NKTCL), a representative disease model to study EBV-induced lymphomagenesis, incorporating genomic, transcriptomic Single-cell transcriptome further delineated the tumor microenvironment as immune-inflamed, -deficient cell killing in NKTCL organoid. Sai-Juan Chen , Wei-Li Zhao Tags CCR1 , Epstein-Barr virus , Lymphoma , Microenvironment , Multi-omics , Single-cell transcriptome Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News

GPCRs in Oncology and Immunology Comparative Analysis of the GNAI Family Genes in Glioblastoma through Transcriptomics and Single-Cell Technologies Published date October 23, 2023 Abstract Glioblastoma multiforme (GBM) conducted to explore the role of GNAI3 in co-expressed genes and associated signaling pathways using a transcript regulation of actin cytoskeleton organization by the kinase effectors of Rho GTPases" and "Immune response B cell A single-cell analysis was used to assess GNAI3 expression in GBM.

< GPCR News < GPCRs in Oncology and Immunology Single cell G-protein coupled receptor profiling of Transcription Experimental approach: RNA sequencing and single cell qRT-PCR were performed on mouse kidneys after unilateral Key results: Transcription factor 21 (Tcf21)+ cells that expressed 2 or 3 of Postn, Acta2 and Pdgfra Tcf21+ α-smooth muscle actin (α-SMA)+ interstitial cells accumulated in the kidneys of mice with UUO Fifty-six GPCRs were upregulated in single Tcf21+ kidney fibroblasts, the most upregulated being Adgra2

cells and platelets Published date September 27, 2024 Abstract "G-protein coupled receptors (GPCRs) We have used the method to profile GPCR transcripts in white blood cells (WBCs)-B, CD4, CD8, NK, and dendritic cells; monocytes, and macrophage-like monocytes treated with granulocyte-macrophage colony-stimulating factor-as well as platelets. On average, the white cells studied expressed 160 receptor mRNAs (range, 123-206).

To address this issue, we generated mouse models of single, combinatorial and complete GRK knockouts in four primary immune cell types (neutrophils, T cells, B cells and dendritic cells) and systematically Combined depletion of GRK2 and GRK6 in T cells and B cells shows distinct functional outcomes for (a) one GPCR type in different cell types, and (b) different GPCRs in one cell type. ; GRK; T cells; dendritic cells; immune cell trafficking; leukocytes; neutrophils.

CCK8 and cell cloning were conducted to determine the proliferation ability of H1299 and Anip973 cells Furthermore, Transcriptome sequencing was performed to show enrichment genes and pathways. Further, RGS20 accelerated cell proliferation by increasing autophagy. Transcriptomic sequencing suggested the involvement of the Hippo signaling pathway in the action of RGS20 in RGS20 knock-down cells.

< GPCR News < GPCRs in Oncology and Immunology The EBI2 receptor is coexpressed with CCR5 in CD4+ T cells Methods: We identified GPCRs expressed in primary CD4+CCR5+ T cells by multi-RT-qPCR. Cell lines expressing EBI2 were established by transduction with HIV vectors. The amount of HIV reverse transcripts was similar in cells expressing or not EBI2. Conclusions: EBI2 expression in CD4+CCR5+ cells boosts HIV-1 R5 productive infection.

Registration Logo Contest Committee Sponsors GPCR Retreat Program < Back to schedule Interaction with the cell adhesion molecule NEGR1 affects mGluR5 cell signalling Date & Time Friday, November 3rd / 1:30 PM Abstract Ribeiro has supervised eleven M.Sc. and six Ph.D. students, as well as five post-doctorate fellows. Nowadays, her research group comprises four undergraduates, two M.Sc., and six Ph.D. students, as well These drugs were shown to be very effective to rescue the cell death observed in a mouse model of Huntington

Full Agenda Student Flash Presentations Health and Disease, Metabolism, Nervous System, Proteomics and Transcriptomics To Their Niche And Leads To Myeloid Skewing Emmanouil Kyrloglou A single cell GPCR map of thermogenic Emmanouil Kyrloglou on the web Adhesion GPCR Consortium LinkedIn A single cell GPCR map of thermogenic These data demonstrate that Adgrf5 transcript variants are cell-specific in the kidney. We performed single-nucleus RNA sequencing on male and female kidneys. snRNAseq revealed abundant, cell-specific

Abstract "We explored the dysregulation of G-protein-coupled receptor (GPCR) ligand systems in cancer transcriptomics receptors, are the targets of multiple drugs displaying anti-growth effects in large-scale, cancer cell , Guanming Wu, Gioacchino Natoli, J Silvio Gutkind , Francesco Raimondi Tags GPCR , cancer , cancer cell lines , cell-cell communication , drug repurposing , personalized medicine , signaling network , survival analysis , transcriptomics .

