inhibitor of GPR68 named Ogremorphin (OGM) to induce the iron mediated cell death pathway: ferroptosis Cell survival assays, via nuclei counting and cell titer glo, were used to demonstrate sensitivity to To determine GPR68 inhibition's mechanism of cell death we use DAVID pathway analysis of RNAseq. death in all thirteen glioblastoma cell lines tested, irrespective of genetic and phenotypic heterogeneity death.

receptor proteins are proficient in sensing external signals and initiating downstream pathways to control cell GPR56, a G-protein-coupled receptor, can be activated by its agonist to suppress ferroptosis-a form of cell death-and effectively mitigate ferroptosis-associated liver damage." , Boyi Gan Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

Registration Logo Contest Committee Sponsors GPCR Retreat Program < Back to schedule Interaction with the cell adhesion molecule NEGR1 affects mGluR5 cell signalling Date & Time Friday, November 3rd / 1:30 PM Abstract Ribeiro has supervised eleven M.Sc. and six Ph.D. students, as well as five post-doctorate fellows. Nowadays, her research group comprises four undergraduates, two M.Sc., and six Ph.D. students, as well These drugs were shown to be very effective to rescue the cell death observed in a mouse model of Huntington

histone deacetylase (HDAC) activities, protein modifications, signaling pathways, and gene expression in cells within the tumor microenvironment, particularly in tumor and immune cells. Shan Li, Yixin Duan, Shudi Luo, Fangxin Zhou, Qingang Wu, Zhimin Lu Tags acetate , butyrate , cancer , cell death , immune response , metabolism , proliferation , propionate , short chain fatty acid Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News GPCR Jobs Call for GPCR papers

News < GPCRs in Oncology and Immunology Respiratory infections predominate after day 100 following B-cell maturation antigen-directed CAR T-cell therapy Published date September 26, 2023 Abstract "Infections (CAR) T-cell therapy and risks may differ between the early and late periods. functions were calculated based on time to first respiratory infection using dates of infection-free death Respiratory infections predominate after BCMA CAR T-cell therapy, particularly after day 100.

< GPCR News < GPCRs in Oncology and Immunology NPFF stimulates human ovarian cancer cell invasion by Epithelial ovarian cancer (EOC) is a leading cause of death among gynecological malignancies. In comparison, NPFF and NPFFR2 expression levels were higher in SKOV3 cells than in CaOV3 or OVCAR3 cells Treatment of SKOV3 cells with NPFF did not affect cell viability and proliferation but stimulated cell This study provides evidence that NPFF stimulates EOC cell invasion by upregulating MMP-9 expression

types (neutrophils, T cells, B cells and dendritic cells) and systematically addressed the functional consequences on GPCR-controlled cell migration and tissue localization. Combined depletion of GRK2 and GRK6 in T cells and B cells shows distinct functional outcomes for (a) one GPCR type in different cell types, and (b) different GPCRs in one cell type. ; GRK; T cells; dendritic cells; immune cell trafficking; leukocytes; neutrophils.

combination of brentuximab vedotin and chidamide synergistically suppresses the proliferation of T-cell lymphoma cells through the enhancement of apoptosis Published date November 3, 2023 Abstract " Purpose : Peripheral T-cell lymphoma (PTCL) is an aggressive disease with a poor prognosis. HH cells, originating from an aggressive cutaneous T-cell lymphoma, were used as an experimental model The combined effects of chidamide and BV were demonstrated in a study of HH-cell xenograft mice; mean

Metallo-protease Peptidase M84 from Bacillusaltitudinis induces ROS-dependent apoptosis in ovarian cancer cells This metallo-protease had no discernible impact on normal cell survival, but it specifically induced apoptosis in ovarian cancer cells. PAR-1, a GPCR which is reported to be overexpressed in ovarian cancer cells, was identified as a target This evoked apoptotic death of the ovarian cancer cells through the intrinsic route.

progression in MCF-7 cells Published date March 1, 2024 Abstract "Vasoactive intestinal peptide (VIP In this study, we examined the role of VIPR2 in cell cycle progression. in MCF-7 cells. The percentage of cells in the S-M phase was decreased in MCF-7 cells treated with KS-133. In MCF-7 cells stably-expressing VIPR2, KS-133 decreased PI3K activity and cAMP levels.

pathway by G protein-coupled receptor 37 promotes resistance to cisplatin-induced apoptosis in non-small cell Lung cancer is a major public health concern and represents the most common cause of cancer-related death We detected the expression of GPR37 in NSCLC tissues and cell lines. The study explored the influence of GPR37 on tumor cell proliferation. Furthermore, we examined the effects of GPR37 on tumor cell apoptosis and invasion.

