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formation in human intestinal dendritic cells Published date October 29, 2024 Abstract "The intestinal lumen is rich in gut microbial metabolites that serve as signaling molecules for gut immune cells. cells (cDC1s) in the lamina propria of the human intestine. Using human induced pluripotent stem cell-derived cDC1s and a monolayer human gut organoid coculture cell , transepithelial dendrite formation Source < Previous Next > Classified GPCR News GPCR Weekly

+ Gr1+), monocytes (CD11b + Gr1-Ly6C+ and CD11b + Gr1-Ly6C-), macrophages (F4/80+), and dendritic cells Gpr141 -/- mice showed increased CD11b + Gr1+ neutrophils, CD11b + Gr1- monocytes, CD11c+ dendritic cells Moreover, CD11c+ dendritic cells (DCs) purified from Gpr141 -/- mice increased cytokine production of myelin oligodendrocyte glycoprotein 35-55-specific T cells. cells , experimental autoimmune encephalomyelitis , monocytes , myeloid cells .

types (neutrophils, T cells, B cells and dendritic cells) and systematically addressed the functional one GPCR type in different cell types, and (b) different GPCRs in one cell type. Lastly, we provide evidence that complete GRK deficiency impairs dendritic cell homeostasis, which unexpectedly results from defective dendritic cell differentiation and maturation in vitro and in vivo . ; GRK; T cells; dendritic cells; immune cell trafficking; leukocytes; neutrophils.

< GPCR News < GPCRs in Oncology and Immunology CCL5-producing migratory dendritic cells guide CCR5+ monocytes into the draining lymph nodes Published date March 21, 2023 Abstract "Dendritic cells (DCs) and monocytes capture, transport, and present antigen to cognate T cells in the draining lymph nodes (LNs) in a CCR7 and molecular insights into GPCR function Contributors Articles GPCR Events, Meetings, and Webinars Call

September 10, 2024 Abstract "Skin psoriasis is defined as receiving external stimulation to activate skin dendritic well as induce T helper 17 (Th17) cell differentiation leading to elevated production of interleukin In this research, our team allocated GPR97 depletion (GPR97-/-), GPR97 conditional depletion on dendritic In addition, hyperproliferative keratinocytes as well as accumulation of DCs and Th17 cells were detected abnormal proliferation as well as Th17 cells differentiation.

GPCR News < GPCRs in Oncology and Immunology G protein coupled receptor transcripts in human immune cells We have used the method to profile GPCR transcripts in white blood cells (WBCs)-B, CD4, CD8, NK, and dendritic cells; monocytes, and macrophage-like monocytes treated with granulocyte-macrophage colony-stimulating factor-as well as platelets. On average, the white cells studied expressed 160 receptor mRNAs (range, 123-206).

These receptors are expressed in almost all cells. P2Y receptors are found in immune cells, such as macrophages, neutrophils, mast cells, dendritic cells P2Y receptors play essential roles in inflammation and are involved in several cell processes, including and molecular insights into GPCR function Contributors Articles GPCR Events, Meetings, and Webinars Call

Whereas it is well established that single-cell migration is often guided by gradients of chemokines Upon exposure to the CCR7 ligand CCL19, dendritic cells (DCs) effectively internalize the receptor and patterns to the size and geometry of the environment, and provide a guidance cue for other comigrating cells dual role of CCR7 as a GPCR that both senses and consumes its ligand can thus provide a novel mode of cellular and molecular insights into GPCR function Contributors Articles GPCR Events, Meetings, and Webinars Call

< GPCR News < GPCRs in Oncology and Immunology PAR2 on oral cancer cells and nociceptors contributes PAR2 is a G protein-coupled receptor (GPCR) expressed on neurons and cells in the cancer microenvironment We focused on the differential contribution of PAR2 on oral cancer cells and neurons to oral cancer pain We report that F2RL1 was overexpressed in human oral cancers and cancer cell lines. Deletion of F2RL1 on cancer cells reduced cancer-associated mechanical allodynia.

