, referred to as "SynTAMs" for synaptic targeting molecules, that enable localized perturbations of cAMP We show that locally restricted suppression of postsynaptic cAMP levels or of cAMP-dependent protein-kinase Retrograde trans-synaptic rabies virus tracing revealed that postsynaptic cAMP signaling is required Given that postsynaptic latrophilin adhesion-GPCRs drive synapse formation and produce cAMP, we suggest that spatially restricted postsynaptic cAMP signals organize assembly of postsynaptic specializations

A role for BET proteins in regulating basal, dopamine-induced and cAMP/PKA-dependent transcription in The D1 receptor (D1R) is a Gαs/olf-coupled GPCR which activates a cAMP/PKA/DARPP-32 signalling cascade is unknown whether BET proteins, or Brd4 specifically, are involved in transcriptional activation by cAMP We further found that cAMP/PKA signalling promotes Brd4 recruitment to dopamine-induced genes in striatal

August 2022 Luciferase-based GloSensor™ cAMP assay: Temperature optimization and application to cell-based The second messenger cyclic adenosine monophosphate (cAMP) is a key mediator in many GPCR signaling pathways Monitoring intracellular cAMP levels can help identify orthosteric agonists and antagonists, as well The GloSensor™ cAMP assay enables real-time monitoring of signaling downstream of many GPCRs. GloSensor™ version is optimal for measuring cytosolic cAMP.

Overexpressed VIPR2 caused increases in the levels of cAMP and phosphorylated extracellular signal-regulated Additionally, phosphorylation of vasodilator-stimulated phosphoprotein (Ser157) and cAMP response element Together, these findings suggest that VIPR2 controls breast tumor growth by regulating the cAMP/PKA/ERK Tanimoto , Hitoshi Hashimoto , Yukio Ago Tags Breast cancer , Cell proliferation , ERK , GPCR , VIPR2 , cAMP

GPR30 constitutively and PDZ-dependently inhibits β1AR-mediated cAMP production. Furthermore, expression of GPR30 in MCF7 cells constitutively and PDZ-dependently inhibits β1AR-mediated cAMP AKAP5 also inhibits β1AR-mediated cAMP production, which is not additive with GPR30-promoted inhibition

studied hedgehog pathway repression by the GPCR GPR161, and the ankyrin repeat protein ANKMY2 that direct cAMP tested the role of cilia-generated signaling using a Gpr161 ciliary localization knock-in mutant that is cAMP The cAMP signaling-competent non-ciliary Gpr161 knock-in recapitulated Gpr161 loss-of-function tissue