Immunology Identification of a G-protein coupled receptor-related gene signature through bioinformatics analysis GPCRRGs were screened using least absolute shrinkage and selection operator (LASSO) Cox regression analysis The predictive ability of the model was evaluated by Kaplan-Meier (K-M) survival analysis. gene set enrichment analysis (ssGSEA) and CIBERSORT. K-M analysis of the prognostic model showed that it could differentiate high- and low-risk patients,

partners across cancer subtypes and are associated to specific transcriptional programs and to patient survival cell lines , cell-cell communication , drug repurposing , personalized medicine , signaling network , survival analysis , transcriptomics .

< GPCR News < GPCRs in Oncology and Immunology Prediction of survival and immunotherapy response by the Real-time PCR analysis, bioinformatic analysis and luciferase assays were performed to establish a potential Moreover, in silico analysis confirmed a potential binding site of miR-19a on the 3'UTR of GRK6 mRNA,

Kruskal-Wallis test, Wilcoxon sign-rank test, logistic regression, Kaplan-Meier method, Cox regression analysis , Gene set enrichment analysis (GSEA), and immuno-infiltration analysis were used to evaluate the relationship Low PAXIP1-AS1 expression predicted a poorer overall survival (OS) (HR: 0.71; 95% CI: 0.55-0.92; P = ), progression free interval (PFS) (HR: 1.776; 95% CI: 1.067-2.955; P = 0.001) and disease specific survival Low expression of PAXIP1-AS1 was significantly associated with poor survival and immune infiltration

Published date September 15, 2023 Abstract "The multikinase inhibitor sorafenib improves event-free survival One-year overall survival (OS) and EFS were 80% and 76%, without differences in MRD- CR rates, OS, or sorafenib with those of a matched cohort of 76 patients treated with CLAG-M alone, multivariable-adjusted survival Benefit was limited to patients with intermediate-risk disease (univariate analysis: P = .01 for OS;

< GPCR News < GPCRs in Oncology and Immunology Comparative Analysis of the GNAI Family Genes in Glioblastoma 2023 Abstract Glioblastoma multiforme (GBM) is one of the most aggressive cancers with a low overall survival MetaCore and gene ontology (GO) analyses were conducted to explore the role of GNAI3 in co-expressed genes and associated signaling pathways using a transcript analysis. A single-cell analysis was used to assess GNAI3 expression in GBM.

Methods: Bioinformatics analysis was performed to assess the correlation between EGFR and SSTR2 based Results: The bioinformatics analysis and IHC showed a positive correlation between SSTR2 and EGFR in expression of EGFR, and high coexpression of SSTR2 and EGFR had a poorer prognosis in both progression-free survival (PFS) and overall survival (OS). Cox multivariate analysis identified SSTR2 and EGFR as independent poor predictors of PFS.

March 21, 2023 Abstract " Pancreatic ductal adenocarcinoma (PDAC) continues to show no improvement in survival Analysing the main members of the PDAC extracellular purinome using publicly available databases discerned which members may impact patient survival. association with hypoxia, the highest cancer cell-specific expression and the strongest impact on overall survival

Despite decades of research, the survival rates for GBM remain effectively stagnant. Cell survival assays, via nuclei counting and cell titer glo, were used to demonstrate sensitivity to To determine GPR68 inhibition's mechanism of cell death we use DAVID pathway analysis of RNAseq. Lastly, we use siRNA and CRISPRi to demonstrate the critical role of ATF4 suppression via GPR68 for GBM survival

Exploration of prognostic and treatment markers in hepatocellular carcinoma via GPCR-related genes analysis Univariant and multivariant Cox regression analyses, as well as least absolute shrinkage and selection Differential expression and functional enrichment analyses were performed; protein-protein interaction Areas under the curves (AUC) corresponding to one-, three- and five-year survival were 0.731, 0.765 and