Here, we showed that US28 increased the malignancy of U251 glioblastoma cells by enhancing signaling Enhanced S1P signaling promoted glioblastoma cell proliferation and survival by activating the kinases US28 also activated the S1P signaling axis in HCMV-infected cells. Authors Nick D Bergkamp , Jeffrey R van Senten , Hendrik J Brink , Maarten P Bebelman , Jelle van den and molecular insights into GPCR function Contributors Articles GPCR Events, Meetings, and Webinars Call

Registration Logo Contest Committee Sponsors GPCR Retreat Program < Back to schedule Interaction with the cell adhesion molecule NEGR1 affects mGluR5 cell signalling Date & Time Friday, November 3rd / 1:30 PM Abstract Ribeiro has supervised eleven M.Sc. and six Ph.D. students, as well as five post-doctorate fellows. Nowadays, her research group comprises four undergraduates, two M.Sc., and six Ph.D. students, as well These drugs were shown to be very effective to rescue the cell death observed in a mouse model of Huntington

Oncology and Immunology The β2-adrenergic receptor associates with CXCR4 multimers in human cancer cells PIE-FCCS can resolve membrane protein density, diffusion, and multimerization state in live cells at into multimeric complexes larger than dimers in MDA-MB-231 human breast cancer cells and in HCC4006 human lung cancer cells. than in COS-7 and CHO cells and in a ligand-dependent manner.

roles of AGPCRs in the periphery ADGRG1/GPR56 regulates survival of terminally differentiated CD8+ T cells health and disease Douglas Tilley ADGRG1/GPR56 regulates survival of terminally differentiated CD8+ T cells Centre for Experimental Medicine, Institute for Biocehmistry and Molecular Cell Biology, University Medical Diabetes and Cancer, Helmholtz Diabetes Centre, Munich 2012 - 2015 Postdoctoral fellow, Department of Cell impact cardiac structure and function, and has evolved to encompass their roles in regulating immune cell

< GPCR News < GPCRs in Oncology and Immunology GPR132 regulates the function of NK cells through the "As a member of the proton-sensing GPCR receptors, GPR132 plays a crucial role in regulating immune cell Mechanically, GPR132 regulates NK cell function through the CSK/ZAP70/NF-κB signaling axis. functional execution of CAR-NK cells against colorectal cancer. and that inhibition of GPR132 provided an updated insight for NK cell therapy."

in Oncology and Immunology Autocrine proteinase-activated receptor signaling in PC3 prostate cancer cells PARs are highly expressed in many cancer cells, including prostate cancer (PCa), and regulate various In this study, we examined the androgen-independent human prostatic cancer cell line PC3 and find the Using genetically encoded PAR cleavage biosensors, we showed that PC3 cells secrete proteolytic enzymes of PAR1 promotes PC3 cell migration and suppresses cell proliferation, whereas PAR2 deficiency showed

progression in MCF-7 cells Published date March 1, 2024 Abstract "Vasoactive intestinal peptide (VIP In this study, we examined the role of VIPR2 in cell cycle progression. in MCF-7 cells. The percentage of cells in the S-M phase was decreased in MCF-7 cells treated with KS-133. In MCF-7 cells stably-expressing VIPR2, KS-133 decreased PI3K activity and cAMP levels.

line model of LFA-1-stimulated T-cell migration. to assess the contribution of 1109 genes to LFA-1-mediated T-cell polarity and migration. Knockdown of ICAP-1 expression in primary T cells revealed a role in cell polarity, cell velocity and and provides potential novel targets for modulation of the T-cell immune response." , cell migration , cell polarity Source < Previous Next > Classified GPCR News GPCR Weekly News GPCR

in Oncology and Immunology Vasoactive intestinal peptide receptor 2 signaling promotes breast cancer cell cell lines, promoted cell proliferation. proliferation in VIPR2-overexpressing MCF-7 and MDA-MB-231 cells. was greater than that in control cells, suggesting the increased PKA activity. at the same level as observed in EGFP-expressing cells treated with U0126.

activation of CXC chemokine receptor 4 and histamine receptor H1 enhances calcium signaling and cancer cell Here, we show that HRH1 is widely expressed in various cancer cell lines and cancer tissues and that that endogenously express CXCR4 and HRH1 but not in cells deficient in CXCR4 or HRH1. while histamine alone does not induce cell migration. Enhanced calcium signaling and cell migration are also observed in NCI-H23 and HeLa cells, which coexpress

