Splenic B cells were isolated via magnetic-activated cell sorting (MACS).

October 2022 "Single-molecule counting techniques enable a precise determination of the intracellular oligomerization in receptor signaling came from ensemble biochemical and biophysical assays, innovations in single-molecule Here, we review recent developments in single-molecule counting with a focus on photobleaching step counting and the emerging technique of quantitative single-molecule localization microscopy-with a particular

September 2022 Cell-Type-Specific Effects of the Ovarian Cancer G-Protein Coupled Receptor (OGR1) on However, in epithelial cells, OGR1 promotes epithelial to mesenchymal transition (EMT) and inflammation localization and alternative signaling pathways may be responsible for the differential effect of OGR1 in each cell

Direct Selection of DNA-Encoded Libraries for Biased Agonists of GPCRs on Live Cells. Bioorthogonal Tethering Enhances Drug Fragment Affinity for G Protein-Coupled Receptors in Live Cells Single-cell transcriptome analysis of NEUROG3+ cells during pancreatic endocrine differentiation with Single cell G-protein coupled receptor profiling of Transcription factor 21 expressing activated kidney FREE Symposium - IPI Surfacing (June 15, 2023) Training School on “Cell-based assays to study Adhesion

October 2022 Cholesterol-Dependent Dynamics of the Serotonin1A Receptor Utilizing Single Particle Tracking : Analysis of Diffusion Modes "G protein-coupled receptors (GPCRs) are signaling hubs in cell membranes To explore the role of cholesterol in lateral dynamics of GPCRs, we utilized single particle tracking Notably, in cholesterol-depleted cells, we observed an increase in the confined population of the receptor

therapeutically exploit GPCRs (Physiology’s ‘Swiss Army Knife’) vast repertoire of ways to control cell in Molecular Pharmacology - The Hauser Group Postdoctoral Scholar – iPSC in cardiac and endothelial cell by sphingosine kinase-1 GPR160 regulates the self-renewal and pluripotency of mouse embryonic stem cells identifies small molecules that inhibit midgut contractions GPCRs in Cardiology, Endocrinology, and Taste Single-cell transcriptomic atlas of enteroendocrine cells along the murine gastrointestinal tract Intracellular

September 2022 Cell Surface Calcium-Sensing Receptor Heterodimers: Mutant Gene Dosage Affects Ca 2+ Sensing chimeras subject to GABAB -dependent endoplasmic reticulum sorting to traffic mutant heterodimers to the cell Transfected HEK-293 cells were assessed for Ca2+o -stimulated Ca2+i mobilization using mutations in either In contrast, a single WT HH bundle was insufficient for maximal signaling and there was no functional

September 2022 "G protein-coupled receptors (GPCRs) are signaling hubs in cell membranes that regulate To explore the role of cholesterol in lateral dynamics of GPCRs, we utilized single particle tracking Notably, in cholesterol-depleted cells, we observed an increase in the confined population of the receptor

September 2022 GASP1 enhances malignant phenotypes of breast cancer cells and decreases their response , such as inhibition of cell proliferation, colony formation, migration, invasion and xenograft tumor growth in nude mice as well as induction of G1-phase cell cycle arrest, and vice versa. Besides, we found that GASP1 knockout obviously improved the response of breast cancer cells to paclitaxel Collectively, this study demonstrates that GASP1 enhances malignant behaviors of breast cancer cells

At the cellular level, the plasmatic membrane is a thin layer of lipids that surrounds the entire cell interplay between GPCRs and β-arrestins and how this complex operates on the plasma membrane of living cells Based on an ingenious combination of multicolor single-molecule microscopy approach with molecular dynamics dissected the sequence of events in receptor-β-arrestin interactions at the plasma membrane of living cells Cell, 185(24), 4560–4573.e19. https://doi.org/10.1016/j.cell.2022.10.018 Latorraca, N.R., Wang, J.K.,

November 2022 Deciphering the signaling mechanisms of β-arrestin1 and β-arrestin2 in regulation of cancer cell They have participated in various cellular processes such as cell proliferation, migration, apoptosis , and transcription via canonical and noncanonical pathways.

September 2022 Lack of Oestrogen Receptor Expression in Breast Cancer Cells Does Not Correlate with Kisspeptin In KISS1R overexpressing triple-negative breast cancer (TNBC) cells, stimulation has been associated MDA-MB-231 cells are metastatic when implanted in mice while BT-20 are not and remain epithelial-like However, KP-10 stimulation induced migration of MDA-MB-231, but not BT-20 cells, in a calcium-dependent Interestingly, both cell lines displayed different complements of β-arrestin 1 and 2 expression.

August 2022 Production of human A 2A AR in lipid nanodiscs for 19 F-NMR and single-molecule fluorescence the human A2A adenosine receptor (A2AAR), a class A G protein-coupled receptor (GPCR) for 19F-NMR and single-molecule

histamine receptor 2 (H2R), a G protein-coupled receptor (GPCR), which is expressed in a wide variety of cell A growing number of GPCRs have been shown to be localized in the nucleus and contribute toward transcriptional study, for the first time, we demonstrate the nuclear localization of H2R in lymphatic endothelial cells , such as the functional relevance of this localization, and whether H2R can contribute directly to transcriptional

August 2022 GPR3 expression in retinal ganglion cells contributes to neuron survival and accelerates GPR3 was relatively highly expressed in retinal ganglion cells (RGCs).

mammary gland (MG) undergoes remodeling, which involves alveolar differentiation of mammary epithelial cells and gene and protein expression of major milk protein and lipid synthesizing markers. using the HC11 cells hypothesized that THC and CBD will negatively impact the synthesis of milk proteins and lipids, as well as lipid markers in HC11 cells. In conclusion, 10μM THC and CBD altered the differentiation of HC11 cells, in part via the CB2 receptor

September 2022 Opposite Effects of Src Family Kinases on YAP and ERK Activation in Pancreatic Cancer Cells IGF-1 receptors and G protein-coupled (GPCR) signaling systems leading to mitogenic signaling in PDAC cells agonist neurotensin induced rapid activation of Src family of tyrosine kinases (SFKs) within PANC-1 cells by FAK phosphorylation at Tyr576/577 and Tyr861, sensitive biomarkers of SFK activity within intact cells and suppessed the growth of MiaPaCa-2 cells xenografted into the flank of nude mice.