< GPCR News < GPCRs in Oncology and Immunology miR-19a may function as a biomarker of oral squamous cell Using a transcriptomic analysis of over 37,000 nuclei, we identified twelve distinct clusters of cells corresponding to temperature-sensing arista neurons, humidity-sensing sacculus neurons, and support cells We found that each cell type could be characterized by a unique expression profile of ion channels, GPCR signaling molecules, synaptic vesicle cycle proteins, and cell adhesion molecules.

News < GPCRs in Oncology and Immunology Distinct Activation Mechanisms of CXCR4 and ACKR3 Revealed by Single-Molecule atypical receptor ACKR3 both respond to CXCL12 but induce different effector responses to regulate cell While CXCR4 couples to G proteins and directly promotes cell migration, ACKR3 is G protein-independent Multiple active-like ACKR3 conformations are populated in response to agonists, compared to the single Much of the conformational heterogeneity of ACKR3 is linked to a single residue that differs between

Oncology and Immunology The β2-adrenergic receptor associates with CXCR4 multimers in human cancer cells PIE-FCCS can resolve membrane protein density, diffusion, and multimerization state in live cells at human lung cancer cells. than in COS-7 and CHO cells and in a ligand-dependent manner. Authors Junyi Liang , Mohamed Seghiri , Pradeep Kumar Singh , Hyeon Gyu Seo , Ji Yeong Lee , Yoonjung

combination of brentuximab vedotin and chidamide synergistically suppresses the proliferation of T-cell lymphoma cells through the enhancement of apoptosis Published date November 3, 2023 Abstract " Purpose : Peripheral T-cell lymphoma (PTCL) is an aggressive disease with a poor prognosis. HH cells, originating from an aggressive cutaneous T-cell lymphoma, were used as an experimental model The combined effects of chidamide and BV were demonstrated in a study of HH-cell xenograft mice; mean

< GPCR News < GPCRs in Oncology and Immunology CCL5-producing migratory dendritic cells guide CCR5+ monocytes into the draining lymph nodes Published date March 21, 2023 Abstract "Dendritic cells (DCs) and monocytes capture, transport, and present antigen to cognate T cells in the draining lymph nodes (LNs) in a CCR7 type 2 conventional (c)DCs, despite the downregulation of Xcr1, Clec9a, H2-Ab1, Sirpa, and Clec10a transcripts Jakubzick Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

G protein signaling protein 6 alleviates acute lung injury by inhibiting inflammation and promoting cell Organoid culture was used to assess the stemness and self-renewal capacity of alveolar epithelial type II cells In addition, RGS6 overexpression decreased ROS production as well as proinflammatory factor transcription in macrophages and decreased apoptosis in epithelial cells. a protective role in ALI not only in early inflammatory responses but also in endogenous lung stem cell

progression in MCF-7 cells Published date March 1, 2024 Abstract "Vasoactive intestinal peptide (VIP In this study, we examined the role of VIPR2 in cell cycle progression. in MCF-7 cells. The percentage of cells in the S-M phase was decreased in MCF-7 cells treated with KS-133. In MCF-7 cells stably-expressing VIPR2, KS-133 decreased PI3K activity and cAMP levels.

News < GPCRs in Oncology and Immunology Respiratory infections predominate after day 100 following B-cell maturation antigen-directed CAR T-cell therapy Published date September 26, 2023 Abstract "Infections are an important complication after B-cell maturation antigen (BCMA)-directed chimeric antigen receptor (CAR) T-cell therapy and risks may differ between the early and late periods. Respiratory infections predominate after BCMA CAR T-cell therapy, particularly after day 100.