Patients with ERα-positive/PR-negative tumors have shorter disease-free and overall survival than patients We analyzed the gene expression profiles of PR-low and control T-47D cells in the absence of hormone genes between experimental groups were characterized based on RNA-seq and Gene Ontology (GO) enrichment analyses GO analysis revealed seven significantly enriched biological processes affected by PR and associated

Abstract "The ability of animals to perceive and respond to sensory information is essential for their survival Using a transcriptomic analysis of over 37,000 nuclei, we identified twelve distinct clusters of cells

This metallo-protease had no discernible impact on normal cell survival, but it specifically induced of Peptidase M84 in syngeneic mice significantly diminished ascites accumulation, increasing murine survival

Analyzing 899 CRC tissues revealed elevated RGS16 levels, correlating with clinicopathological features protein level in CRC, and found that patients with RGS16-high tumors exhibited decreased disease-specific survival (DSS) and disease-free survival (DFS) compared to those with low RGS16 expression.

Advanced data analysis for GPCR pharmacology Dr. Understanding the pharmacology data analysis framework we use for GPCRs. Appreciating the practical realities, limitations, and tradeoffs of GPCR data analysis. Modules: Module 1 - Concentration-response analysis: Become a CRC super-user. Module 3 - Antagonist pharmacology and binding assay analysis.

In the present study, expression analyses of GPR176 are performed in patients with colorectal cancer. A positive correlation between GPR176 upregulation and the proliferation and poor overall survival of

Our earlier transcriptomic analysis identified Urothelial Carcinoma Associated-1 (UCA1) as an LPA-stimulated UCA1 expression inversely correlates with survival outcomes and therapy response in ovarian cancer clinical

We sought to profile the expression of PRL3 in pediatric tumors in relation to survival outcomes, expression PRL3 was expressed in 75% of relapsed tumors and associated with shorter median event-free survival.

gene expression and its significance in relation with the clinicopathological features and overall survival Methods: Immunohistochemistry, western blot and quantitative real-time PCR techniques were used to analyze habits (p = 0.045), histological differentiation (p = 0.013), clinical TNM stages (p = 0.014), and poor survival

Session VIII Physiological and pathological roles of AGPCRs in the periphery ADGRG1/GPR56 regulates survival Georgiadi ADGRF5-mediated regulation of cardiac health and disease Douglas Tilley ADGRG1/GPR56 regulates survival

reveal the spatial and temporal requirements for GPCR activation of signaling pathways related to cell survival

Oncology and Immunology GPR37 expression as a prognostic marker in gliomas: a bioinformatics-based analysis Immunohistochemical staining of clinical samples were used to verify the speculations of bioinformatics analysis

GPCR BAI1/ADGRB1 can block IGF1R-mediated growth signalling, increase radiosensitivity and augment survival GPCR BAI1/ADGRB1 can block IGF1R-mediated growth signalling, increase radiosensitivity and augment survival tumor development and how to use this knowledge to devise new therapeutics that will improve patient survival

UNC-CH, where she was the first to discover the essential role of adrenomedullin peptide for embryonic survival

alone or in combination with an mTOR inhibitor remarkably impaired tumor growth and extended mouse survival

Enhanced S1P signaling promoted glioblastoma cell proliferation and survival by activating the kinases

< GPCR News < GPCRs in Oncology and Immunology Transcriptomic analysis of human cytomegalovirus to survey The resulted raw data were analyzed by conventional RNA-Seq software to determine the Differentially Afterward, Gene Ontology (GO) and pathway enrichment analyses were conducted to determine the enriched Result: The analysis of RNA-Seq data related to RT patients with HCMV active viremia led to the identification KEGG pathway analysis revealed the enrichment of DEGs in IL18 signaling, AGE-RAGE signaling pathway in

< GPCR News < GPCRs in Oncology and Immunology Pan-cancer functional analysis of somatic mutations in A Two-Entropy Analysis confirmed the correlation between residue conservation and cancer-related mutation