< GPCR News < GPCRs in Oncology and Immunology NPFF stimulates human ovarian cancer cell invasion by The TaqMan probe-based RT-qPCR showed that NPFF and NPFFR2 were expressed in three human EOC cells, CaOV3 In comparison, NPFF and NPFFR2 expression levels were higher in SKOV3 cells than in CaOV3 or OVCAR3 cells Treatment of SKOV3 cells with NPFF did not affect cell viability and proliferation but stimulated cell This study provides evidence that NPFF stimulates EOC cell invasion by upregulating MMP-9 expression

GPCRs in Oncology and Immunology Toxin protein LukS-PV targeting complement receptor C5aR1 inhibits cell This project aims to investigate the role of LukS-PV on HCC cell proliferation and explore underlying Our findings revealed that LukS-PV targeting C5aR1 inhibited HCC cell proliferation in vitro and in vivo Interestingly, we discovered that LukS-PV inhibited the proliferation of HCC cells by upregulating the Collectively, Our findings revealed that LukS-PV targeting C5aR1 inhibits HCC cell proliferation through

G protein βγ subunits inhibitor, suppresses the TGF-α-induced migration of hepatocellular carcinoma cells Abstract "G protein-coupled receptor (GPCR) signaling regulates a wide range of pathophysiological cell transforming growth factor-α (TGF-α) induces the migration of human hepatocellular carcinoma (HCC) HuH7 cells This study aims to determine whether Gβγ subunits regulate the TGF-α-induced migration of HCC HuH7 cells Gallein significantly reduced the TGF-α-induced cell migration.

< GPCR News < GPCRs in Oncology and Immunology miR-19a may function as a biomarker of oral squamous cell Using a transcriptomic analysis of over 37,000 nuclei, we identified twelve distinct clusters of cells corresponding to temperature-sensing arista neurons, humidity-sensing sacculus neurons, and support cells We found that each cell type could be characterized by a unique expression profile of ion channels, GPCR signaling molecules, synaptic vesicle cycle proteins, and cell adhesion molecules.

< GPCR News < GPCRs in Oncology and Immunology A2aR on lung adenocarcinoma cells: A novel target for 2a receptor (A2aR), a typical GPCR with a high affinity for adenosine, is widely expressed on immune cells Here, we identify that A2aR is specifically expressed on tumor cells from lung adenocarcinoma (LUAD) We hypothesize that blocking A2aR on LUAD cells will inhibit the role of TAMs and control tumor growth Constructing models of TAMs and LUAD mice, we find that A2aR highly expressed on LUAD cells promotes

Immunology Systems Pharmacodynamic Model of Combination Gemcitabine and Trabectedin in Pancreatic Cancer Cells Part II: Cell Cycle, DNA Damage Response, and Apoptosis Pathways Published date October 31, 2023 Abstract , we reported the synergistic cytotoxic effects of gemcitabine and trabectedin on pancreatic cancer cells Here we describe drug effects on pathways associated with cell cycle, DNA damage response (DDR), and The SPD model was subsequently incorporated into our previously-established cell cycle model, forming

and Immunology Opposite Effects of Src Family Kinases on YAP and ERK Activation in Pancreatic Cancer Cells IGF-1 receptors and G protein-coupled (GPCR) signaling systems leading to mitogenic signaling in PDAC cells agonist neurotensin induced rapid activation of Src family of tyrosine kinases (SFK) within PANC-1 cells by FAK phosphorylation at Tyr576/577 and Tyr861, sensitive biomarkers of SFK activity within intact cells and suppressed the growth of Mia PaCa-2 cells xenografted into the flank of nude mice.

< GPCR News < GPCRs in Oncology and Immunology The EBI2 receptor is coexpressed with CCR5 in CD4+ T cells Methods: We identified GPCRs expressed in primary CD4+CCR5+ T cells by multi-RT-qPCR. Cell lines expressing EBI2 were established by transduction with HIV vectors. The amount of HIV reverse transcripts was similar in cells expressing or not EBI2. Conclusions: EBI2 expression in CD4+CCR5+ cells boosts HIV-1 R5 productive infection.

Metallo-protease Peptidase M84 from Bacillusaltitudinis induces ROS-dependent apoptosis in ovarian cancer cells This metallo-protease had no discernible impact on normal cell survival, but it specifically induced apoptosis in ovarian cancer cells. PAR-1, a GPCR which is reported to be overexpressed in ovarian cancer cells, was identified as a target This evoked apoptotic death of the ovarian cancer cells through the intrinsic route.