Oncology and Immunology The β2-adrenergic receptor associates with CXCR4 multimers in human cancer cells PIE-FCCS can resolve membrane protein density, diffusion, and multimerization state in live cells at into multimeric complexes larger than dimers in MDA-MB-231 human breast cancer cells and in HCC4006 human lung cancer cells. than in COS-7 and CHO cells and in a ligand-dependent manner.

Immunology Systems Pharmacodynamic Model of Combination Gemcitabine and Trabectedin in Pancreatic Cancer Cells Part II: Cell Cycle, DNA Damage Response, and Apoptosis Pathways Published date October 31, 2023 Abstract , we reported the synergistic cytotoxic effects of gemcitabine and trabectedin on pancreatic cancer cells Here we describe drug effects on pathways associated with cell cycle, DNA damage response (DDR), and The SPD model was subsequently incorporated into our previously-established cell cycle model, forming

< GPCR News < GPCRs in Oncology and Immunology GPR4 in the pH-dependent migration of melanoma cells in pH-gradient) is a well-known driver of tumor progression and metastasis. In this study, we investigated the pH-dependent migration of GPR4 wildtype/overexpressing SK-Mel-28 cells Migration of GPR4 overexpressing SK-Mel-28 cells was enhanced in a range of pH 6.5 - pH 7.5 as compared Results indicate that GPR4 is involved in the migration of melanoma cells, especially in the tumor periphery

in Oncology and Immunology LPA1-mediated inhibition of CXCR4 attenuates CXCL12-induced signaling and cell Results: We observed that CXCR4 forms heteromers with LPA1 in recombinant HEK293A cells and the human breast cancer cell line MDA-MB-231. MDA-MB-231 cells but not in LPA1-deficient cells. have been evidenced across various cell lines.

< GPCR News < GPCRs in Oncology and Immunology Activation of orphan receptor GPR132 induces cell differentiation Here, we showed that genetic activation of the orphan GPCR GPR132 significantly induced cell differentiation Notably, GPR132 activation by 8GL promoted differentiation and reduced colony formation in human AML cell We further showed that the combination of 8GL and an mTOR inhibitor synergistically elicited AML cell impaired tumor growth and extended mouse survival in an AML xenograft model accompanied by enhanced cell

GPCR News < GPCRs in Oncology and Immunology G protein coupled receptor transcripts in human immune cells We have used the method to profile GPCR transcripts in white blood cells (WBCs)-B, CD4, CD8, NK, and dendritic cells; monocytes, and macrophage-like monocytes treated with granulocyte-macrophage colony-stimulating factor-as well as platelets. On average, the white cells studied expressed 160 receptor mRNAs (range, 123-206).

News < GPCRs in Oncology and Immunology The orphan G protein-coupled receptor 141 expressed in myeloid cells High GPR141 messenger RNA levels were expressed in myeloid-lineage cells, including neutrophils (CD11b Gpr141 -/- mice, which we independently generated, displayed almost no abnormalities in myeloid cell myelin oligodendrocyte glycoprotein 35-55-specific T cells. , experimental autoimmune encephalomyelitis , monocytes , myeloid cells .

