Immunology S1P Signaling Genes as Prominent Drivers of BCR-ABL1-Independent Imatinib Resistance and Six Herbal Compounds as Potential Drugs for Chronic Myeloid Leukemia Methods & Updates in GPCR Research High-throughput

November 2021 " Chronic use of most opioids causes tolerance; the new compounds avoid this and other JUPITER, FL— Scientists at Scripps Research in Florida have created a collection of new pain-relieving compounds

October 2022 "Study of small molecule binding to live cells provides important information on the characterization of ligands pharmacologically. Here we developed and validated a label-free, liquid chromatography-mass spectrometry (LC-MS) based cell binding assay, using centrifugation to separate binders from non-binders. This assay was applied to various target classes, with particular emphasis on those for which protein-based binding assay can be difficult to achieve. In one example, to study a G protein coupled receptor (GPCR), we used one antagonist as probe and multiple other antagonists as competitor ligands. Binding of the probe was confirmed to be specific and saturable, reaching a fast equilibrium. Competition binding analysis by titration of five known ligands suggested a good correlation with their inhibition potency. In another example, this assay was applied to an ion channel target with its agonists, of which the determined binding affinity was consistent with functional assays. This versatile method allows quantitative characterization of ligand binding to cell surface expressed targets in a physiologically relevant environment." Read more at the source #DrGPCR #GPCR #IndustryNews

GPCR Retreat Program < Back to schedule Cannabinoid compounds to augment L-DOPA treatment in Parkinson's

Committee Sponsors GPCR Retreat Program < Back to schedule Distinct sub-cellular signal propagation as a component

fluorescence-based kinetic binding assays specifically designed to assess the kinetics of unlabelled compounds