in Oncology and Immunology Autocrine proteinase-activated receptor signaling in PC3 prostate cancer cells PARs are highly expressed in many cancer cells, including prostate cancer (PCa), and regulate various In this study, we examined the androgen-independent human prostatic cancer cell line PC3 and find the Using genetically encoded PAR cleavage biosensors, we showed that PC3 cells secrete proteolytic enzymes of PAR1 promotes PC3 cell migration and suppresses cell proliferation, whereas PAR2 deficiency showed

Immunology Systems Pharmacodynamic Model of Combination Gemcitabine and Trabectedin in Pancreatic Cancer Cells Part II: Cell Cycle, DNA Damage Response, and Apoptosis Pathways Published date October 31, 2023 Abstract , we reported the synergistic cytotoxic effects of gemcitabine and trabectedin on pancreatic cancer cells Here we describe drug effects on pathways associated with cell cycle, DNA damage response (DDR), and The SPD model was subsequently incorporated into our previously-established cell cycle model, forming

expression of GPR50 promotes the proliferation, migration and autophagy of hepatocellular carcinoma cells was analyzed in HCC patients (gene expression omnibus database (GEO) (GSE45436)) and detected in HCC cell 7919, and the results showed that GPR50 was significantly up-regulated in HCC patients and CBRH-7919 cell Gpr50 cDNA was transfected into HCC cell line CBRH-7919, and we found that Gpr50 promoted the proliferation Authors Weiming Zhao , Lingling Xi , Guoying Yu , Gaiping Wang , Cuifang Chang Source Contribute to the

protein-coupled receptors (GPCRs), enhancing proliferation, adhesion, and migration in many types of cancer cells Our group previously reported that LPA induces production of CCN1 protein in human prostate cancer cell In these cells, the mitogenic activity of LPA is mediated by LPA Receptor 1 (LPAR1), a GPCR. for LPA/S1P-induced CCN1/2 typically involve activation of the small GTP-binding protein Rho and the transcription In some model systems, CCN1 and CCN2 play key roles in LPA/S1P-induced cell migration and proliferation

in Oncology and Immunology LPA1-mediated inhibition of CXCR4 attenuates CXCL12-induced signaling and cell Results: We observed that CXCR4 forms heteromers with LPA1 in recombinant HEK293A cells and the human breast cancer cell line MDA-MB-231. MDA-MB-231 cells but not in LPA1-deficient cells. have been evidenced across various cell lines.

< GPCR News < GPCRs in Oncology and Immunology GPR4 in the pH-dependent migration of melanoma cells in pH-gradient) is a well-known driver of tumor progression and metastasis. In this study, we investigated the pH-dependent migration of GPR4 wildtype/overexpressing SK-Mel-28 cells Migration of GPR4 overexpressing SK-Mel-28 cells was enhanced in a range of pH 6.5 - pH 7.5 as compared Results indicate that GPR4 is involved in the migration of melanoma cells, especially in the tumor periphery

< GPCR News < GPCRs in Oncology and Immunology Activation of orphan receptor GPR132 induces cell differentiation Here, we showed that genetic activation of the orphan GPCR GPR132 significantly induced cell differentiation Notably, GPR132 activation by 8GL promoted differentiation and reduced colony formation in human AML cell We further showed that the combination of 8GL and an mTOR inhibitor synergistically elicited AML cell impaired tumor growth and extended mouse survival in an AML xenograft model accompanied by enhanced cell

Experimental design: We performed targeted sequencing, high-throughput drug screening, and single-cell genomic profiling on leukemia cell samples derived from patients with AML. understand the co-occurring mutation patterns and further investigated the mutation profiles in the single cells. The application of single-cell genomic sequencing unveiled the co-occurrence of variants at the individual

News < GPCRs in Oncology and Immunology The orphan G protein-coupled receptor 141 expressed in myeloid cells High GPR141 messenger RNA levels were expressed in myeloid-lineage cells, including neutrophils (CD11b Gpr141 -/- mice, which we independently generated, displayed almost no abnormalities in myeloid cell myelin oligodendrocyte glycoprotein 35-55-specific T cells. , experimental autoimmune encephalomyelitis , monocytes , myeloid cells .

in Oncology and Immunology Vasoactive intestinal peptide receptor 2 signaling promotes breast cancer cell cell lines, promoted cell proliferation. proliferation in VIPR2-overexpressing MCF-7 and MDA-MB-231 cells. was greater than that in control cells, suggesting the increased PKA activity. at the same level as observed in EGFP-expressing cells treated with U0126.