News < GPCRs in Oncology and Immunology Purinergic GPCR-integrin interactions drive pancreatic cancer cell P2RY2 presents as the purinergic gene with the strongest association with hypoxia, the highest cancer cell-specific receptor-integrin interactions were required for effective downstream signalling, leading to cancer cell Hemant M Kocher , Sabrina Simoncelli , Peter J McCormick , Richard Philip Grose Tags cancer biology , cell Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call for GPCR

< GPCR News < GPCRs in Oncology and Immunology The EBI2 receptor is coexpressed with CCR5 in CD4+ T cells Methods: We identified GPCRs expressed in primary CD4+CCR5+ T cells by multi-RT-qPCR. Cell lines expressing EBI2 were established by transduction with HIV vectors. The amount of HIV reverse transcripts was similar in cells expressing or not EBI2. Conclusions: EBI2 expression in CD4+CCR5+ cells boosts HIV-1 R5 productive infection.

in Oncology and Immunology Vasoactive intestinal peptide receptor 2 signaling promotes breast cancer cell cell lines, promoted cell proliferation. proliferation in VIPR2-overexpressing MCF-7 and MDA-MB-231 cells. was greater than that in control cells, suggesting the increased PKA activity. at the same level as observed in EGFP-expressing cells treated with U0126.

< GPCR News < GPCRs in Oncology and Immunology The GPCR-Gαs-PKA signaling axis promotes T cell dysfunction Here, we cross-integrated large singe-cell RNA-sequencing datasets from CD8+ T cells covering 19 distinct cancer types and identified an enrichment of Gαs-coupled GPCRs on exhausted CD8+ T cells. These include EP2, EP4, A2AR, β1AR and β2AR, all of which promote T cell dysfunction. Gαs-DREADD to activate CD8-restricted Gαs signaling and show that a Gαs-PKA signaling axis promotes CD8+ T cell

and Immunology Dynamic Phosphoproteomics of BRS3 Activation Reveals the Hippo Signaling Pathway for Cell The lung cancer cell line H1299-BRS3 was treated with MK-5046, an agonist of BRS3, for different durations dephosphorylation and nucleus localization of the Yes-associated protein (YAP), and its association with cell Our data collectively demonstrate that BRS3 activation contributes to cell migration through downregulation , Hua Xiao Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

activation of CXC chemokine receptor 4 and histamine receptor H1 enhances calcium signaling and cancer cell Here, we show that HRH1 is widely expressed in various cancer cell lines and cancer tissues and that that endogenously express CXCR4 and HRH1 but not in cells deficient in CXCR4 or HRH1. while histamine alone does not induce cell migration. Enhanced calcium signaling and cell migration are also observed in NCI-H23 and HeLa cells, which coexpress

< GPCR News < GPCRs in Oncology and Immunology Molecular characterization of breast cancer cell pools Methods: We disrupted the PR gene (PGR) in ERα-positive/PR-positive T-47D cells using the CRISPR/Cas9 to control T-47D cells. We analyzed the gene expression profiles of PR-low and control T-47D cells in the absence of hormone More than 6000 genes were DE in control T-47D cells upon stimulation with P4 or P4 plus E2.

and Immunology Opposite Effects of Src Family Kinases on YAP and ERK Activation in Pancreatic Cancer Cells IGF-1 receptors and G protein-coupled (GPCR) signaling systems leading to mitogenic signaling in PDAC cells agonist neurotensin induced rapid activation of Src family of tyrosine kinases (SFK) within PANC-1 cells by FAK phosphorylation at Tyr576/577 and Tyr861, sensitive biomarkers of SFK activity within intact cells and suppressed the growth of Mia PaCa-2 cells xenografted into the flank of nude mice.

expression of GPR50 promotes the proliferation, migration and autophagy of hepatocellular carcinoma cells was analyzed in HCC patients (gene expression omnibus database (GEO) (GSE45436)) and detected in HCC cell 7919, and the results showed that GPR50 was significantly up-regulated in HCC patients and CBRH-7919 cell Gpr50 cDNA was transfected into HCC cell line CBRH-7919, and we found that Gpr50 promoted the proliferation Cuifang Chang Source Contribute to the GPCR News Coming soon Become a Contributor Classified GPCR News Call

line model of LFA-1-stimulated T-cell migration. to assess the contribution of 1109 genes to LFA-1-mediated T-cell polarity and migration. Knockdown of ICAP-1 expression in primary T cells revealed a role in cell polarity, cell velocity and and provides potential novel targets for modulation of the T-cell immune response." , cell migration , cell polarity Source Contribute to the GPCR News Coming soon Become a Contributor